Genotyping of Echinococcus granulosus obtained from cattle slaughtered in Kırıkkale Province

Abstract

Strain differences are responsible from some important differences such as host specifity, growth rate, antigenic properties, sensitivity to chemotherapeutic agents, transmission dynamics and epidemiological variations in Echinococcus granulosus. In this study, it was aimed to detection strain identification of E. granulosus obtained from liver and lungs of cattle slaughtered in Kırıkkale region. For this aim, mitochondrial cytochrome c oxidase subunit 1 (COI) and 12S rRNA genes of E. granulosus isolates amplified by specific primers and then COI gene sequence was made. In the present study, the isolates were identified as G1-G3 complex according to COI sequencing results

Keywords

• Cattle
• Echinococcus granulosus
• genotyping
• hydatid cyst
• Kırıkkale

Kırıkkale’de kesilen sığırlarda Echinococcus granulosus’un moleküler olarak genotiplendirilmesi

Abstract

Echinococcus granulosus türü içindeki suş farklılıkları etkenin konak spesifitesi, gelişim hızı, patojenitesi, antijenik özellikleri, kemoterapotiklere duyarlılığı, bulaşma dinamikleri ve epidemiyolojide farklılıklar şekillendirmektedir. Bu çalışmada; Kırıkkale Yöresi’nde kesilen sığırların karaciğer ve akciğerlerinden elde edilen E. granulosus izolatlarının suş identifikasyonu yapılması amaçlanmıştır. Bunun için parazitin 12S rRNA ve mitokondriyal cytochrome c oxidase subunit 1 (COI) geni spesifik primerlerle çoğaltılmıştır ve COI geni sekanslatılmıştır. COI sekans sonucuna göre, elde edilen izolatların G1-G3 kompleksinde olduğu belirlenmiştir

Keywords

• Echinococcus granulosus
• genotiplendirme
• hidatid kist
• Kırıkkale
• sığır

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