The effect of fucoidan on changes of some biochemical parameters in nephrotoxicity induced by gentamicin in rats

Abstract

Fucoidan is a polysaccharide with high viscosity and mucilage, which contains significant proportion of sulphate ester group and L-fucose. It is present in intercellular spaces of brown algae. This study aimed to investigate the effect of fucoidan on some biochemical parameters and kidney tissues in nephrotoxicity induced by GM in rats. The rats used in the study were randomly divided into 4 groups. Each group had 7 rats as control, fucoidan, GM and GM+fucoidan. Blood samples were taken after 24 hours from the end of experiment which lasted eight days. Creatinine, BUN, uric acid, glucose, triglycerides, total cholesterol, VLDL, HDL, total bilirubin levels and ALT, AST, ALP, LDH, CK, amylase activities were assayed by an autoanalyser. The kidney tissues were examined histopathologically. In GM+fucoidan group, creatinine (p<0.001), BUN (p<0.001), uric acid (p<0.05), triglycerides (p<0.05), VLDL (p<0.05), AST (p<0.001), ALP (p<0.05), LDH (p<0.001), CK (p<0.01) levels statistically decreased, however HDL (p<0.05) level increased when compared to GM group. As conclusion, it can be said that administering fucoidan during GM toxicity decreases the nephrotoxicity damage. Considering biochemical parameters and histopathological findings, fucoidan can be suggested as a protective agent for kidneys in nephrotoxicity induced rats

Keywords

• Biochemical parameters
• fucoidan
• gentamicin
• nephrotoxicity

Ratlarda gentamisin ile oluşturulan nefrotoksisitede bazı biyokimyasal parametreler üzerinefucoidanın etkisi

Abstract

Fucoidan, tüm kahverengi alglerin hücreler arası boşluklarında bulunan yüksek vizkoziteye sahip müsilajımsı, önemli oranda sülfat ester grupları ve L-fukoz içeren bir polisakkarittir. Bu çalışmada ratlarda gentamisin (GM) ile oluşturulan nefrotoksisitede fucoidan kullanılmasının bazı biyokimyasal parametreler ile böbrek dokusu üzerine etkisinin araştırılması hedeflenmiştir. Çalışmada kullanılan ratlar rastgele seçilerek her biri 7 rattan oluşan 4 gruba ayrıldı: Kontrol grubu, fucoidan grubu, GM grubu, GM+fucoidan grubu. Sekiz günlük deneme süresinden 24 saat sonra kan örnekleri alındı. Kreatinin, BUN, ürik asit, glukoz, trigliserit, total kolesterol, VLDL, HDL, total bilirubin düzeyleri ile ALT, AST, ALP, LDH, CK, amilaz aktiviteleri otoanalizörde analiz edildi. Böbrek dokuları histopatolojik açıdan incelendi. GM grubuna göre fucoidan+GM grubunda kreatinin (p<0.001), BUN (p<0.001), ürik asit (p<0.05), trigliserit (p<0.05), VLDL (p<0.05), AST (p<0.001), ALP (p<0.05), LDH (p<0.001), CK (p<0.01) düzeylerinin istatistiki olarak önemli ölçüde azaldığı, HDL (p<0.05) düzeyinin ise yükseldiği tespit edildi. Sonuç olarak; GM’le oluşturulan nefrotoksisitede incelenen biyokimyasal parametrelere ve böbrek dokusundaki histopatolojik değişimlere bakarak GM’le beraber fucoidan verilmesinin GM'nin oluşturduğu nefrotoksik hasarı zayıflattığı, fucoidanın böbreği koruyucu özelliğinin bulunduğu söylenebilir

Keywords

• Biyokimyasal parametreler
• fucoidan
• gentamisin
• nefrotoksisite

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