Investigation of antimicrobial resistance and extended spectrum beta- lactamase production inEscherichia coli isolates of equine feces origin

Abstract

In this study, it was aimed to investigate the existence of ESBL and determine the resistance against various antibiotics of E. coli strains isolated from feces examples obtained from racing and jumping horses. One hundred E. coli isolates (racing: 37, jumping: 63) were analysed with 16 antibiotics by using the disc diffusion method and ESBL existence by fenotypic confirmatory test. The antibiotic resistance prevalences were compared between racing and jumping groups of horses. The highest resistance was obtained against tetracycline in both groups with 81.1% (30) in racing horses and 20.6% (13) in jumping horses. ESBL production has been determined in only 6 isolates among 100 E. coli isolates and all 6 ESBL positive isolates were isolated from the racing horses. In conclusion, the resistance prevalences to various antibiotics in racing horses were higher than jumping horses and ESBL production in the isolates from jumping horses was determined. The contamination of these agents in ecosystem could cause a potential risk factor for public health

Keywords

• Antibiotic resistance
• disc diffusion
• E. coli
• ESBL

At fekal orijinli Escherichia coli izolatlarında antimikrobiyal direnç vegenişlemiş spektrumlu beta laktamaz üretiminin araştırılması

Abstract

Bu çalışma, düz yarış ve konkur atlarından alınan fekal örneklerden izole edilen Escherichia coli izolatlarının çeşitli antibiyotiklere direnç durumlarını belirlemek ve genişlemiş spektrumlu beta laktamaz (GSBL) varlığını araştırmak amacıyla yapıldı. Çalışmada izole edilen 100 E. coli (düz yarış: 37, konkur: 63) izolatının 16 antibiyotiğe karşı duyarlılıkları disk difüzyon yöntemiyle, GSBL varlığı ise fenotipik doğrulama testi ile belirlendi. Düz yarış ve konkur grupları arasında antibiyotik direnç oranları karşılaştırıldı. En yüksek antibiyotik direnci, düz yarış atlarında %81.1 (30/37) ve konkur atlarında %20.6 (13/63) oranlarında olmak üzere tetrasikline karşı belirlendi. Atlardan izole edilen 100 E. coli izolatının ise 6 (%6) tanesinde GSBL üretimi fenotipik olarak doğrulandı. GSBL pozitif izolatların tümü düz yarış atlarından izole edildi. Sonuç olarak, düz yarış atlarının fekal florasından elde edilen E. coli izolatlarında çeşitli antibiyotiklere direnç daha yüksek oldu ve bu izolatlarda GSBL üretimi belirlendi. Bu etkenlerin ekosisteme bulaşması halk sağlığı açısından potansiyel bir risk oluşturabilir

Keywords

• Antibiyotik direnci
• disk difüzyon
• Escherichia coli
• GSBL

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