DECHLORINATION OF 2,4,6-TRICHLOROPHENOL BY FREE AND IMMOBILIZED LACCASE FROM TRAMETES VERSICOLOR IN A LAB SCALE BIOREACTOR

Year 2011, Volume: 1 Issue: 2, 113 - 24, 06.07.2011

Arzu Ünal Ahmet Çabuk , Nazif Kolonkaya

Abstract

Detoxification of a chlorinated phenolic compound, 2,4,6-trichlorophenol through treatment with laccase enzyme produced by a white rot fungus, Trametes versicolor was investigated. Enzymatic dechlorination experiments by using free and immobilized laccase have been performed in a lab scale bioreactor. Chlorine ion and dissolved oxygen electrodes mounted to the bioreactor were used continuously to detect the profiles of chlorine ions and oxygen consumption, respectively, in reaction medium.
The maximum dechlorination activity of laccase for free and immobilized form was determined as 160 μM of substrate concentration at pH 5.0, 25 °C, and 30 min of incubation time. Also, GC/MS analyses of enzymatic degradation products indicated that chlorine removal was a result of degradation of 2,4,6-trichlorophenol by the laccase under the determined optimum conditions.

Keywords

Laccase, Trametes versicolor, 2,4,6-tricholorophenol, Dechlorination

LABORATUAR ÖLÇEKLI BIYOREAKTÖRDE TRAMETES VERSICOLOR’DAN ELDE EDILEN SERBEST VE IMMOBILIZE LAKKAZ ILE 2,4,6- TRICHLOROPHENOL’DEN KLOR UZAKLAŞTIRILMASI

Abstract

Bu çalışmada, klorlu fenolik bileşiklerden 2,4,6-triklorofenolün Trametes versicolor olarak bilinen beyaz çürükçül fungusun ürettiği lakkaz enzimi ile muamele edilerek detoksifikasyonu araştırılmıştır. Serbest ve immobilize enzim kullanılarak yapılan enzimatik deklorinasyon deneyleri laboratuar ölçeğinde bir reaktörde gerçekleştirilmiştir. Biyoreaktöre monte edilmiş klor iyon ve çözünmüş oksijen elektrodları, sürekli olarak reaksiyon ortamında klor ve çözünmüş oksijen değişimlerini belirlemek amacıyla kullanılmıştır. Serbest ve immobilize formda lakkazın en yüksek deklorinasyon aktivitesi; substrat konsantrasyonu 160 mM, pH 5.0, reaksiyon süresi 30 dakika ve sıcaklık olarak da 25 °C’de bulunmuştur. Ayrıca klor uzaklaştırılmasının gösterildiği enzimatik yıkım ürünlerinin GC/MS analizi, belirlenen optimum şartlarda lakkazla 2,4,6-triklorofenolün parçalandığı sonucunu vermiştir

Keywords

Lakkaz, Trametes versicolor, 2, 4, 6-tricholorophenol, Klor uzaklaştırılması

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