Investigation of Neospora caninum tissue cysts in cattle

Abstract

In this study, it was aimed to detect N. caninum seroprevalence in 200 cattle randomly selected and to detect tissue cysts of N. caninum in tissue samples of brain and skeletal muscles of the seropositive cattle. Seropositivity of N. caninum was detected in 5.5% of cattle (11/200). N. caninum tissue cysts were not observed in tissue samples of cattle examined with percoll gradient centrifugation. However, N. caninum DNA was amplified in brain tissues of two seropositive bulls (18.1%).Özet: Bu çalışmada rastgele seçilen 200 sığırda N. caninum seropozitivitesinin belirlenmesi, seropozitif sığırların beyin ve
iskelet kası doku örneklerinde N. caninum doku kistlerinin percoll gradient santrifüj ve PCR yöntemleri ile araştırılması amaçlanmıştır.
Çalışma sonucunda sığırlarda %5,5 oranında N. caninum seropozitivitesi belirlenmiştir (11/200). Percoll gradient santrifüj yöntemi ile
incelenen seropozitif sığırlara ait doku örneklerinde parazite ait doku kistleri tespit edilmemiştir. Ancak iki seropozitif boğanın beyin
dokusunda N. caninum’a ait DNA amplifiye edilmiştir (%18.1). 

Keywords

• Cattle
• Neospora caninum
• serology
• tissue cyst

Sığırlarda Neospora caninum doku kistlerinin araştırılması

Abstract

Bu çalışmada rastgele seçilen 200 sığırda N. caninum seropozitivitesinin belirlenmesi, seropozitif sığırların beyin ve iskelet kası doku örneklerinde N. caninum doku kistlerinin percoll gradient santrifüj ve PCR yöntemleri ile araştırılması amaçlanmıştır. Çalışma sonucunda sığırlarda %5,5 oranında N. caninum seropozitivitesi belirlenmiştir (11/200). Percoll gradient santrifüj yöntemi ile incelenen seropozitif sığırlara ait doku örneklerinde parazite ait doku kistleri tespit edilmemiştir. Ancak iki seropozitif boğanın beyin dokusunda N. caninum’a ait DNA amplifiye edilmiştir (%18.1)

Keywords

• Doku kisti
• Neospora caninum
• seroloji
• sığır

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