The effects of Kisspeptin on LH profile and reproductive parameters in Pirlak sheep

Abstract

In this study, it was investigated, the effects of application of Kisspeptin-10 (Kp-10) on the luteinizing hormone (LH) profiles and reproductive parameters. Total 60 Pirlak sheep were allocated into 3 equal (n=20) groups in breeding seasons. Estrous cycles were synchronized with double PGF2α method. Following a second cloprostenol injection, the Group 1 was treated with human kisspeptin-10 (1µg/kg) once at 40th h but the Group 2 was treated with Kp-10 (1µg/kg) twice at 40th and 50th h. The Group 3 was not treated with Kp-10 but treated with serum physiologic. All group of ewes were mated between 42nd and 43rd hours by hand mating system. Onset of LH surge and peak time of LH occured similar time in all groups. Group 2 plasma levels of LH at 50.5 h following last cloprostenol was found higher than Group 1 and Group 3 (p=0.005). Preovulatory LH peak concentration of Group 2 was found higher than Group 1 and Group 3 (p=0.030). Duration of LH surge was 18,63 ± 7,21, 18,03 ± 5,00 and 12,55 ± 6,93 h respectively in Group 1, 2 and 3 (p=0.009). Group 1and Group 2 of the area under the curve for LH (LH AUC) at 55 h were higher than Group 3 (p=0.042). Progesterone (P4), estradiol (E2) profiles and oestrus, conception, fecundity and prolificacy rates were found to be similar (p> 0.05) between the all groups. As a result, it was found that Kp-10 can be affect LH profiles but had no significant change on reproductive parameters

Keywords

• Kisspeptin 10
• Pirlak sheep
• preovulatory LH
• reproductive parameters

Pırlak koyunlarda Kisspeptinin LH profili ve reprodüktif parametrelere etkileri

Abstract

Bu çalışmada, Kisspeptin-10 (Kp-10) uygulamasının LH profili ve reprodüktif parametrelere olan etkileri araştırıldı. Üreme sezonunda 60 baş pırlak koyunu 3 eşit (n=20) gruba bölündü. Tüm grupların östrus senkronizasyonu çift doz PGF2α yöntemi ile gerçekleştirildi. Grup 1’e, ikinci PGF2 α enjeksiyonunu takip eden 40. saatte bir kez Kp-10 (1 µg/kg, i.v), Grup 2’ye 40 ve 50. saat olmak üzere iki kez Kp-10 (1 µg/kg, i.v) uygulaması gerçekleştirildi. Grup 3’e ise Kp-10 uygulaması yapılmadı, serum fizyolojik uygulandı. Tüm gruplardaki koyunlar 42-43. saatler içerisinde elde sıfat yöntemiyle çiftleştirildi. Grup 2’nin son PGF2 α enjeksiyonunu takip eden 50,5. saat plazma LH düzeyi Grup 1 ve Grup 3’e göre daha yüksek bulundu (p=0.005). Preovulatör LH dalga başlangıcı ve LH pik zamanı tüm gruplarda benzer saatte gerçekleşti. Grup 2’nin preovulatör LH pik düzeyi grup 1 ve grup 3’e göre daha yüksek tespit edildi (p=0.030). Grup 1, 2 ve 3’te LH dalga süresi sırasıyla 18,63±7,21, 18,03±5,00 and 12,55±6,93 saat (p=0.009) olarak belirlendi. Grup 1 ve Grup 2’nin 55. saat toplam salınan LH düzeyi (LH AUC) Grup 3’e göre daha yüksek bulundu (p= 0.042). Gruplar arasında progesteron (P4) ve östradiol (E2)profilleri ile östrus, gebe kalma, fekondasyon ve prolifikasyon oranları benzer bulundu. Sonuç olarak, Kp-10’nun LH profilini önemli derecede etkileyebildiği, ancak, reprodüktif parametreler üzerinde önemli bir değişikliğe yol açmadığı belirlendi

Keywords

• Kisspeptin 10
• Pırlak koyunu
• preovulatör LH
• reprodüktif parametre

References

1. Ben Said S, Lomet D, Chesneau D, et al. (2007): Differential estradiol requirement for the induction of estrus behavior and the luteinizing hormone surge in two breeds of sheep. Biol Reprod, 76, 673-680.
2. Caraty A, Franceschini I (2008): Basic aspects of the control of GnRH and LH secretions by kisspeptin: Potential applications for better control of fertility in females. Reprod Domest Anim, 43, 172-178.
3. Caraty A, Smith JT, Lomet D, et al. (2007): Kisspeptin Reprod Domest Anim, 43, 308-313. synchronizes preovulatory surges in cyclical ewes and with causes ovulation in seasonally acyclic ewes. Endocrinology, reproductive performance of awassi ewes synchronized 148, 5258-5267. exogenous GnRH at the time of artificial insemination on
4. Christian CA, Moenter SM (2010): The neurobiology of preovulatory releasing hormone surges. Endocr Rev, 31, 544-77.
5. Clarke IJ (2011): Control of GnRH secretion: One step back. Front Neuroendocrinol, 32, 367-375.
6. Contreras-Solis I, Vasquez B, Diaz T, et al. (2009): Ovarian and endocrine responses in tropical sheep treated with reduced doses of cloprostenol. Anim Reprod Sci, 114, 384-92.
7. De Rensis F, Valentini R, Gorrieri F, et al. (2008): Inducing ovulation with hCG improves the fertility of dairy cows during the warm season. Theriogenology, 69, 1077- 1082.
8. Fields SD, Perry BL, Perry GA (2009): Effects of GnRH treatment on initiation of pulses of LH, LH release, and subsequent concentrations of progesterone. Domest Anim Endocrinol, 37, 189-195.

9. Gonzalez-Bulnes A, Veiga-Lopez A, Garcia P, et al. (2005): Effects of progestagens and prostaglandin analogues on ovarian function and embryo viability in sheep. Theriogenology, 63, 2523-2534.
10. Goodman R, Inskeep EK (2006): Neuroendocrine control of the ovarian cycle of the sheep. pp. 2389-2447. Knobil and Neill's Physiology of Reproduction. Elsevier, USA.
11. Hashizume T, Saito H, Sawada T, et al. (2010): Charac- teristics of stimulation of gonadotropin secretion by kisspeptin-10 in female goats. Anim Reprod Sci, 118, 37-41.
12. Letelier CA, Contreras-Solis I, García-Fernández RA, et al. (2011): Effects of oestrus induction with progestagens or prostaglandin analogues on ovarian and pituitary function in sheep. Anim Reprod Sci, 126, 61-69.
13. Olivera-muzante J, Gil J, Fierro S, et al. (2011): Alternatives to improve a prostaglandin-based protocol for timed artificial insemination in sheep. Theriogenology, 76, 1501-1507.
14. Paksoy Z, Kalkan C (2010): The effects of GnRH and hCG used during and after artificial ınsemination on blood serum progesterone levels and pregnancy rate in cows. Kafkas Univ Vet Fak Derg, 16, 371-375.
15. Perry GA, Perry Bl (2009): GnRH treatment at artificial insemination in beef cattle fails to increase plasma progesterone Theriogenology, 71, 775-779. or pregnancy rates.
16. Roa J, Tena-Sempere M (2007): KiSS-1 system and reproduction: Comparative aspects and roles in the cont- rol of female gonadotropic axis in mammals. Gen Comp Endocrinol, 153, 132-40.
17. Salem IB, Rekik M, Gonzalez-Bulnes A, et al. (2010): Differences in preovulatory follicle dynamics and timing of preovulatory LH surge affect fertility of maiden sheep reared in semi-arid extensive conditions. Anim Reprod Sci, 117, 60-66.
18. Smith JT, Li Q, Yap KS, et al. (2011): Kisspeptin is essential for the full preovulatory LH surge and stimulates GnRH release from the isolated ovine median eminence. Endocrinology, 152, 1001-1012.
19. progestagen-PMSG-PGF2 alpha combination.
20. Yıldız S, Saatci M, Uzun, M, et al. (2003): Effects of ram introduction after the second prostaglandin f2 alpha injection on day 11 on the LH surge characteristics in fat-tailed ewes. Reprod Domest Anim, 38, 54-57.