Histological and Histochemical Investigation in Lung

Year 1981, Volume: 34 Issue: 4, 681 - 688, 31.12.1981

Ergin Açıkalın

Abstract

Bu çalışmada erişkin sıçan akciğerinde belirli epitel hücrelerinin histolojik ve histokimyasal özellikleri PAS, Kurşunlu Hematoksilin, Toluidin Mavisi (asit hidroliz sonrası), Masson-Fontana ve Singh arjirofil yöntemleriyle incelenmiştir. Tip I ve tip II pnömositler, Clara hücreleri ve makrofajlar; boyanma farklılıklarına göre ayırt edilmiştir. Tip II hücreler ve makrofajlarda PAS pozitif granüller görülmüştür. Bronşial ve alveoler epitelde arjirofil granüller içeren bazı hücreler endokrin benzeri davranış göstermektedir. Kurşunlu Hematoksilin ve Toluidin Mavisi ile protein yapılı granüller doğrulanmıştır. Sonuçlar akciğer epitelinde APUD tipi endokrin hücrelerin varlığını desteklemektedir.

Keywords

alveol epiteli , Clara hücreleri , tip II pnömosit , arjirofil granüller , APUD sistemi , histokimya

Akciğerde Histolojik ve Histokimyasal İnceleme

Abstract

This study explores the histological and histochemical characteristics of specific epithelial cell types in adult rat lungs using PAS, lead hematoxylin, toluidine blue (post-acid hydrolysis), Masson-Fontana, and Singh argyrophil methods. Type I and type II pneumocytes, Clara cells, and macrophages were identified and distinguished through their staining patterns. PAS-positive granules were found in type II cells and macrophages. Argyrophil granules were present in some cells of both the bronchial and alveolar epithelium, suggesting endocrine-like behavior. Lead hematoxylin and toluidine blue confirmed protein-rich granules potentially associated with polypeptide hormone production. These findings support the presence of APUD-type endocrine cells in pulmonary epithelium.

Keywords

alveolar epithelium , Clara cells , type II pneumocytes , argyrophil granules , APUD system , histochemistry

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