Streptozotosin ile diyabetes mellitus oluşturulan sıçanlarda Allium tuncelianum’un hiperglisemi ve böbrek ve karaciğer dokularında oksidatif stres üzerine etkileri

Abstract

Bu çalışmada streptozotosin ile diyabetes mellitus oluşturulan sıçanlarda Allium tuncelianum ekstraktının hiperglisemi ve böbrek ve karaciğer dokularında oksidatif stres üzerine etkilerini araştırmak amaçlandı. Sıçanlar her bir grupta 10 hayvan olacak şekilde rastgele 4 gruba ayrıldı: Kontrol grubuna (C) periton içi (i.p.) fizyolojik tuzlu su, diyabetli kontrol (DC) grubuna i.p. tek doz 50 mg/kg streptozotosin (STZ), diyabet + insülin (D+I) grubuna i.p. tek doz 50 mg/kg STZ ve 28 gün süre ile deri altı (s.c.) 2 IU insülin (Levemir Flexpen), diyabet + Allium tuncelianum ekstrakt (D+AT) grubuna i.p. tek doz 50 mg/kg STZ ve 28 gün süre ile ağızdan 250 mg/kg Allium tuncelianum ekstraktı ugulandı. Serum glikozile hemoglobin (HbA1c), insülin düzeyleri ve böbrek ve karaciğer tiyobarbitürik asit reaktif maddeler (TBARS), süperoksit dismutaz (SOD) ve katalaz (CAT) aktiviteleri ELISA kitleri ile belirlendi. Kontrol grubuna göre diyabetli kontrol grubunda yüksek kan glikoz düzeyleri (p<0.001), böbrek ve karaciğer homojenatlarında TBARS düzeylerinde artma (p<0.001, p<0.01, sırasıyla) ve SOD ve CAT aktivitelerinde azalma (p<0.001) belirlendi. Allium tuncelianum ekstraktı diyabetli sıçanlarda güçlü antioksidan aktivitelere sahipti ve diyabetli sıçanlarda insülin düzeylerini artırarak, glikozile hemoglobin düzeylerini azaltarak ve oksidatif stresi hafifleterek iyileştirici etkiler gösterdi. Bu çalışma Allium tuncelianum ekstraktının diyabetli hastalar için terapötik potansiyele sahip olabileceğini göstermektedir.

Keywords

• Allium tuncelianum,antioksidan,diyabet,oksidatif stres,sıçan

Effects of Allium tuncelianum on hyperglycemia and oxidative stress in the kidney and liver tissues in rats with diabetes mellitus induced by streptozotocin

Abstract

In this study, it was aimed to investigate the effects of Allium tuncelianum extract on hyperglycemia and oxidative stress in the kidney and liver tissues in rats with diabetes mellitus induced by streptozotocin. The rats were randomly divided into 4 groups with 10 animals in each group: Control group (C) was intraperitoneally (i.p.) treated with physiological saline solution, diabetic control (DC) group i.p. with a single dose of 50 mg/kg streptozotocin (STZ), diabetic + insulin (D+I) group i.p. with a single dose of 50 mg/kg STZ and subcutaneously (s.c.) with 2 IU insulin for 28 days (Levemir Flexpen), diabetic + Allium tuncelianum extract (D+AT) group i.p. with a single dose of 50 mg/kg STZ and orally with 250 mg/kg Allium tuncelianum extract for 28 days. The serum glycated hemoglobin (HbA1c), insulin levels and the kidney and liver thiobarbituric acid reactive substances (TBARS), superoxide dismutase (SOD), and catalase (CAT) activities were determined by using ELISA kits. Increased blood glucose levels (p<0.001), increased TBARS levels (p<0.001, p<0.01 respectively), and decreased SOD and CAT activities (p<0.001) in the kidney and liver tissue homogenates were determined in diabetic control group compared to control group. Allium tuncelianum extract had potent antioxidant activities in the diabetic rats, and demonstrated improvement effects by increasing insulin levels, decreasing glycated hemoglobin levels, and attenuating oxidative stress in the diabetic rats. This study suggests that Allium tuncelianum extract may have therapeutic potential for patients with diabetes.

Keywords

• Allium tuncelianum,antioxidants,diabetes,oxidative stress,rat

References

1. Abdultawab HS, Ayuob NN (2013): Can garlic oil ameliorate diabetes-induced oxidative stress in rat liver model? A correlated histological and biochemical study. Food Chem Toxicol, 59, 650-656.
2. Ahmed R (2005): The physiological and biochemical effects of diabetes on the balance between oxidative stress and antioxidant defense system. Med J Islam World Acad Sci, 15, 31-42.
3. Al-Qattan KK, Mansour MH, Thomson M et al. (2016): Garlic decreases liver and kidney receptor for advanced glycation end products expression in experimental diabetes. Pathophysiology, 23, 135-145.
4. Anwar MM, Meki AR (2003): Oxidative stress in streptozotocin-induced diabetic rats: effects of garlic oil and melatonin. Comp Biochem Physiol A Mol Integr Physiol, 135, 539-547.
5. Aslan M, Orhan N, Deliorman Orhan D et al. (2010): Hypoglycemic activity and antioxidant potential of some medicinal plants traditionally used in Turkey for diabetes. J Ethnopharmacol, 128, 384-389.
6. Augusti KT, Sheela CG (1996): Antiperoxide effect of S-allyl cysteine sulfoxide, an insulin secretagogue, in diabetic rats. Experientia, 52, 115-120.
7. Ayodhya S, Kusum S, Anjali S (2010): Hypoglycemic activity of different extracts of various herbal plants. Int J Ayurveda Res Pharm, 1, 212-224.
8. Badole SL, Ghule AE, Wagh NK (2013): Antidiabetic activity of allium sativum. Bioactive food as dietary interventions for diabetes, http://dx.doi.org/10.1016/B978-0-12-397153-1.00015-9.

9. Chawla A, Chawla R, Jaggi S (2016): Microvascular and macrovascular complications in diabetes mellitus: Distinct or continuum? Indian J Endocrinol Metab, 20, 546-551.
10. Gdula-Argasinska J, Pasko P, Sulkowska-Ziaja K et al. (2017): Anti-inflammatory activities of garlic sprouts, a source of α-linolenic acid and 5-hydroxy-l-tryptophan, in RAW 264.7 cells. Acta Biochim Pol, 64, 551-559.
11. Jakus V (2000): The role of free radicals, oxidative stress and antioxidants systems in diabetic vascular disease. Bratisl Lek Listy, 101, 541-551.
12. Kandemir FM, Ozkaraca M, Küçükler S et al. (2017): Preventive effects of hesperidin on diabetic nephropathy induced by streptozotocin via modulating TGF-β1 and oxidative DNA damage. Toxin Rev, Doi: 10.1080/15569543.2017.1364268.
13. Katsarou A, Gudbjörnsdottir S, Rawshani A et al. (2017): Type 1 diabetes mellitus. Nat Rev Dis Primers, 3, 17016.
14. Kaur G, Padiya R, Adela R et al. (2016): Garlic and resveratrol attenuate diabetic complications, loss of β-cells, pancreatic and hepatic oxidative stress in streptozotocin-induced diabetic rats. Front Pharmacol, 7, 360.
15. Lohwasser C, Neureiter D, Popov Y et al. (2009): Role of the receptor for advanced glycation end products in hepatic fibrosis. World J Gastroenterol, 15, 5789-5798.
16. Maritim A, Sanders R, Watkins J (2003): Diabetes, oxidative stress, and antioxidants: a review. J Biochem Mol Toxicol, 17, 24-38.
17. Metiner K, Ozkan O, Ak S (2016): In vitro antibacterial and antifungal activity of ethanolic Allium tuncelianum extract. Global Veterinaria, 16, 26-30.
18. Morihara N, Hayama M, Fuji H (2011): Aged garlic extract scavenges superoxide radicals. Plant Foods Hum Nutr, 66, 17-21.
19. Ozkan O, Gül S, Kart A et al. (2013): In vitro antimutagenicity of Allium tuncelianum ethanol extract against induction of chromosome aberration by mutagenic agent mitomycine C. Kafkas Univ Vet Fak Derg, 19, 259-262.
20. Patel DK, Prasad SK, Kumar R et al. (2012). An overview on antidiabetic medicinal plants having insulin mimetic property. Asian Pac J Trop Biomed, 2, 320-330.
21. Roh SS, Kwon OJ, Yang JH et al. (2016): Allium hookeri root protects oxidative stress-induced inflammatory responses and β-cell damage in pancreas of streptozotocin-induced diabetic rats. BMC Complement Altern Med, 16, 63.
22. Saravanan G, Ponmurugan P (2010): Beneficial effect of S-allylcysteine (SAC) on blood glucose and pancreatic antioxidant system in streptozotocin diabetic rats. Plant Foods Hum Nutr, 65, 374-378.
23. Shiju TM, Rajesh NG, Viswanathan P (2013): Renoprotective effect of aged garlic extract in streptozotocin-induced diabetic rats. Indian J Pharmacol, 45, 18-23.
24. Sivaraman K, Senthilkumar GP, Sankar P et al. (2013). Attenuation of oxidative stress, inflammation and insulin resistance by Allium sativum in fructose-fed male rats. J Clin Diagn Res, 7, 1860-1862.
25. Takım K (2015): Tunceli Dağ Sarımsağı’nın (Allium tuncelianum) in vitro antioksidan kapasitesinin ölçülmesi, ratlarda antioksidan enzim aktiviteleri üzerine etkisi ve antikanser özelliğinin belirlenmesi. T.C. İnönü Üniversitesi Fen Bilimleri Enstitüsü, Doktora Tezi.
26. Tomita T (2017): Apoptosis of pancreatic β-cells in type 1 diabetes. Bosn J Basic Med Sci, 17, 183-193.
27. Thomson M, Al-Qattan KK, Divya JS et al. (2016): Anti-diabetic and anti-oxidant potential of aged extract (AGE) in streptozotocin-induced diabetic rats. BMC Complement Altern Med, 16, 17.
28. Zahid Ashraf M, Hussain ME, Fahim M (2005): Antiatherosclerotic effects of dietary supplementations of garlic and turmeric: Restoration of endothelial function in rats. Life Sci, 77, 837-857.