Deksametason Kullanıldığında Myoblastlar Yüzeyindeki Değişikliklerin Scanning Elektron Mikroskobu İle İncelenmesi

Abstract

Hücreler fonksiyonlarına bağlı bazı yüzey özelliklerine sahiptir.Metabolizması değişen hücrelerin yüzeyinde de bazı değişiklikler beklenir.Bu çalışmada fareden elde edilen N-ras Onkogeni bağlanmış myoblastlar değişik kültür ortamlarında üretildi. Ortama deksametazon eklenerek değişik kültür ortamının ve dekzametazonun etkileri sonucu hücrelerin yüzeylerindeki değişiklikler scanning Elektron Mikroskop ile incelendi. Değişik kültür ortamında, üretilen hücrelerin yüzeylerinde bazı farklılıklar tespit edildi. Ortama deksametazon eklenmesinden sonra hücrelerin yüzeylerinde mikrovilli artışı yanı sıra düzensiz kıvrıntı, kabartı, katlantı gibi deformasyon özellikeri tespit edildi.

Keywords

• Elektron Mikroskop
• deformasyon
• deksametazon

EXAMINATION OF CHANGES ON THE SURFACE OF MYOBLASTS WHEN DEXAMETHASONE IS USED BY SCANNING ELECTRON MICROSCOPE

Abstract

Cells have certain surface characteristics depending on their functions, When the metabolism of the cells changes, some differences on their surfaces are also expected. In this study, the myoblasts, given N-ras oncogen were produced in different cultur media, Dexamethasone was added to the medium and the effects ol media and dexamethasone on the surfaces of these cells were examined by a scanning electron microscopy. Certain differentiations were determined on the surface of cells grown in different cultur media. The increases of microvilli and some deformalion characteristics such as rufiling and swelling were determined after adding dexamethasone to the cultur medium.

Keywords

• deksametazon
• deformation
• ELECTRON MICROSCOPE

References

1. 1. Americen type culture collection ;: Media hand book; cell culture media and reagent cell culture media formulations, American type culture collection catalogues, 1984.
2. 2. Bar-Segi D and Feramisco JR : Induction of membrana rufiling and İluid-phase pinocytosis in guiescant fibroblast by ras proteins, Science, 233 : 1061-1068, 1986.
3. 3. Chardin P Touchot N Zaahraoni A Pizon V Lerosey I Olofsson B and Tavitian A ; Structüre and organization of the ras gene family in human, Proceeding of a NATO advanced research workshop on ras oncogenes, Plenum Press, Newyork, November : 1-9, 1989,
4. 4. Freshney RI : Culture of animal cells a manual of basis technigue, Second Editon, Alan R., Liss Inc., Newyork, 1987.
5. 5. Gossett LA Zang W and Olson EN : Dexamethosone depend inhibition of differantiation of C2 myoblasts bearing steroid- inducible N-ras oncogenes, Journal of Cell Biology, 106 : 2127-2137, 1988.
6. 8. Hall A Morris JDH Price B (Hoyd A Hancock JF Gardener 5 Houslay MD Wakelam MJO and Marshali CJ : The function of the mammalian ras proteins. Proceeding of the NATO advanced research workshop on ras oncogenes, Plenum Press, Newyork, November, 1-0, 1989.
7. 7. Linstead P Jennings B Presscott A Hawley P Wam A and Gibson I : Scanning electron microscopy and ihe transformed phenotype, Micron and Microscopica Acta, 19 (3) : 155-162, 1988.
8. 8. Weiss L : Cell and tissue biology, A texbook of histology, Sixth edition, Urban and Schwerzenberg, İnc., Baltimore, Munich, 1988,

9. 9. Yaffe D and Saxel O. : Serial passaging and differentional of myogenic cell isolated from dystrophic mouse muscle, Nature, 270 : 725-727, 1977.