Laktat Dehidrogenaz (LDH) İzoenzimlerinin Sellüloz Asetat Elektroforezi ile Ayırt Edilmeleri
Abstract
This study investigates the separation of LDH isoenzymes using cellulose acetate electrophoresis. The electrophoresis method recommended by Gelman was used in the study; however, due to insufficient results, various technical modifications were made. The waiting time for cellulose acetate strip preparation was reduced, the ratio of methanol to acetic acid was changed for strip cleaning, and the drying process was carried out at room temperature.
In tests performed with 13 normal serum samples, the isoenzyme ratios obtained differed from the values reported by Gelman. This difference was determined to be due specifically to the solution used for strip cleaning. Furthermore, LDH isoenzyme percentages from different sources were compared to highlight the impact of technical incompatibilities on the results.
Consequently, these modifications shortened the application time, simplified the method, and yielded reliable results in LDH isoenzyme determination.
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References
- 1 - Gelman procedures, Technic for electrophoresis. Gelman Instrument Company, 15–35, 1968.
- 2 - Opher, C., Albert, W., Collier and Joseph, M. Miller: A rapid electrophoretic method for the determination of the isoenzymes of serum lactate dehydrogenase. Clin. Chem., 12: 308–313, 1966.
- 3 - Papadopulos, N., Kintzios, J. A.: Quantitative electrophoretic determination of LDH isoenzymes. Am. J. Clin. Pathol., 47: 96–99, 1967.
- 4 - Schmidt, D. M., Black, M. B., Miller, J. H., Chinn, D. J.: Modified microzone cellulose acetate method for lactic dehydrogenase isoenzymes—normal values. Am. J. Med. Technol., 35: 53–157, 1969.
- 5 - Vander, H. J., Zondag, H. A., vander, K. M. W.: LDH isoenzymes in myocardial infarction. Clin. Chim. Acta, 7: 540–544, 1962.