Effects of Aroclor 1254 and vitamin E on arginase activity in adult, pregnant rats and their offsprings

Abstract

This study examined the effect of Aroclor 1254 on arginase activity and protective role of vitamin E among adult rats, pregnant rats and their offsprings. Totally 90 Sprague-Dawley rats were divided into three main groups as 30 pregnant, 30 adult and 30 offsprings (pups of the pregnant rats) based on the treatments administered during the pregnancy period (20 days) with subcutaneous daily injections of Aroclor 1254 (2 mg/kg/day) alone or associated with vitamin E (50 mg/kg/day) or normal saline in controls. Female rats and their offsprings (10 offsprings per groups) were analyzed in terms of the arginase activity in liver. A statistically significant increase was determined in liver arginase activity of adult rats exposed to Aroclor 1254 (p<0.05). Vitamin E administered simultaneously with Aroclor enabled liver arginase activity of adult rats to approach statistically normal values. A statistically insignificant increase was found in the arginase activity among pregnant rats and offsprings. While vitamin E administered simultaneously with Aroclor was ineffective in pregnant rats, it caused a statistically significant increase in offsprings (p<0.05). We recommend that addition of vitamin E can prevent the increase in the liver arginase activity caused by Aroclor 1254

Keywords

• Arginase
• Aroclor 1254
• offspring
• pregnant
• vitamin E

Aroclor 1254 ve vitamin E’nin yetişkin, gebe rat ve yavrularında arginaz aktivitesi üzerine etkileri

Abstract

Bu çalışmada yetişkin, gebe rat ve yavrularında arginaz aktivitesi üzerine Aroclor 1254’ün etkisi ve vitamin E’nin koruyucu rolü araştırıldı. Toplam 90 Sprague-Dawley rat 30 yetişkin, 30 gebe ve 30 yavru olmak üzere üç ana gruba ayrıldı. Gebelik süresince (20 gün) subkutan olarak Aroclor 1254 (2 mg/kg/gün) tek başına veya vitamin E (50 mg/kg/gün) ile birlikte ve kontrol grubuna serum fizyolojik uygulandı. Dişi ratlar ve onların yavrularının (her gruptan 10 yavru) karaciğerinde arginaz aktivitesi analiz edildi. Aroclor 1254’e maruz kalan yetişkin ratların karaciğer arginaz aktivitesinde anlamlı bir artış (p<0.05) belirlendi. Aroclor 1254 ile birlikte vitamin E uygulanan yetişkin ratların arginaz aktivitesi istatistiksel olarak normal değerlere yaklaştı. Gebe ratlar ve yavrularının arginaz aktivitesinde istatistiksel olarak anlamsız bir artış tespit edildi. Aroclor 1254 ile birlikte vitamin E uygulanması gebe ratlarda etkili olmasa da; yavru ratlarda anlamlı bir artışa (p<0.05) sebep olmuştur. Vitamin E ilavesinin, Aroclor 1254’ün sebep olduğu karaciğer arginaz aktivitesindeki artışı önleyebildiğini önermekteyiz

Keywords

• Arginaz
• Aroclor 1254
• gebe
• vitamin E
• yavru

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