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FARKLI AĞIZ GARGALARININ ANTİBAKTERİYEL ETKİLERİNİN İN-VİTRO OLARAK İNCELENMESİ

Year 2019, Volume: 46 Issue: 1, 1 - 6, 01.04.2019

Abstract

Amaç: Bu in vitro çalışmada altı farklı ağız gargarasının Streptococcus mutans, Enterococcus faecalis, Bacillus subtilis, Lactobacillus casei, Staphylococus aureus üzerine antibakteriyel etkileri Agar Difüzyon Testi ile incelendi.
Gereç ve Yöntem: Farklı özellikteki ağız gargaralarının antibakteriyel etkilerini incelemek için agar plakalarının üzerine 5 mm genişliğinde 7 yuva açıldı. Her bir ağız gargarası bu yuvalara yerleştirildi ve bir tanesi kontrol grubu olarak boş bırakıldı. 24 saat sonra test edilen mikroorganizmalara karşı oluşan inhibisyon alanları ölçülerek değerlendirmeler yapıldı. Sonuçların istatistiksel analizi için Kruskal Wallis and Mann Whitney U Testi kullanıldı.
Bulgular: Test edilen ağız gargaralarının 5 farklı mikro-organizma üzerine antibakteriyel etkilerinin olduğu görüldü (P<0.05). Oluşturdukları inhibisyon zon kalınlıkları yönüyle en etkili olan preparatın altı numaralı preparat olduğu sonucuna varıldı. (P<0.05).
Sonuç: Çalışmamızda kullanılan preparatların anti bakteriyel özelliğe sahip oldukları in-vitro koşullarda gösterilmiş olsa da, klinik etkinliğin sağlanmasında ağız içi koşullar önemli rol oynar.

References

  • Shah S, Bargale S, Dave BH, Deshpande A, Kariya PB, Karri A. Comparison of Antimicrobial Efficacy of (between) 0.2% Chlorhexidine and Herbal Mouthwash on Salivary Streptococcus mutans: A Randomized Controlled Pilot Study. Contemp Clin Dent. 2018 Jul-Sep;9(3):440-445.
  • Pontoriero R, Tonelli MP, Carnevale G, Mombelli A, Nyman SR, Lang NP. Experimentally induced peri-implant mucositis. A clinical study in humans. Clin Oral Implants Res. 1994 Dec;5(4):254-9.
  • Lakade LS, Shah P, Shirol D. Comparison of antimicrobial efficacy of chlorhexidine and combination mouth rinse in reducing the mutans streptococcus count in plaque. J Indian Soc Pedod Prev Dent 2014;32:91-6.
  • Gupta N, Bhat M. Comparative evaluation of 0.2 percent chlorhexidine and magnetized water as a mouth rinse on Streptococcus mutans in children. Int J Clin Pediatr Dent 2011;4:190-4.
  • Nayak SS, Kumar BR, Ankola AV, Hebbal M. The efficacy of Terminalia chebula rinse on Streptococcus mutans count in saliva and its effect on salivary pH. Oral Health Prev Dent 2010;8:55-8.
  • Vinson L, Gilbert PR, Sanders BJ, Moser E, Gregory RL. Silver Diamine Fluoride and Potassium Iodide Disruption of In Vitro Streptococcus mutans Biofilm. J Dent Child (Chic). 2018 Sep 15;85(3):120-124.
  • Axelsson P, Lindhe J. Efficacy of mouthrinses in inhibiting dental plaque and gingivitis in man. J Clin Periodontol. 1987;14(4):205-12.
  • Charles CH, Sharma NC, Galustians HJ, Qaqish J, McGuire JA, Vincent JW. Comparative efficacy of an antiseptic mouthrinse and an antiplaque/antigingivitis dentifrice. A six-month clinical trial. Journal of the American Dental Association. 2001;132(5):670-5.
  • Alshehri FA. The use of mouthwash containing essential oils (LISTERINE®) to improve oral health: A systematic review. Saudi Dent J. 2018 Jan;30(1):2-6.
  • Sharma A, Agarwal N, Anand A, Jabin Z. To compare the effectiveness of different mouthrinses on Streptococcus mutans count in caries active children. J Oral Biol Craniofac Res. 2018 May-Aug;8(2):113-117.
  • Aktas A, Giray B. Diş Hekimliğinde Klorheksidin: Özellikleri ve Güncel Kullanım Alanları. Turkiye Klinikleri J Dental Sci. 2010;16(1):51-8.
  • Moran J, Addy M, Newcombe R. A clinical trial to assess the efficacy of sanguinarinezinc mouthrinse (Veadent) compared with chlorhexidine mouthrinse (Corsodyl). J Clin Periodontol. 1988;15(10):612-6.
  • Croaker A, King GJ, Pyne JH, AnoopkumarDukie S, Simanek V, Liu L. Carcinogenic potential of sanguinarine, a phytochemical used in 'therapeutic' black salve and mouthwash. Mutat Res. 2017 Oct;774:46-56.
  • Croaker A, King GJ, Pyne JH, AnoopkumarDukie S, Liu L. Sanguinaria canadensis: Traditional Medicine, Phytochemical Composition, Biological Activities and Current Uses. Int J Mol Sci. 2016; 27;17(9).

Investigation of Antibacterial Effects of Different Mouth Rinses: An In-Vitro Study

Year 2019, Volume: 46 Issue: 1, 1 - 6, 01.04.2019

Abstract

Aim: In this in vitro study six different mouth rinses were tested for their antimicrobial activity against five oral pathogens which is Streptococcus mutans, Enterococcus faecalis, Bacillus
subtilis, Lactobacillus casei, Staphylococus aureus by well Agar Diffusion Test. Material and Methods: For the evaluation of antibacterial properties of materials, 7 holes prepared by 5 mm diameter on each agar plate. Different 6 mouth rinses insert in this holes but one of them lived empty for control. Inhibition zones of against the test organism measured after 24 hours. Kruskal Wallis and Mann Whitney U Test were used for evaluate of the findings by statistically. Results: According to 24 h results all tested mouth rinses have antimicrobial activity on tested
5 different organism type (P<0,05). The most effective mouth rinse was number six according inhibition zones of against the test organism. (P<0,05).  

References

  • Shah S, Bargale S, Dave BH, Deshpande A, Kariya PB, Karri A. Comparison of Antimicrobial Efficacy of (between) 0.2% Chlorhexidine and Herbal Mouthwash on Salivary Streptococcus mutans: A Randomized Controlled Pilot Study. Contemp Clin Dent. 2018 Jul-Sep;9(3):440-445.
  • Pontoriero R, Tonelli MP, Carnevale G, Mombelli A, Nyman SR, Lang NP. Experimentally induced peri-implant mucositis. A clinical study in humans. Clin Oral Implants Res. 1994 Dec;5(4):254-9.
  • Lakade LS, Shah P, Shirol D. Comparison of antimicrobial efficacy of chlorhexidine and combination mouth rinse in reducing the mutans streptococcus count in plaque. J Indian Soc Pedod Prev Dent 2014;32:91-6.
  • Gupta N, Bhat M. Comparative evaluation of 0.2 percent chlorhexidine and magnetized water as a mouth rinse on Streptococcus mutans in children. Int J Clin Pediatr Dent 2011;4:190-4.
  • Nayak SS, Kumar BR, Ankola AV, Hebbal M. The efficacy of Terminalia chebula rinse on Streptococcus mutans count in saliva and its effect on salivary pH. Oral Health Prev Dent 2010;8:55-8.
  • Vinson L, Gilbert PR, Sanders BJ, Moser E, Gregory RL. Silver Diamine Fluoride and Potassium Iodide Disruption of In Vitro Streptococcus mutans Biofilm. J Dent Child (Chic). 2018 Sep 15;85(3):120-124.
  • Axelsson P, Lindhe J. Efficacy of mouthrinses in inhibiting dental plaque and gingivitis in man. J Clin Periodontol. 1987;14(4):205-12.
  • Charles CH, Sharma NC, Galustians HJ, Qaqish J, McGuire JA, Vincent JW. Comparative efficacy of an antiseptic mouthrinse and an antiplaque/antigingivitis dentifrice. A six-month clinical trial. Journal of the American Dental Association. 2001;132(5):670-5.
  • Alshehri FA. The use of mouthwash containing essential oils (LISTERINE®) to improve oral health: A systematic review. Saudi Dent J. 2018 Jan;30(1):2-6.
  • Sharma A, Agarwal N, Anand A, Jabin Z. To compare the effectiveness of different mouthrinses on Streptococcus mutans count in caries active children. J Oral Biol Craniofac Res. 2018 May-Aug;8(2):113-117.
  • Aktas A, Giray B. Diş Hekimliğinde Klorheksidin: Özellikleri ve Güncel Kullanım Alanları. Turkiye Klinikleri J Dental Sci. 2010;16(1):51-8.
  • Moran J, Addy M, Newcombe R. A clinical trial to assess the efficacy of sanguinarinezinc mouthrinse (Veadent) compared with chlorhexidine mouthrinse (Corsodyl). J Clin Periodontol. 1988;15(10):612-6.
  • Croaker A, King GJ, Pyne JH, AnoopkumarDukie S, Simanek V, Liu L. Carcinogenic potential of sanguinarine, a phytochemical used in 'therapeutic' black salve and mouthwash. Mutat Res. 2017 Oct;774:46-56.
  • Croaker A, King GJ, Pyne JH, AnoopkumarDukie S, Liu L. Sanguinaria canadensis: Traditional Medicine, Phytochemical Composition, Biological Activities and Current Uses. Int J Mol Sci. 2016; 27;17(9).
There are 14 citations in total.

Details

Primary Language Turkish
Subjects Dentistry
Journal Section Research Article
Authors

Yıldırım Hakan Bağış This is me

Nilsun Bağış

Publication Date April 1, 2019
Published in Issue Year 2019 Volume: 46 Issue: 1

Cite

APA Bağış, Y. H., & Bağış, N. (2019). FARKLI AĞIZ GARGALARININ ANTİBAKTERİYEL ETKİLERİNİN İN-VİTRO OLARAK İNCELENMESİ. Ankara Üniversitesi Diş Hekimliği Fakültesi Dergisi, 46(1), 1-6.
AMA Bağış YH, Bağış N. FARKLI AĞIZ GARGALARININ ANTİBAKTERİYEL ETKİLERİNİN İN-VİTRO OLARAK İNCELENMESİ. AÜDHF dergisi. April 2019;46(1):1-6.
Chicago Bağış, Yıldırım Hakan, and Nilsun Bağış. “FARKLI AĞIZ GARGALARININ ANTİBAKTERİYEL ETKİLERİNİN İN-VİTRO OLARAK İNCELENMESİ”. Ankara Üniversitesi Diş Hekimliği Fakültesi Dergisi 46, no. 1 (April 2019): 1-6.
EndNote Bağış YH, Bağış N (April 1, 2019) FARKLI AĞIZ GARGALARININ ANTİBAKTERİYEL ETKİLERİNİN İN-VİTRO OLARAK İNCELENMESİ. Ankara Üniversitesi Diş Hekimliği Fakültesi Dergisi 46 1 1–6.
IEEE Y. H. Bağış and N. Bağış, “FARKLI AĞIZ GARGALARININ ANTİBAKTERİYEL ETKİLERİNİN İN-VİTRO OLARAK İNCELENMESİ”, AÜDHF dergisi, vol. 46, no. 1, pp. 1–6, 2019.
ISNAD Bağış, Yıldırım Hakan - Bağış, Nilsun. “FARKLI AĞIZ GARGALARININ ANTİBAKTERİYEL ETKİLERİNİN İN-VİTRO OLARAK İNCELENMESİ”. Ankara Üniversitesi Diş Hekimliği Fakültesi Dergisi 46/1 (April 2019), 1-6.
JAMA Bağış YH, Bağış N. FARKLI AĞIZ GARGALARININ ANTİBAKTERİYEL ETKİLERİNİN İN-VİTRO OLARAK İNCELENMESİ. AÜDHF dergisi. 2019;46:1–6.
MLA Bağış, Yıldırım Hakan and Nilsun Bağış. “FARKLI AĞIZ GARGALARININ ANTİBAKTERİYEL ETKİLERİNİN İN-VİTRO OLARAK İNCELENMESİ”. Ankara Üniversitesi Diş Hekimliği Fakültesi Dergisi, vol. 46, no. 1, 2019, pp. 1-6.
Vancouver Bağış YH, Bağış N. FARKLI AĞIZ GARGALARININ ANTİBAKTERİYEL ETKİLERİNİN İN-VİTRO OLARAK İNCELENMESİ. AÜDHF dergisi. 2019;46(1):1-6.