BibTex RIS Kaynak Göster

DECHLORINATION OF 2,4,6-TRICHLOROPHENOL BY FREE AND IMMOBILIZED LACCASE FROM TRAMETES VERSICOLOR IN A LAB SCALE BIOREACTOR

Yıl 2011, Cilt: 1 Sayı: 2, 113 - 24, 06.07.2011

Öz

Detoxification of a chlorinated phenolic compound, 2,4,6-trichlorophenol through treatment with laccase enzyme produced by a white rot fungus, Trametes versicolor was investigated. Enzymatic dechlorination experiments by using free and immobilized laccase have been performed in a lab scale bioreactor. Chlorine ion and dissolved oxygen electrodes mounted to the bioreactor were used continuously to detect the profiles of chlorine ions and oxygen consumption, respectively, in reaction medium.
The maximum dechlorination activity of laccase for free and immobilized form was determined as 160 μM of substrate concentration at pH 5.0, 25 °C, and 30 min of incubation time. Also, GC/MS analyses of enzymatic degradation products indicated that chlorine removal was a result of degradation of 2,4,6-trichlorophenol by the laccase under the determined optimum conditions.

Kaynakça

  • Aktaş, N., Çiçek, H., Ünal Taşpınar, A., Kibarer, G., Kolankaya, N. and Tanyolaç, A. (2001). Reaction kinetics for laccase- catalyzed polymerization of 1-naphthol. Bioresource Technol 80, 29–36.
  • Arcand, R.L. and Archibald, F.S. (1991). Direct dechlorination of chlorophenolic com- pounds by laccases from Trametes (Coriolus) versicolor. Enzyme Microb. Technol 13, 194–203.
  • Arora, D.S., Chander, M. and Gill, P.K. (2002). of Involvement dase,manganese peroxidase and laccase in degradation and selective ligninolysis of wheat straw. Int. Biodeter. Biodegr 50, 115–120. lignin peroxi
  • Barr, D.P. and. Aust, S.D. (1994). Enzyme deg- radation of lignin. Rev. Environ. Contam. Toxicol 138, 49–72.
  • Cabuk, A., Unal, A.T. and Kolankaya, N. (2006). Biodegradation of cyanide by a white rot fungus, Trametes versicolor. Biotechnol Lett 28, 1313–1317.
  • Choi, J., Kim, Y. and Choi, S.J. (2007). Reduc- tive dechlorination and biodegradation of 2,4,6-trichlorophenol using sequential permeable reactive barriers: Laboratory studies, Chemosphere 67, 1551–1557.
  • Duran, N. and Esposito, E. (2000). Potantial ap- plications of oxidative enzymes and phenoloxidase-like compounds in waste- water and soil treatment, Review. Appl. Catal. B- Environ 28, 83–99.
  • Ehlers, G.A. and Rose, P.D. (2005). Immobi- lized white-rot fungal biodegradation of phenol and chlorinated phenol in trickling packed-bed reactors by employing se- quencing batch operation. Bioresource Technol 96, 1264–1275.
  • Hofrichter, M. (2002). Review: lignin conver- sion by manganese peroxidase (MnP). Enzyme Microb. Tech 30, 454–466.
  • Kirk, T.K. and Farrel, R.L. (1987). Enzymatic "Combustion": The microbial degradation of lignin. Annu. Rev. Microbiol 41, 465– 505.
  • Krcmar, P., Kubatova, A., Votruba, J., Er- banova, P. and Novotny, C. (1999). Deg- radation of polychlorinated biphenyls by extracellular enzymes of Phanerochaete chrysosporium produced in a perforated plate bioreactor. World J. Microb. Biot 15, 249–276.
  • Kumaran, P. and Paruchuri, Y.L. (1997). Kinet- ics of phenol biotransformation. Water Res 31,11–22.
  • Leontievsky, AA., Myasoedova, NM., Bas- kunov, BP., Golovleva, LA., Bucke, C. and Evans, C.S. (2001). Transformation of 2,4,6-trichlorophenol by free and im- mobilized fungal laccase, Appl. Micro- biol. Biotechnol 57, 85–91.
  • Limura, Y., Hartikainen, P. and Tatsumi, K. (1996). chloroguaiacol by laccase of white rot basidomycete Coriolus versicolor. Appl. Microbiol. Biot 45, 434–439. of tetra
  • Lisov, A.V., Pozhidaeva, Z.A., Stepanova, E.V., Koroleva, O.V. and Leontievsky, AA. (2007), Conversion of Polychlorophenols by Laccases with 1-Hydroxybenzotriazole as a Mediator, Appl. Biochem. Microbiol 43, 616–619.
  • Majumder, P.S. and Gupta, S.K. (2007). Re- moval of chlorophenols in sequential an- aerobic–aerobic reactors. Bioresource Technol 98,118–129.
  • Martinez-Ruiz, J., Parra, M., Tomás, V., Marti- nez-Gutiérrez, R., García-Canovas, F. and Tudela, J. (2009). Biodegradation of 2,4,6-trichlorophenol catalysed by the me- thylsyringate–laccase mediator system, New Biotechnol. 25, 160.
  • Michizoe, J., Goto, M. and Furusaki, S. (2001). Catalytic activity of laccase hosted in re- versed micelles, J. Biosci. Bioeng 92, 67– 71.
  • Novotny, C., Svobodova K., Erbanova, P., Ca- jthamla, T., Kasinatha, A., Lang, E. and Sasek, V. (2004). Ligninolytic fungi in bioremediation: extracellular enzyme pro- duction and degradation rate. Soil Biol. Biochem 36, 1545–1551.
  • Pallerla, S. and Chambers, R.P. (1998). Reactor development for biodegradation of penta- chlorophenol. Catal. Today 40, 103–111.
  • Reddy, G.V.B., Sollewijn Gelpke, M.D. and Gold, M.H. (1998). Degradation of 2,4,6- Trichlorophenol by Phanerochaete chry- sosporium: Involvement of Reductive Dechlorination. J. Bacteriol. 180, 5159– 5164.
  • Rozie, H., Somers, W., Bonte, A., Visser, J., Van’t Riet, K. and Rombouts, F.M. (1988). Adsorption Characteristics of Endo-polygalacturonase Beads. Biotechnol. Appl. Bioc. 10, 346– 358. on Aljinate
  • Taspinar, A. and Kolankaya, N. (1998). Optimi- zation of enzymatic chlorine removal from Kraft pulp. B. Environ. Contam. Tox. 61, 15–21.
  • Thomas, D.R., Carswell, K.S. and Georgiou, G. (1992). Mineralization of biphenyl and PCBs by white rot fungus Phanerochaete chrysosporium. Biotechnol. Bioeng 40, 1395–1402.
  • Tuomela, M., Lyytikainen, M., Oivanena, P. and Hatakka, A., (1999). Mineralization and conversion of pentachlorophenol (PCP) in soil inoculated with the white-rot fungus Trametes versicolor . Soil Biol. Biochem 31, 65–74.
  • Ünal, A. and Kolankaya, N. (2004). Chlorin re- movel from pp’ DDT by laccase enzyme produced from Trametes versicolor. Turk- ish Electronic J. Biotechnol 2, 17–21.
  • Valentin, L., Feijooa, G., Moreirab, M.T. and Lema, J.M., (2006). Biodegradation of polycyclic aromatic hydrocarbons in for- est and salt marsh soils by white-rot fungi. Int. Biodeter. Biodegr 58, 15–21.
  • Walter, M., Boyd-Wilsona, K.S.H., McNaugh- tonb, D. and Northcott, G. (2005). Labo- ratory trials on the bioremediation of aged pentachlorophenol residues. Int. Biodeter. Biodegr 55, 121–130.
  • Yadav, S. and. Reddy, C.A. (1992). Non- involvement of lignin peroxidases and manganese trichlorophenoxyacetic acid degradation by Phanerochaete chrysosporium. Bio- technol. Lett 14, 1089–1092. in 2,4,5
  • Yadav, J.S., Ouensen, J.F., Tiedje, J.F.Q.J.M. and Reddy, C.A. (1995). Degradation of Polychlorinated Biphenyl Mixtures (Aro- clors 1242,1254, and 1260) by the White Rot Fungus Phanerochaete chrysospo- rium as Evidenced by Congener-Specific Analysis. Appl. Environ. Microbiol 2560– 2565.
  • Yarapolov, A.I., Skorobogatko, O.V., Vartanov, S.S. and Varfolomeyev, S.D. (1994). Lac- case properties, catalytic mechanism and applicability. Appl. Biochem. Biotech 49, 257–279.
  • Zouari, H., Labat, M. and Sayadi, S. (2002). Degradation of 4-chlorophenol by the white rot fungus Phanerochaete chryso- sporium in free and immobilized cultures. Bioresource Technol 84,145–150.

LABORATUAR ÖLÇEKLI BIYOREAKTÖRDE TRAMETES VERSICOLOR’DAN ELDE EDILEN SERBEST VE IMMOBILIZE LAKKAZ ILE 2,4,6- TRICHLOROPHENOL’DEN KLOR UZAKLAŞTIRILMASI

Yıl 2011, Cilt: 1 Sayı: 2, 113 - 24, 06.07.2011

Öz

Bu çalışmada, klorlu fenolik bileşiklerden 2,4,6-triklorofenolün Trametes versicolor olarak bilinen beyaz çürükçül fungusun ürettiği lakkaz enzimi ile muamele edilerek detoksifikasyonu araştırılmıştır. Serbest ve immobilize enzim kullanılarak yapılan enzimatik deklorinasyon deneyleri laboratuar ölçeğinde bir reaktörde gerçekleştirilmiştir. Biyoreaktöre monte edilmiş klor iyon ve çözünmüş oksijen elektrodları, sürekli olarak reaksiyon ortamında klor ve çözünmüş oksijen değişimlerini belirlemek amacıyla kullanılmıştır. Serbest ve immobilize formda lakkazın en yüksek deklorinasyon aktivitesi; substrat konsantrasyonu 160 mM, pH 5.0, reaksiyon süresi 30 dakika ve sıcaklık olarak da 25 °C’de bulunmuştur. Ayrıca klor uzaklaştırılmasının gösterildiği enzimatik yıkım ürünlerinin GC/MS analizi, belirlenen optimum şartlarda lakkazla 2,4,6-triklorofenolün parçalandığı sonucunu vermiştir

Kaynakça

  • Aktaş, N., Çiçek, H., Ünal Taşpınar, A., Kibarer, G., Kolankaya, N. and Tanyolaç, A. (2001). Reaction kinetics for laccase- catalyzed polymerization of 1-naphthol. Bioresource Technol 80, 29–36.
  • Arcand, R.L. and Archibald, F.S. (1991). Direct dechlorination of chlorophenolic com- pounds by laccases from Trametes (Coriolus) versicolor. Enzyme Microb. Technol 13, 194–203.
  • Arora, D.S., Chander, M. and Gill, P.K. (2002). of Involvement dase,manganese peroxidase and laccase in degradation and selective ligninolysis of wheat straw. Int. Biodeter. Biodegr 50, 115–120. lignin peroxi
  • Barr, D.P. and. Aust, S.D. (1994). Enzyme deg- radation of lignin. Rev. Environ. Contam. Toxicol 138, 49–72.
  • Cabuk, A., Unal, A.T. and Kolankaya, N. (2006). Biodegradation of cyanide by a white rot fungus, Trametes versicolor. Biotechnol Lett 28, 1313–1317.
  • Choi, J., Kim, Y. and Choi, S.J. (2007). Reduc- tive dechlorination and biodegradation of 2,4,6-trichlorophenol using sequential permeable reactive barriers: Laboratory studies, Chemosphere 67, 1551–1557.
  • Duran, N. and Esposito, E. (2000). Potantial ap- plications of oxidative enzymes and phenoloxidase-like compounds in waste- water and soil treatment, Review. Appl. Catal. B- Environ 28, 83–99.
  • Ehlers, G.A. and Rose, P.D. (2005). Immobi- lized white-rot fungal biodegradation of phenol and chlorinated phenol in trickling packed-bed reactors by employing se- quencing batch operation. Bioresource Technol 96, 1264–1275.
  • Hofrichter, M. (2002). Review: lignin conver- sion by manganese peroxidase (MnP). Enzyme Microb. Tech 30, 454–466.
  • Kirk, T.K. and Farrel, R.L. (1987). Enzymatic "Combustion": The microbial degradation of lignin. Annu. Rev. Microbiol 41, 465– 505.
  • Krcmar, P., Kubatova, A., Votruba, J., Er- banova, P. and Novotny, C. (1999). Deg- radation of polychlorinated biphenyls by extracellular enzymes of Phanerochaete chrysosporium produced in a perforated plate bioreactor. World J. Microb. Biot 15, 249–276.
  • Kumaran, P. and Paruchuri, Y.L. (1997). Kinet- ics of phenol biotransformation. Water Res 31,11–22.
  • Leontievsky, AA., Myasoedova, NM., Bas- kunov, BP., Golovleva, LA., Bucke, C. and Evans, C.S. (2001). Transformation of 2,4,6-trichlorophenol by free and im- mobilized fungal laccase, Appl. Micro- biol. Biotechnol 57, 85–91.
  • Limura, Y., Hartikainen, P. and Tatsumi, K. (1996). chloroguaiacol by laccase of white rot basidomycete Coriolus versicolor. Appl. Microbiol. Biot 45, 434–439. of tetra
  • Lisov, A.V., Pozhidaeva, Z.A., Stepanova, E.V., Koroleva, O.V. and Leontievsky, AA. (2007), Conversion of Polychlorophenols by Laccases with 1-Hydroxybenzotriazole as a Mediator, Appl. Biochem. Microbiol 43, 616–619.
  • Majumder, P.S. and Gupta, S.K. (2007). Re- moval of chlorophenols in sequential an- aerobic–aerobic reactors. Bioresource Technol 98,118–129.
  • Martinez-Ruiz, J., Parra, M., Tomás, V., Marti- nez-Gutiérrez, R., García-Canovas, F. and Tudela, J. (2009). Biodegradation of 2,4,6-trichlorophenol catalysed by the me- thylsyringate–laccase mediator system, New Biotechnol. 25, 160.
  • Michizoe, J., Goto, M. and Furusaki, S. (2001). Catalytic activity of laccase hosted in re- versed micelles, J. Biosci. Bioeng 92, 67– 71.
  • Novotny, C., Svobodova K., Erbanova, P., Ca- jthamla, T., Kasinatha, A., Lang, E. and Sasek, V. (2004). Ligninolytic fungi in bioremediation: extracellular enzyme pro- duction and degradation rate. Soil Biol. Biochem 36, 1545–1551.
  • Pallerla, S. and Chambers, R.P. (1998). Reactor development for biodegradation of penta- chlorophenol. Catal. Today 40, 103–111.
  • Reddy, G.V.B., Sollewijn Gelpke, M.D. and Gold, M.H. (1998). Degradation of 2,4,6- Trichlorophenol by Phanerochaete chry- sosporium: Involvement of Reductive Dechlorination. J. Bacteriol. 180, 5159– 5164.
  • Rozie, H., Somers, W., Bonte, A., Visser, J., Van’t Riet, K. and Rombouts, F.M. (1988). Adsorption Characteristics of Endo-polygalacturonase Beads. Biotechnol. Appl. Bioc. 10, 346– 358. on Aljinate
  • Taspinar, A. and Kolankaya, N. (1998). Optimi- zation of enzymatic chlorine removal from Kraft pulp. B. Environ. Contam. Tox. 61, 15–21.
  • Thomas, D.R., Carswell, K.S. and Georgiou, G. (1992). Mineralization of biphenyl and PCBs by white rot fungus Phanerochaete chrysosporium. Biotechnol. Bioeng 40, 1395–1402.
  • Tuomela, M., Lyytikainen, M., Oivanena, P. and Hatakka, A., (1999). Mineralization and conversion of pentachlorophenol (PCP) in soil inoculated with the white-rot fungus Trametes versicolor . Soil Biol. Biochem 31, 65–74.
  • Ünal, A. and Kolankaya, N. (2004). Chlorin re- movel from pp’ DDT by laccase enzyme produced from Trametes versicolor. Turk- ish Electronic J. Biotechnol 2, 17–21.
  • Valentin, L., Feijooa, G., Moreirab, M.T. and Lema, J.M., (2006). Biodegradation of polycyclic aromatic hydrocarbons in for- est and salt marsh soils by white-rot fungi. Int. Biodeter. Biodegr 58, 15–21.
  • Walter, M., Boyd-Wilsona, K.S.H., McNaugh- tonb, D. and Northcott, G. (2005). Labo- ratory trials on the bioremediation of aged pentachlorophenol residues. Int. Biodeter. Biodegr 55, 121–130.
  • Yadav, S. and. Reddy, C.A. (1992). Non- involvement of lignin peroxidases and manganese trichlorophenoxyacetic acid degradation by Phanerochaete chrysosporium. Bio- technol. Lett 14, 1089–1092. in 2,4,5
  • Yadav, J.S., Ouensen, J.F., Tiedje, J.F.Q.J.M. and Reddy, C.A. (1995). Degradation of Polychlorinated Biphenyl Mixtures (Aro- clors 1242,1254, and 1260) by the White Rot Fungus Phanerochaete chrysospo- rium as Evidenced by Congener-Specific Analysis. Appl. Environ. Microbiol 2560– 2565.
  • Yarapolov, A.I., Skorobogatko, O.V., Vartanov, S.S. and Varfolomeyev, S.D. (1994). Lac- case properties, catalytic mechanism and applicability. Appl. Biochem. Biotech 49, 257–279.
  • Zouari, H., Labat, M. and Sayadi, S. (2002). Degradation of 4-chlorophenol by the white rot fungus Phanerochaete chryso- sporium in free and immobilized cultures. Bioresource Technol 84,145–150.
Toplam 32 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Bölüm Araştırma Makalesi
Yazarlar

Arzu Ünal Bu kişi benim

Ahmet Çabuk

Nazif Kolonkaya Bu kişi benim

Yayımlanma Tarihi 6 Temmuz 2011
Yayımlandığı Sayı Yıl 2011 Cilt: 1 Sayı: 2

Kaynak Göster

APA Ünal, A., Çabuk, A., & Kolonkaya, N. (2011). DECHLORINATION OF 2,4,6-TRICHLOROPHENOL BY FREE AND IMMOBILIZED LACCASE FROM TRAMETES VERSICOLOR IN A LAB SCALE BIOREACTOR. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology, 1(2), 113-24.
AMA Ünal A, Çabuk A, Kolonkaya N. DECHLORINATION OF 2,4,6-TRICHLOROPHENOL BY FREE AND IMMOBILIZED LACCASE FROM TRAMETES VERSICOLOR IN A LAB SCALE BIOREACTOR. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology. Temmuz 2011;1(2):113-24.
Chicago Ünal, Arzu, Ahmet Çabuk, ve Nazif Kolonkaya. “DECHLORINATION OF 2,4,6-TRICHLOROPHENOL BY FREE AND IMMOBILIZED LACCASE FROM TRAMETES VERSICOLOR IN A LAB SCALE BIOREACTOR”. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology 1, sy. 2 (Temmuz 2011): 113-24.
EndNote Ünal A, Çabuk A, Kolonkaya N (01 Temmuz 2011) DECHLORINATION OF 2,4,6-TRICHLOROPHENOL BY FREE AND IMMOBILIZED LACCASE FROM TRAMETES VERSICOLOR IN A LAB SCALE BIOREACTOR. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology 1 2 113–24.
IEEE A. Ünal, A. Çabuk, ve N. Kolonkaya, “DECHLORINATION OF 2,4,6-TRICHLOROPHENOL BY FREE AND IMMOBILIZED LACCASE FROM TRAMETES VERSICOLOR IN A LAB SCALE BIOREACTOR”, Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology, c. 1, sy. 2, ss. 113–24, 2011.
ISNAD Ünal, Arzu vd. “DECHLORINATION OF 2,4,6-TRICHLOROPHENOL BY FREE AND IMMOBILIZED LACCASE FROM TRAMETES VERSICOLOR IN A LAB SCALE BIOREACTOR”. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology 1/2 (Temmuz 2011), 113-24.
JAMA Ünal A, Çabuk A, Kolonkaya N. DECHLORINATION OF 2,4,6-TRICHLOROPHENOL BY FREE AND IMMOBILIZED LACCASE FROM TRAMETES VERSICOLOR IN A LAB SCALE BIOREACTOR. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology. 2011;1:113–24.
MLA Ünal, Arzu vd. “DECHLORINATION OF 2,4,6-TRICHLOROPHENOL BY FREE AND IMMOBILIZED LACCASE FROM TRAMETES VERSICOLOR IN A LAB SCALE BIOREACTOR”. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology, c. 1, sy. 2, 2011, ss. 113-24.
Vancouver Ünal A, Çabuk A, Kolonkaya N. DECHLORINATION OF 2,4,6-TRICHLOROPHENOL BY FREE AND IMMOBILIZED LACCASE FROM TRAMETES VERSICOLOR IN A LAB SCALE BIOREACTOR. Anadolu University Journal of Science and Technology C - Life Sciences and Biotechnology. 2011;1(2):113-24.