Blood Macro (Ca, K, Mg, Na, P) and Micro (Al, B, Mo, Se, Mn, Sb, Sn, Tl) Element Status and Correlations in Shelter Dogs

Abstract

Depending on bioconcentration, elements are classified as macro and micro. While some are essential for biological processes but may be toxic above the tolerable concentrations, than is necessary to monitor the concentrations. Also, the deficiency or excess of micro- and macroelements may diminish the effect of substances, such as other elements or drugs, or cause them to exert toxic effects. In the present study, macro (Ca, K, Mg, Na, and P) and micro (Al, B, Mn, Mo, Sb, Sn, and Tl) element concentrations and correlations were determined in shelter dogs. According to the results, the high to low micro- and macroelement concentrations were found to be K>Ca>P>Mg>Na and Se>Al>Mn>B>Mo>Sb>Sn>Tl respectively. Statistically significant positive (Ca-Sn, Mg-Mn, Mg-Sb, Mg-Se, P-Al, P-Sb) and negative (K-Al, K-Mn, Na-Al, Na-Sb, Na-Sn) correlations were determined between macro- and microelements. The concentrations of the macroelements that were analyzed in this study were within the reference range. There is no data on the concentrations and biological or toxic effects of some elements, such as B, Sb, Sn, and Tl, in dogs. Reference biomarkers should be identified for the early diagnosis of conditions induced by altered element concentrations and the health impacts of these changes should be thoroughly investigated.

Keywords

• Correlation
• Dogs Blood
• ICP Analysis
• Macroelements
• Microelements

References

1. Abdelnour SA, Abd El-Hack, ME, Swelum, A, et al (2018): The vital roles of boron in animal health and production: A comprehensive review. J Trace Elem Med Biol, 50, 296-304.
2. Adeyemi JO, Onwudiwe DC (2020): Chemistry and some biological potential of bismuth and antimony dithiocarbamate complexes. Molecules, 25, 305.
3. Ahmed MH, Wilkens, MR, Ganter, M, et al (2021): Serum parameters related to mineral homeostasis and energy metabolism in ewes kept on different dietary magnesium supply during the transition period. Res Vet Sci, 134, 19-26
4. Ali H, Khan E (2018): What are heavy metals? Long-standing controversy over the scientific use of the term ‘heavy metals’–proposal of a comprehensive definition. Toxicol Environ Chem, 100, 6-19.
5. Altınok Yipel, F, Yipel, M (2024): Comparison of serum and plasma aluminum concentrations in sheep. REPVAS, 1(2), 64-72.
6. Altunok V, Yazar E, Yuksek N (2007): Selected blood serum elements in Van (Turkey) Cats. Acta Vet Brno, 76, 171–177.
7. Badea E, Goran, GV, ȚOCA, C et al (2018): Assessment of heavy metal and mineral levels in hair samples from dogs with mammary neoplasms. Bull UASVM Food Sci Tech., 75, 1.
8. Badea E, Goran GV, Crivineanu V (2019): Aluminum levels in cats and dogs. Sci Works C Vet Med, 65, 21-23.

9. Cedeño Y, Miranda, M, Orjales, I, Herrero-Latorre, C et al (2020): Trace Element Levels in Serum Are Potentially Valuable Diagnostic Markers in Dogs. Anim,10, 2316.
10. Cedeño Y, Miranda Castañón, MI, Orjales Galdo, I, et al (2020): Serum Concentrations of Essential Trace and Toxic Elements in Healthy and Disease-Affected Dogs. Anim, 10, 1052.
11. Davies M, West, J, Williams, C, et al (2017): Mineral status in canine medial coronoid process disease: a cohort study using analysis of hair by mass spectrometry. Vet Rec, 180, 448-448.
12. Duydu Y, Başaran, N, Yalçın, CÖ, et al (2019): Boron-exposed male workers in Turkey: no change in sperm Y: X chromosome ratio and in offspring’s sex ratio. Arch Toxicol, 93, 743-751.
13. Fielder SE (2015): Merck Co, Inc, Kenilworth, NJ, USA.
14. Gonzalez-Reyes RE, Gutierrez-Alvarez AM, Moreno CB (2007): Manganese and epilepsy: a systematic review of the literature. Brain Res Rev, 53, 332–336.
15. Gupta RC (2012): Veterinary Toxicology: Basic and Clinical Principles, 2nd Ed. Academic Press, USA.
16. Hashmi GM, Shah MH (2012): Comparative assessment of essential and toxic metals in the blood of rheumatoid arthritis patients and healthy subjects. Biol Trace Elem Res, 146, 13-22.
17. Hinwood AL, Stasinska, A, Callan, AC et al (2015): Maternal exposure to alkali, alkali earth, transition and other metals: concentrations and predictors of exposure. Env Pollut, 204, 256-263.
18. Kaneko JJ, Harvey JW, Bruss ML (2008): Clinical biochemistry of domestic animals. Academic press.
19. Kilpatrick S, Jacinto, A, Foale, RD et al (2014): Whole blood manganese concentrations in dogs with primary hepatitis. J Small Anim Pract, 55, 241–246.
20. Komarova T, McKeating D, Perkins, AV et al (2021): Trace Element Analysis in Whole Blood and Plasma for Reference Levels in a Selected Queensland Population, Australia. IJERPH, 18, 2652.
21. Kosla T, Skibniewska EM (2010): The content of aluminum in the hair of Yorkshire terrier dogs from the Warsaw area depending on sex, age and keeping conditions. Trace Elem Electroly, 27, 209–213.
22. Laur N, Kinscherf R, Pomytkin K, et al (2020): ICP-MS trace element analysis in serum and whole blood. PloS one, 15, e0233357.
23. Mert H, Mert N, Dogan I, et al (2008): Element status in different breeds of dogs. Biol Trace Elem Res, 125, 154-159.
24. Miller KBJS, Caton, JS, Schafer, DM, et al (2000): High dietary manganese lowers heart magnesium in pigs fed a low-magnesium diet. J Nutr, 130, 2032–2035.
25. Nordberg GF, Fowler BA, Nordberg M (2015): Handbook on the Toxicology of Metals. Academic press, USA.
26. NRC (2005): National Research Council. Mineral Tolerance of Animals, 2nd Ed. The National Academies Press, USA.
27. Pandey G, Madhuri S (2014): Heavy metals causing toxicity in animals and fishes. Res J Anim Vet Fishery Sci, 2, 17-23.
28. Park S, Lee M, Kim S (2005): Studies on the concentrations of Cd, Pb, Hg and Cr in dog serum in Korea. Asian Australas J Anim Sci, 18, 1623-1627.
29. Patrashkov SA, Petukhov, VL, Korotkevich OS, et al (2003): Content of heavy metals in the hair. J Phys IV, 107, 1025-1027.
30. Pilarczyk R, Wójcik, J, Czerniak, P, et al (2013): Concentrations of toxic heavy metals and trace elements in raw milk of Simmental and Holstein-Friesian cows from organic farm. Environ Monit Assess, 185, 8383-8392.
31. Plumlee K (2003): Clinical Veterinary Toxicology-E-Book. Elsevier Health Sciences.
32. Prashanth L, Kattapagari, KK, Chitturi RT, et al (2015): A review on role of essential trace elements in health and disease. J Dr NTR Univ Health Sci, 4, 75.
33. Schultze B, Lind PM, Larsson A, et al (2014): Whole blood and serum concentrations of metals in a Swedish population-based sample. Scand J Clin Lab, 74, 143-148.
34. Sgorlon S, Mattiello, A, Ronutti L, et al (2019): Concentration of elements in the hair of growing and adult dogs. Ital J Anim Sci, 18, 1126–1134.
35. Tatara Marcin R, Łuszczewska-Sierakowska I, Krupski W (2018): Serum concentration of macro-, micro-, and trace elements in Silver fox (Vulpes vulpes) and their interrelationships with morphometric, densitometric, and mechanical properties of the mandible. Biol Trace Elem Res, 185, 98-105.
36. Tiwari RM, Sinha M (2010): Veterinary Toxicology. Oxford Book Company, Delhi, India.
37. Tomza-Marciniak A, Pilarczyk, B, Bąkowska, M, et al (2012): Lead, cadmium and other metals in serum of pet dogs from an urban area of NW Poland. Biol Trace Elem Res, 149, 345-351.
38. Uluisik I, Karakaya HC, Koc A (2018): The importance of boron in biological systems. J Trace Elem Med Biol, 45, 156-162.
39. Vitale S, Hague, DW, Foss, K, et al (2019): Comparison of Serum Trace Nutrient Concentrations in Epileptics Compared to Healthy Dogs. Front Vet Sci, 6, 467.
40. Zaccaroni A, Corteggio A, Altamura, G et al (2014): Elements levels in dogs from “triangle of death” and different areas of Campania region (Italy). Chemosphere, 62-69.
41. Zemrani B, Bines JE (2020): Recent insights into trace element deficiencies: Causes, recognition and correction. Curr Opin Gastroenterol, 36, 110–117.
42. Zhou Q, Tanaka YK, Suzuki N, et al (2019): Species difference in antimony and arsenic metabolism between hamster and rat after administration of tri-or pentavalent inorganic antimony. Fundam Toxicol Sci, 6, 181-185.
43. Zioła A, Frankowski M, Siepak J (2008): Aluminium toxicity: fact or myth? Laboratorium, 3, 56–61.
44. Zoroddu MA, Aaseth J, Crisponi G et al (2019): The essential metals for humans: a brief overview. J Inorg Biochem, 195, 120-129.