Yüksek kolesterollü diyetle beslenen ratlarda öğütülmüş Tribulus terrestris, Avena sativa ve beyazginseng tozunun total kolesterol, serbest testosteron düzeyleri ve testis dokusu üzerine etkileri

Abstract

Bu çalışma, öğütülmüş Tribulus terrestris (TT), Avena sativa (AS), beyaz ginseng (WG) ve üçlü kombinasyon (TC) tozlarının, yüksek kolesterollü diyetle beslenen ratlarda serum total kolesterol, serbest testosteron düzeyleri ve testis dokusunda histopatolojik değişiklikler gibi seksüel fonksiyon bozukluğu parametrelerine etkilerini araştırmak amacıyla yapılmıştır. Araştırmanın hayvan materyali 6 gruba ayrılan, 200-210 g ağırlığında, 42 adet erkek Wistar albino rattan oluştu. Grup I normal pelet yemle beslendi, buna karşın kalan gruplar %2 kolesterol içeren pelet yemle beslendi. Group III, IV, V, ve VI ayrıca sırasıyla 0.6 g/kg/gün TT, 0.3 g/kg/gün AS, 0.2 g/kg/gün WG ve 0.55 g/kg/gün TC (%7.5 TT, %3.75 AS, %2.5 WG) aldı. Doksan gün sonra ratlara ötenazi uygulandı ve kan ve testis doku örnekleri alındı. Serum total kolesterol ve serbest testosteron düzeyleri ölçüldü ve Johnsen testis biyopsi skoru (JTBS), testis doku örneklerinin histopatolojik muayenesi ile hesaplandı. Grup II’de yüksek kolesterollü diyet Grup I’e kıyasla önemli düzeyde total kolesterol düzeyinde artışa ve JTBS’de azalmaya neden oldu. Grupların serbest testosteron düzeyleri istatistiksel olarak önemli olmamasına rağmen, WG ve TC total kolesterol artışını önemli düzeyde önledi. TC tek başına TT, AS ve WG’ye kıyasla JTBS’yi önemli düzeyde artırdı. Dolayısıyla erkek cinsel fonksiyon bozukluğunu iyileştirmek için TC’nin özellikle etkili olabileceği kanısına varıldı

Keywords

• Avena sativa
• ginseng
• hiperkolesterolemi
• testosteron ve seksüel fonksiyon
• Tribulus terrestris

The effects of milled Tribulus terrestris, Avena sativa, and white ginseng powder on total cholesterol, free testosterone levels and testicular tissue in rats fed a high-cholesterol diet

Abstract

This study examines the effects of milled Tribulus terrestris (TT), Avena sativa (AS), white ginseng (WG) and triple-combination (TC) powders on sexual dysfunction parameters ‒ such as serum total cholesterol, free testosterone levels and histopathological changes in testicular tissue ‒ in rats fed a high-cholesterol diet. The study’s animal material consisted of 42 male Wistar albino rats weighing 200‒210 g divided into six groups. Group I was fed normal pellet feed, while the remaining groups were fed pellet feed containing 2% cholesterol. Group III, IV, V, and VI also received 0.6 g/kg/day of TT, 0.3 g/kg/day of AS, 0.2 g/kg/day of WG and 0.55 g/kg/day of TC (7.5% TT, 3.75% AS, 2.5% WG), respectively. After 90 days, the rats were sacrificed and blood and testicular tissue samples obtained. Serum total cholesterol and free testosterone levels were measured, and the Johnsen testicular biopsy score (JTBS) was calculated by a histopathological examination of testicular tissue samples. The high-cholesterol diet in Group II significantly caused increase in total cholesterol level and decrease in JTBS as compared to Group I. Although the groups’ free testosterone levels were not statistically significant, WG and TC significantly prevented total cholesterol increase. TC significantly increased the JTBS compared to TT, AS and WG alone. Thus, it was concluded that TC might be particularly efficient for improving male sexual dysfunction

Keywords

• Avena sativa
• ginseng
• hypercholesterolemia
• testosterone and sexual function
• Tribulus terrestris

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