Identification of pituitary-specific transcription factor-1 (PIT-1) and leptin gene (LEP) polymorphism of Holstein cattle reared in Turkey

Abstract

Purpose of this study was to examine leptin (LEP) and pituitary-specific transcription factor-1 (Pit-I) gene polymorphisms in Holstein cattle (n=352) in Turkey. In order to determine the Pit-I-HinfI and LEP-Sau3AI polymorphisms, polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) were performed. A 600 bp length fragment of Pit-I and a 422 bp length fragment of LEP were amplified. In this study, two types of alleles, A and B, for both of the Pit-I and LEP genes were observed. The highest frequencies of the alleles were estimated, and were the B allele (0.68) for the Pit-I gene and the A allele (0.87) for the LEP gene. According to the results of the chi-square test, a significant deviation from the Hardy-Weinberg equilibrium was determined for both of these genes in the investigated breed (in the same order; X2:40.75, p<0.001and X2:8.4, p<0.01)

Keywords

• Holstein
• leptin
• pit-I
• polymorphism
• RFLP

Türkiye’de yetiştirilen Holştayn sığırlarda hipofiz bezine özgü transkripsiyon faktörü- 1 (PIT-1) veleptin (LEP) gen polimorfizmlerinin belirlenmesi

Abstract

Bu çalışmada Türkiye’de Kayseri civarında yetiştirilen Holştayn ırkı sığırlarda (n=352) leptin (LEP) ve hipofiz bezine özgü transkripsiyon faktörü -1 (Pit-I) genlerinin polimorfizmlerinin belirlenmesi amaçlanmıştır. Pit-I-HinfI ve LEP-Sau3AI polimorfizmlerinin belirlenmesi amacı ile polimeraz zincir reaksiyonu (PZR) ve restriksiyon parça uzunluk polimorfizm (RFLP) analizi yapılmıştır. Pit-1 geni için 600 bç’lik ve LEP geni için 422 bç’lik uzunluk amplifiye edilmiştir. Çalışmada, Pit-1 ve LEP geni allellerinin her ikisinde de A ve B allelleri olmak üzere iki iki tip allel gözlendi. En yüksek frekans Pit-1 geni için A allelinde (0.87), LEP geni için ise B allelinde (0.68) olarak belirlendi. Ki-kare test sonuçlarına gore, incelenen ırkta, her iki gende de Hardy-Weinberg dengesinden anlamlı bir sapma gözlendi (aynı sıra ile; X2:40.75, p<0.001ve X2:8.4, p<0.01)

Keywords

• Holştayn
• leptin
• pit-1
• polimorfizm
• RFLP

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