Time-restricted feeding confers organ-specific protection against oxidative stress and inflammation

Özkan Duru; Elisha Akanbong; Ali Şenol; Haceli Öcal; Safiye Arzik; Miyase Çınar

doi:10.33988/auvfd.1885186

Time-restricted feeding confers organ-specific protection against oxidative stress and inflammation

Abstract

Intermittent fasting (IF), particularly time-restricted feeding (TRF), has garnered attention for its potential health benefits and organ protection mechanisms. This study investigated the effects of TRF on oxidative stress and inflammation in the liver and kidneys of Wistar albino rats. A total of 48 male rats were employed, divided into four groups: Group 1 had unrestricted access to food, Group 2 was fed one meal a day (23 hours of fasting and 1 hour of eating), Group 3 was fed two meals a day (18 hours of fasting, with each meal lasting 1 hour within a 6-hour window), and Group 4 was fed two meals a day (14 hours of fasting, with each meal lasting 1 hour within a 10-hour window). Over a 90-day trial, we assessed TAC, TOC, OSI, MDA and NO in liver and kidney tissues, and measured pro-inflammatory cytokines (IL-1β, IL-6, TNF-α) and the BCL-2/BAX ratio to evaluate apoptosis. The results demonstrated modulation of oxidative stress and inflammatory markers, with TRF reducing TOC and OSI values. Notably, TRF enhanced TAC levels and reduced levels of pro-inflammatory cytokines. The BCL-2/BAX ratio indicated a decrease in apoptosis in the TRF groups compared to group 1. Additionally, Group 4 appeared to be the most effective regimen. These findings highlight the potential of TRF to mitigate oxidative stress and inflammation, and protect the liver and kidneys. This research supports TRF's role in enhancing metabolic health and underscores the need for further studies to evaluate on different organs.

Keywords

Project Number

125O493

References

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Details

Primary Language

English

Subjects

Veterinary Biochemistry

Journal Section

Research Article

Authors

Özkan Duru ^*
0000-0002-8792-442X
Türkiye

Elisha Akanbong
0000-0002-2556-7236
Ghana

Ali Şenol
0000-0003-4080-7776
Türkiye

Haceli Öcal This is me
0009-0002-5437-8634
Türkiye

Safiye Arzik This is me
0000-0003-0872-9739
Türkiye

Miyase Çınar
0000-0003-3806-9938
Türkiye

Early Pub Date

August 19, 2026

Publication Date

-

Submission Date

February 16, 2026

Acceptance Date

July 2, 2026

Published in Issue

Year 2026 Number: Advanced Online Publication

DOI

https://doi.org/10.33988/auvfd.1885186

IZ

https://izlik.org/JA35CC57FE

Cite

RIS / Bibtex

APA

Duru, Ö., Akanbong, E., Şenol, A., Öcal, H., Arzik, S., & Çınar, M. (2026). Time-restricted feeding confers organ-specific protection against oxidative stress and inflammation. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Advanced Online Publication. https://doi.org/10.33988/auvfd.1885186

AMA

1.Duru Ö, Akanbong E, Şenol A, Öcal H, Arzik S, Çınar M. Time-restricted feeding confers organ-specific protection against oxidative stress and inflammation. Ankara Univ Vet Fak Derg. 2026;(Advanced Online Publication). doi:10.33988/auvfd.1885186

Chicago

Duru, Özkan, Elisha Akanbong, Ali Şenol, Haceli Öcal, Safiye Arzik, and Miyase Çınar. 2026. “Time-Restricted Feeding Confers Organ-Specific Protection Against Oxidative Stress and Inflammation”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, no. Advanced Online Publication. https://doi.org/10.33988/auvfd.1885186.

EndNote

Duru Ö, Akanbong E, Şenol A, Öcal H, Arzik S, Çınar M (August 1, 2026) Time-restricted feeding confers organ-specific protection against oxidative stress and inflammation. Ankara Üniversitesi Veteriner Fakültesi Dergisi Advanced Online Publication

IEEE

[1]Ö. Duru, E. Akanbong, A. Şenol, H. Öcal, S. Arzik, and M. Çınar, “Time-restricted feeding confers organ-specific protection against oxidative stress and inflammation”, Ankara Univ Vet Fak Derg, no. Advanced Online Publication, Aug. 2026, doi: 10.33988/auvfd.1885186.

ISNAD

Duru, Özkan - Akanbong, Elisha - Şenol, Ali - Öcal, Haceli - Arzik, Safiye - Çınar, Miyase. “Time-Restricted Feeding Confers Organ-Specific Protection Against Oxidative Stress and Inflammation”. Ankara Üniversitesi Veteriner Fakültesi Dergisi. Advanced Online Publication (August 1, 2026). https://doi.org/10.33988/auvfd.1885186.

JAMA

1.Duru Ö, Akanbong E, Şenol A, Öcal H, Arzik S, Çınar M. Time-restricted feeding confers organ-specific protection against oxidative stress and inflammation. Ankara Univ Vet Fak Derg. 2026. doi:10.33988/auvfd.1885186.

MLA

Duru, Özkan, et al. “Time-Restricted Feeding Confers Organ-Specific Protection Against Oxidative Stress and Inflammation”. Ankara Üniversitesi Veteriner Fakültesi Dergisi, no. Advanced Online Publication, Aug. 2026, doi:10.33988/auvfd.1885186.

Vancouver

1.Özkan Duru, Elisha Akanbong, Ali Şenol, Haceli Öcal, Safiye Arzik, Miyase Çınar. Time-restricted feeding confers organ-specific protection against oxidative stress and inflammation. Ankara Univ Vet Fak Derg. 2026 Aug. 1;(Advanced Online Publication). doi:10.33988/auvfd.1885186