Immunohistochemical examination on the effects of malathion and Onosma nigricaule (Boraginaceae) on the catalase (CAT) and superoxide dismutase-2 (Mn-SOD) in renal tissues of mice

Abstract

The purpose of this study was to determine the effects of the plant extract, which is obtained from Onosma nigricaule due to the oxidation parameters caused in mice by malathion that is used as an insecticide in agriculture, on catalase (CAT) and superoxide dismutase-2 (Mn-SOD) in kidney tissues by immunohistochemical technique. A total of 48 male mice (Mus musculus) were used in our study. Mice were divided 6 groups (control group, maize oil group, normal saline group, Onosma nigricaule group, malathion group, Onosma nigrcaule+malathion group). Hematoxylin-eosin and triple staining methods were used for histological and pathological examinations. The localization of CAT and Mn-SOD in the renal tissue was determined using the method of streptavidinbiotin-peroxidase. CAT immunoreactivity was determined with a weak intensity in epithelium of renal tubulus proximalis of mice in the malathion group, with a moderate intensity in Onosma nigricaule+malathion group and with a higher intensity in tubulus proximalis of other groups. A cytoplasmic Mn-SOD immunoreactivity was determined with weak intensity in renal medulla of mice in malathion group, with moderate intensity in renal medulla of mice in Onosma nigricaule plant extract+malathion group, maize oil group, and normal saline group and with highly intensity in control and Onosma nigricaule groups. It was concluded that Onosma nigricaule might play a protective role as an antioxidant against the oxidant features of malathion

Keywords

• Catalase
• kidney
• malathion
• mice
• Onosma nigricaule
• superoxide dismutase

Malathion ve Onosma nigricaule (Boraginaceae)’nin fare böbrek dokusunda katalaz (CAT) vesüperoksit dismutaz-2 (Mn-SOD) salınımı üzerine etkileri

Abstract

Bu çalışmada, tarımda insektisit olarak kullanılan ve farelerde oksidasyon parametrelerine neden olan malathiona karşı Onosma nigricaule’den elde edilen bitki ekstraktlarının immunohistokimyasal yöntemle böbrek dokusunda katalaz (CAT) ve süperoksit dismutaz-2 (Mn-SOD) salınımı üzerine etkilerinin saptanması amaçlanmıştır. Çalışmamızda 48 adet erkek (Mus musculus) fare kullanıldı. Fareler 6 gruba (kontrol grubu, mısır yağı grubu, serum fizyolojik grup, Onosma nigricaule grubu, malathion grubu, Onosma nigricaule+malathion grubu) ayrıldı. Histolojik ve patolojik değerlendirmeler için hematoksilen-eozin and triple boyama yöntemleri kullanıldı. Böbrek dokusunda CAT ve Mn-SOD lokalizasyonu için ise streptavidin-biotin- peroxidase yöntemi kullanıldı. CAT immunoreaktivitesinin fare böbrek dokusunun tubulus proksimalisinde olduğu görüldü. CAT immunoreaktivitesinin malathion grubunda zayıf, malathion+Onosma nigricaule grubunda orta yoğunlukta, diğer gruplarda ise yüksek yoğunlukta olduğu tespit edildi. Mn-SOD immunoreaktivitesinin böbrek medullasında olduğu görüldü. Malathion grubunda zayıf, mısır yağı grubu, serum fizyolojik ve Onosma nigricaule+malathion gruplarında orta yoğunlukta, kontrol ve Onosma nigricaule gruplarında ise yüksek yoğunlukta olduğu tespit edildi. Onosma nigricaule’nin malathionun oksidadif etkilerine karşı antioksidan gibi koruyucu bir rol oynadığı sonucuna varılabilir

Keywords

• Böbrek
• fare
• katalaz
• malathion
• Onosma nigricaule
• süperoksit dismutaz

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