Streptozotosin ile indüklenmiş diyabetik sıçanlarda zeytin yaprağı ekstraktının sindirim enzimi inhibisyonuna ve insülin üretimine etkisi

Abstract

Zeytin yaprağı potansiyel olarak sağlık üzerine yararlı etkileri olduğu düşünülen başlıca oleuropein olmak üzere biyoaktif bileşenlere sahiptir. Bu çalışma, streptozotosin (STZ) ile indüklenmiş diyabetik sıçanlarda zetin yaprağı ekstraktının (OLE) in vivo karbonhidrat sindirim enzimleri inhibisyonunun ve pankreasta insülin mevcudiyetinin araştırılmasını amaçlamaktadır. Deney sonunda kan glukoz seviyeleri, insülin seviyeleri, glikozillenmiş hemoglobin (HbA1c), α-amilaz ve α-glukozidaz aktiviteleri analizi ile immunohistokimyasal çalışma yapıldı. OLE tedavi grubunda kan glukoz seviyeleri ve HbA1c anlamlı şekilde azalırken insülin seviyeleri arttı. Bunun yanında OLE, Akarboz grubuna göre dikkat çekici şekilde α-amilaz ve α-glukozidaz aktivitelerinde inhibitör etki gösterdi. Immunohistokimyasal analizde OLE’nin β-hücrelerinde insülin için kısmi pozitif immunoreaksiyon gösterdiği gözlendi. OLE akarboz ile karşılaştırıldığında sindirim sistemi bakımından daha tolere edilebilir olduğu düşünüldüğünde antidiyabetik ilaçlara göre daha iyi bir fitoformülasyon olabilir. OLE, diyabet tedavisi için ek bir faydalı etki sunabilir.

Keywords

• α-amilaz,α-glukozidaz,hipoglisemi,immunohistokimya,zeytin yaprağı

The effect of olive leaf extract on digestive enzyme inhibition and insulin production in streptozotocin-induced diabetic rats

Abstract

Olive leaf has natural bioactive compounds, mainly oleuropein, that are widely considered to have potentially beneficial effects on health. This study aimed to evaluate the effects of olive leaf extract (OLE) on the inhibition of carbohydrate digestive enzymes, and immunohistochemical study of insulin in the pancreas of in vivo streptozotocin-induced diabetic rats. Blood glucose levels, insulin, glycated hemoglobin (HbA1c), α-amylase and α-glucosidase activities, and an immunohistochemical study were performed at the end of the experiment. In the OLE treated group, blood glucose levels and HbA1c significantly decreased while insulin levels increased. Besides this, OLE treated group showed remarkable inhibitory activities on α-amylase and α-glucosidase compared with the Acarbose treated group. It was observed that OLE exhibited partial positive immunoreaction for insulin in β-cells through immunohistochemical analysis. Considering that OLE is more tolerable for digestion system compared to acarbose, it may be a better fitoformulation for antidiabetic medications. OLE could offer an additional beneficial effect for the treatment of diabetes.

Keywords

• hypoglycemic,α-amylase,α-glucosidase,immunohistochemistry,olive leaf

References

1. 1. Harvey RA, Ferrier DR. (2011): Lippincott’s Illustrated Reviews: Biochemistry. 337. In: RA Harvey (Ed), Integration of Metabolism: Diabetes Mellitus. 5th ed. Lippincott Williams & Wilkins, Hong Kong.
2. 2. Guo S. (2014): Insulin signaling, resistance, and metabolic syndrome: insights from mouse models into disease mechanisms. J Endocrinol, 220, T1-T23.
3. 3. Tangvarasittichai S. (2015): Oxidative stress, insulin resistance, dyslipidemia and type 2 diabetes mellitus. World J Diabetes, 6 (3), 456-480.
4. 4. Manach C, Scalbert A, Morand C, et al. (2004): Polyphenols: Food sources and bioavailability. Am J Clin Nutr, 79, 727-747.
5. 5. Omar SH. (2010): Oleuropein in Olive and its Pharmacological Effects. Sci Pharm, 78, 133-154.
6. 6. Servili M, Selvaggini R, Esposto S, et al. (2004): Health and sensory properties of virgin olive oil hydrophilic phenols: agronomic and technological aspects of production that affect their occurrence in the oil. J Chromatogr A, 1054, 113-127.
7. 7. Kanter M, Uysal H, Karaca T, et al. (2006): Depression of glucose levels and partial restoration of pancreatic β-cell damage by melatonin in streptozotocin-induced diabetic rats. Arch Toxicol, 80, 362-369.
8. 8. Buchholz T, Melzig MF. (2016): Medicinal Plants Traditionally Used for Treatment of Obesity and Diabetes Mellitus – Screening for Pancreatic Lipase and α-Amylase Inhibition. Phytother Res, 30, 260-266.

9. 9. Satija A, BhupathirajuSN, Rimm EB, et al. (2016): Plant-Based Dietary Patterns and Incidence of Type 2 Diabetes in US Men and Women: Results from Three Prospective Cohort Studies. PLoS Med, 13 (6), 1-18.
10. 10. Al-Azzawie HF, Alhamdani MS. (2006): Hypoglycemic and antioxidant effect of oleuropein in alloxan-diabetic rabbits. Life Sci, 78, 1371-1377.
11. 11. Jemai H, El Feki A, Sayadi S. (2009): Antidiabetic and antioxidant effects of hydroxytyrosol and oleuropein from olive leaves in alloxan-diabetic rats. J Agr Food Chem, 57 (19), 8798-8804.
12. 12. Wainstein J, Ganz T, Boaz M, et al. (2012): Olive leaf extract as a hypoglycemic agent in both human diabetic subjects and in rats. J Med Food, 15, (7) 1-6.
13. 13. Komaki E, Yamaguchi S, Maru I, et al. (2003): Identification of anti-α-Amylase components from olive leaf extracts. Food Sci Technol Res, 9 (1), 35-39.
14. 14. Loizzo MR, Lecce GD, Boselli E, et al. (2011): Inhibitory activty of phenolic compounds from extra virgin olive oils on the enzymes involved in diabetes obesity and hypertension. J Food Biochem, 35, 381-399.
15. 15. Cardinali A, Cicco N, Linsalata V, et al. (2010): Biological activity of high molecular weight phenolics from olive mill wastewater. J Agr Food Chem, 58, 8585-8590.
16. 16. Cheng AYY, Fantus IG. (2005): Oral antihyperglycemic therapy for type 2 diabetes mellitus. Can Med Assoc J, 172 (2), 213-226.
17. 17. Shao J, Iwashita N, Ikeda F, et al. (2006): Beneficial effects of candesartan an angiotensin II type 1 receptor blocker on beta-cell function and morphology in db/db mice. Biochem Bioph Res Co, 344 (4), 1224-1233.
18. 18. Koyuturk M, Ozsoy-Sacan O, Bolkent S, et al. (2005): Effect of glurenorm on immunohistochemical changes in pancreatic beta cells of rats in experimental diabetes. Indian J Exp Biol, 43 (3), 268-271.
19. 19. Kawasaki F, Matsuda M, Kanda Y, et al. (2005): Structural and functional analysis of pancreatic islets preserved by pioglitazone in db/db mice. Am J Physiol-Endoc M, 288 (3), 510-518.
20. 20. Mahmoud MF, El Ashry FZZ, El Maraghy NN, et al. (2017): Studies on the antidiabetic activities of Momordica charantia fruit juice in streptozotocin-induced diabetic rats. Pharm Biol, 55 (1), 758-765.
21. 21. Ahmed ABA, Rao AS, Rao MV. (2010): In vitro callus and in vivo leaf extract of Gymnema sylvestre stimulate β-cells regeneration and anti-diabetic activity in Wistar rats. Phytomedicine, 17, 1033-1039.
22. 22. Girija K, Lakshman K, Chandrika U, et al. (2011): Anti-diabetic and anti-cholesterolemic activity of methanol extracts of three species of Amaranthus. Asian Pac J Trop Biomed, 1 (2), 133-138.
23. 23. Alashkham FA, Osman MT, Adnan A, et al. (2013): Histopathological and Biochemical Effects of Allium Sativum Oil Administration on Type 1 Diabetic rats. Res J Pharm Biol Chem Sci, 4 (1), 1045-1053.