Köpeklerde deri ve eklenti tümörlerinin değerlendirilmesinde ince iğne aspirasyon biyopsisi (İİAB)bulgularının histopatolojik bulgularla karşılaştırılması

Abstract

Bu çalışmada, Ankara Üniversitesi Veteriner Fakültesi’ne ve Ankara’da bulunan özel veteriner kliniklerine deri tümörü şüphesiyle getirilen 43 köpeğe ait biyopsi örneği sitopatolojik ve histopatolojik olarak incelendi. Sitopatolojik örnekler sedasyon altında ince iğne aspirasyon biyopsisi (İİAB) tekniği ile alınarak May-Grünwald Giemsa (MGG) ile boyandı. Histopatolojik inceleme amacıyla alınan doku örnekleri %10’luk nötral formalin solüsyonunda tespit edilip, rutin doku takibine alınarak hematoksilen-eozin (H&E) ile boyandı. Tümörlerin alındığı köpeklerden 29’u erkek olup 14’ü ise dişiydi. Tümör görülme oranının 8-15 yaşları arasında arttığı gözlendi. Köpek ırkları incelendiğinde; köpeklerden 14’ü terrier, 14’ü melez ve 15’i diğer ırklardandı. Lokalizasyon olarak tümörler daha çok kafa, perianal bölge ve ön-arka bacaklarda yerleşim göstermekteydi. Sitopatolojik olarak toplam tümörlerden 30 (%69,76)’una malign epitelyal tümör, 5 (%11,62)’ine benign epitelyal tümör, 6 (%13,95)’sına epitelyal tümör şüpheli ve 2 (%4,65)’sine hafif malign epitelyal tümör tanısı konuldu. Histopatolojik olarak yapılan tanılar doğrultusunda ise tespit edilen tümörler; 12 (%27,9) yassı hücreli kanser, 9 (%20,9) hepatoid bez tümörü, 7 (%16,3) bazal hücreli kanser, 7 (%16,3) ter bezi tümörü, 5 (%11,6) sebaseöz bez tümörü, 2 (% 4,7) papillom ve 1 (%2,3) malignant pilomatrikom olarak belirlendi. Çalışma sonucunda sitopatolojik ve histopatolojik olarak yapılan tanıların karşılaştırmasında %86,05 oranında her iki tanının birbirleriyle örtüştükleri tespit edildi. Sonuç olarak, bu çalışma ile sitopatolojik tanı yönteminin köpeklerde deri tümörlerinin erken tanısında kullanılabileceği düşünülmektedir

Keywords

• Deri tümörü
• histopatoloji
• ince iğne aspirasyon biyopsisi
• köpek
• sitopatoloji

A comparative study between fine needle aspiration biopsy (FNAB)findings and histopathology in the evaluation of canine skin and skinadnexal tumors

Abstract

The material of this study was, cytopathologic and histopathologic specimens obtained from 43 of dogs suffering from skin tumors. Samples were submitted by Ankara University, Faculty of Veterinary Medicine or private clinics of veterinary in Ankara. Cytologic specimens were prepared by fine needle aspiration biopsy (FNAB) technique and stained with May-Grünwald Giemsa (MGG). For histopathologic examinations tissue samples were obtained by surgery. Samples were fixed in 10% neutral formalin solution and embedded in paraffin by routine procedures and stained with Hematoxylin-Eosin (H&E). Tumors were obtained from 29 male dogs and 14 female dogs. The age with highest incidence in dogs was between 8 and 15. The breeds of dogs more frequently affected were 14 terrier, 14 mongrel and 15 another different breeds. Localizations of tumors were in the followed regions, head, perianal regions and legs. Cytopathologically, 30 (69,76%) malignant epithelial tumors, 5 (11,62%) benign epithelial tumors, 6 (13,95%) suspect epithelial tumors and 2 (4,65%) few malignant epithelial tumors were diagnosed. Histopathological diagnosis determined 12 (27,9%) squoamous cell carcinoma, 9 (20,9%) hepatoid gland tumours, 7 (16,3%) basal cell carcinoma, 7 (16,3%) sweat gland tumours, 5 (11,6%) sebaceous gland tumours, 2 (4,7%) papilloma and 1 (2,3%) malignant pilomatricoma. The diagnoses were in agreement cytopathologically and histopathologically in 86,05% of cases. The results obtained in the present study suggest that cytopathology is a practical tool for the early diagnosis of canine skin tumours

Keywords

• Cytopathology
• dog
• fine needle aspiration biopsy
• histopathology
• skin tumours

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