In ovo enjekte edilen bisfenol A’nın bir günlük civciv testisleri üzerine etkisi

Abstract

Çevresel östrojenlerden biri olan bisfenol A (BPA) nın memelilerde ve kuşlarda erkek genital sistemi üzerine olumsuz etkileri bulunmaktadır. Tavuk ve bıldırcınlarda in ovo olarak uygulanan yüksek dozda BPA nın sol gonadın ovotestise dönüşmesine sebep olduğu bildirilmiştir. Ancak, in ovo uygulanan BPA nın testiste morfometrik etkileri üzerine bir çalışma bulunmamaktadır. Bu çalışmanın amacı in ovo uygulanan düşük ve yüksek dozdaki BPA nın çıkım yapan civcivlerin testislerinde morfometrik etkilerini ortaya koymaktır. Çalışmada ayrıca karşılaştırma amacıyla iyi bilinen sentetik östrogen dietilstilbestrol (DES) de kullanılmıştır. Enjeksiyonlar (enjeksiyon hacmi 100 µl/yumurta) tavuk yumurtalarına gonadların farklılaşmaya başlamasından önce, kuluçkanın dördüncü gününde uygulanmıştır. Uygulanan dozlar 67 (düşük doz BPA) ve 134 (yüksek doz BPA) µg BPA/gr yumurta, 0.02 (düşük doz DES) ve 0.2 (yüksek doz DES) µg DES/gr yumurtadır. Sertoli ve germ hücresi sayıları stereolojik yöntemlerle belirlenmiştir. Çalışmamızın sonuçları düşük dozda uygulanan BPA nın olumsuz etki oluşturmadığını, tam tersine memelilerdekine benzer bir şekilde kanatlılarda germ hücresi proliferasyonunu arttırdığını göstermiştir. Ancak doz arttığında seminifer tubullerde olumsuz etkilerin açığa çıktığı belirlenmiştir

Keywords

• Bisfenol A
• BPA
• civciv
• dietilstilbestrol (DES)
• testis

Effects of in ovo injected bisphenol A on the testis of one day old chickens

Abstract

Bisphenol-A (BPA), an environmental estrogen, has adverse effects on the male reproductive tract in mammals and birds. In ovo administration of BPA at high doses have been reported to cause the left gonad to form an ovotestis in fowl and quail. However, there are no studies on morphometric effects of in ovo administration of BPA on testis. Therefore, the aim of present study was to investigate the morphometric effects of in ovo administration of low and high doses of BPA on hatched testis. For comparative purposes, well characterized synthetic estrogen diethylstilbestrol (DES) was also examined. Chicken eggs were treated (injection volume 100 µl/egg) on day 4 of incubation before the gonads start to differentiate. Doses were 67 (low BPA group) and 134 (high BPA group) µg BPA/g egg, and 0.02 (low DES group) and 0.2 (high DES group) µg DES/g egg. Sertoli cell and germ cell numbers were determined by stereological methods. Our results indicated that low doses of BPA had no negative effects, on the contrary it was at least increased germ cells proliferation in avian similar to mammals counterpart. However, as doses were titrated upwards, negative effects emerged on seminiferous tubules of testis

Keywords

• Bisphenol A
• BPA
• chicken
• diethylstilbestrol (DES)
• testis

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