Safkan Bir Atın Lokal Polyester Reçine ile Kesitli Böbrek Plastinasyonunun Anatomik Değerlendirmesi ve Hazırlama Prosedürü

Abstract

Bu çalışma, lokal polyester reçine ile böbreğin ince kesit plastinasyonu için bir protokol geliştirmeyi ve örneğin anatomik detaylarını incelemeyi amaçlamıştır. Örnek %10 formalin fiksasyon solüsyonu ile sabitlendi ve ardından 3 mm'lik dilimlere ayrıldı. Polyester plastinasyon tekniğinin dört aşaması kullanıldı. İlk olarak örnekler dehidrasyon için -25ºC'de %99,5 aseton banyosunda tutulmuştur. Bu işlemden sonra kesitler polyester reçineye yerleştirildi ve vakum altında oda sıcaklığında (20ºC) impregnasyon işlemine başlandı. Zorlu impregnasyon işleminden sonra kürleme odaları oluşturuldu ve kürleme işlemine ultraviyole ışık altında devam edildi. Uygulanan her aşamanın verileri dikkatlice kaydedildi ve böbrek bölümlerinin polyester plastinasyonu için protokol başarıyla oluşturuldu. Örnekler, ince bir kesit görünümü sayesinde farklı bir bakış açısıyla gözlemlenmiştir. Örneklerin anatomik morfolojisi ve böbreğin kesit bölümlerine ait yapılar, böbrek paraşimi ve dolaşım bileşenleri gibi, iyi korunmuştur. Elde edilen son ürünler, böbreklerin kesitsel anatomik çalışmaları için iyi birer eğitim materyalleri olarak kullanılabileceği düşünülmektedir.

Keywords

• At
• Böbrek morfololojisi
• polyester plastinasyon
• kesit anatomisi

Anatomical evaluation and preparation procedure of a cross-sectioned kidney plastination of a thoroughbred horse with local polyester resin

Abstract

This study aimed to develop a protocol for the thin section plastination of the kidney with local polyester resin and examine the anatomical details of this specimen. The sample was fixed with 10% formalin fixation solution and then sectioned into 3 mm slices. The four stages of the polyester plastination technique were used. Firstly, the samples were kept in 99.5% acetone baths at -25ºC for the dehydration. After this process, the sections were placed in polyester resin and the impregnation process was started at room temperature (20ºC) under vacuum. Following the forced impregnation, the curing chambers were constructed and the curing process was continued under ultraviolet light. The data of each applied stage were recorded carefully and the protocol for polyester plastination of kidney sections was successfully established. The specimens were observed from a different point of view with a thin cross-sectional appearance. The anatomical morphology and the structures of the sections of the kidney such as renal parenchyma and circulatory components were preserved well. The final products could be used as educational samples for cross-sectional anatomical training of kidneys.

Keywords

• Horse
• kidney morphology
• polyester plastination
• sectional anatomy

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