Effects of hypothyroidism and hyperthyroidism on hematological parameters in rats

Abstract

In this study, it was aimed to interpret the effects of hypothyroidism and hyperthyroidism on hematological parameters in rats. A total of 42 male Wistar rats were divided into 3 equally groups as euthyroid, hypothyroid, hyperthyroid. Hypothyroidism and hyperthyroidism were induced by 6-n-propyl-2-thiouracil (PTU; 0,05%, w/v) and L-thyroxine (L-T4; 0,0012%, w/v) received in drinking water, respectively. Blood samples were withdrawn from 7 rats of each group at the end of 3rd and 6th weeks for determination of the hematological parameters and serum hormone profiles. Among the 3rd week groups only percentage of monocyte was increased (p<0.05) in the hypothyroid group when compared to the euthyroid group. In the 6th week hypothyroid group, red blood cell (RBC) counts, hemoglobin (Hb) concentration, hematocrit (Hct) value and percentage (%) of red cell distribution width (RDW) were lower (p<0.05) than the 6th week euthyroid and hyperthyroid groups. However, plateletcrit (PCT) % was higher (p<0.05) in the 6th week hypothyroid group compared to the 6th week euthyroid group. In the 6th week hyperthyroid group, mean corpuscular hemoglobin concentration (MCHC) and platelet (PLT) counts were lower (p<0.05) than the 6th week hypothyroid group. In the 6th week hyperthyroid group percentage of lymphocyte was decreased (p<0.05) and percentage of granulocyte was increased (p<0.05) when compared to the 6th week euthyroid group. In conclusion, elongation of the treatment duration with PTU and L-T4 could lead to changes on some hematological parameters. However, these values remained within physiological ranges

Keywords

• Propylthiouracil
• rat hematology
• red blood cells
• thyroid status

Ratlarda hipotiroidizm ve hipertiroidizmin hematolojik parametreler üzerine etkileri

Abstract

Bu çalışmada, hipotiroidizm ve hipertiroidizmin ratların hematolojik parametreleri üzerine etkilerini yorumlamak amaçlanmıştır. Toplam 42 adet erkek Wistar rat, ötiroid, hipotiroid ve hipertiroid olarak 3 eşit gruba ayrıldı. Hipotiroidizm ve hipertiroidizm sırasıyla 6-n-propil-2-tiourasil (PTU; % 0,05, w/v) ve L-tiroksin (T4; % 0,0012, w/v) içme sularına katılarak gerçekleştirildi. Hematolojik parametrelerin ve serum hormon profillerinin belirlenmesi için her grupta 7 rattan, 3. ve 6. haftanın sonunda kan örnekleri toplandı. Üçüncü hafta grupları arasında, yalnızca hipotiroid grubunun monosit yüzdesi ötiroid grubuna kıyasla arttı (p<0.05). Altıncı hafta hipotiroid grubunda, alyuvar (RBC) sayısı, hemoglobin (Hb) konsantrasyonu, hematokrit (Hct) yüzdesi ve alyuvar dağılım genişliği (RDW) yüzdesi, 6. hafta ötiroid ve hipertiroid grubuna nazaran düşüktü (p<0.05). Ancak, 6. hafta hipotiroid grubundaki plateletkrit (PCT) yüzdesi, 6. hafta ötiroid grubuna kıyasla yüksekti (p<0.05). Altıncı hafta hipertiroid grubunda, ortalama alyuvar hemoglobin konsantrasyonu (MCHC) ve platelet (PLT) sayısı 6. hafta hipotiroid grubuna nazaran düşüktü (p<0.05). Altıncı hafta hipertiroid grubunda, 6. hafta ötiroid grubuna kıyasla lenfosit yüzdesi düşük (p<0.05) ve granülosit yüzdesi yüksekti (p<0.05). Sonuç olarak, propiltiourasil (PTU) ve L-tiroksin (L-T4) uygulama sürelerinin uzaması bazı hematolojik parametrelerde değişimlere sebep olmaktadır. Buna rağmen, bu değerler fizyolojik sınırlar içerisinde kalmaktadır

Keywords

• Kırmızı kan hücreleri
• propiltiyourasil
• rat hematolojisi
• tiroid bozukluğu

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