Immunohistochemical investigation of the damage to and repair of myelin, and astrocyte activity in small ruminants resulting from with natural meningoencephalitic listeriosis

Abstract

Meningoencephalitic listeriosis is a significant bacterial disease in various species. Common characteristics include microabscesses in the brainstem, midbrain and cerebellum. Many aspects of damage to the central nervous system caused by the disease remain obscure. We investigated central nervous system damage by assessing the presence of biomarkers such as galactocerebroside (GAL-C), glial fibrilary acidic protein (GFAP) and myelin basic protein (MBP) in brain tissues of sheep and goats of various ages, which naturally infected with listeriosis. We examined sections of pons, medulla oblongata, rostral colliculus, cerebellum, thalamus and cornu ammonis and found increased MBP (P<0.01), GAL-C (P<0.01) and GFAP (P<0.01) expression. This study showed that myelin damage in meningoencephalitic listeriosis was an important neuropathological finding. The most prominent finding of this study is the beginning of the regeneration as soon as myelin damage occurs

Keywords

• Astrocytes
• GAL-C
• GFAP
• Listeria monocytogenes
• MBP

Küçük ruminantlardaki doğal meningoensefalitik listerioziste miyelin hasar ve onarımı ile astrositaktivitesinin immunohistokimyasal olarak incelenmesi

Abstract

Meningoencephalitic listeriosis çeşitli türlerde önemli bir bakteriyel hastalıktır. Hastalığın karakteristik özelliği beyin sapı, orta beyin ve beyincikte görülen mikroapselerdir. Hastalık sebebiyle merkezi sinir sisteminde meydana gelen hasarlar birçok açıdan hala belirsizliğini korumaktadır. Bu çalışmada, listeriozis ile doğal enfekte değişik yaşlardaki küçük ruminantların merkezi sinir sistemlerinde görülen hasarın varlığı, galaktoserebrosid (GAL-C), glial fibrilary asidik protein (GFAP) ve miyelin temel protein (MBP) gibi biyolojik belirteçlerle araştırıldı. Pons, medulla oblongata, colliculus rostralis, cerebellum, thalamus ve cornu ammonis gibi bölümler incelendi ve beyin hasarını gösteren MBP (P<0.01) ve GFAP (P<0.01) ile birlikte GAL-C (P<0.01) ekspresyonlarının da arttığı görüldü. Bu çalışma, meningoensefalitik listeriyozisdeki miyelin hasarının önemli bir nöropatolojik bulgu olduğunu gösterdi. Araştırmanın en belirgin bulgusu, miyelin hasarı meydana gelirken rejenerasyonun da başladığının gösterilmesidir

Keywords

• Astrosit
• GAL-C
• GFAP
• Listeria monocytogenes
• MBP

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