Köpek hepatoid bezi tümörlerinin tanısında ince iğne aspirasyon sitolojisi (İİAS): Histopatolojiyle karşılaştırmalı çalışma

Abstract

Bu çalışmada hepatoid bez tümörlerinin sitolojik ve histopatolojik bulguları karşılaştırılarak sitolojik tanının güvenirliği araştırıldı. Bu amaçla hepatoid bez tümörü şüphesi bulunan 32 farklı ırk, yaş ve cins köpekten alınan örnekler kullanıldı. Sitolojik örnekler İnce İğne Aspirasyon Sitolojisi (İİAS) tekniğine göre alındı, havada kurutularak May-Grünwald Giemsa (MGG) ile boyandı. Histopatolojik inceleme amacıyla alınan dokular ise %10’luk nötral formalin solüsyonunda tespit edilerek rutin doku takibine alınarak hematoksilen eozin (HE) ile boyandı. Çalışma sonucunda; 32 örneğin %84.37’sinde sitolojik ve histopatolojik tanının örtüştüğü, buna karşılık 5 olguda hatalı tanıya gidildiği görüldü. Sitolojik olarak yapılan incelemelerde 27 örneğin 18 (%66,7)’ ine adenom, 6 (%22,2)’ine karsinom ve 3 (%11,1)’üne benign-epitelyom tanısı konuldu. Histopatolojik olarak ise 32 olgunun 22 (%68,7)’ü hepatoid gland adenom (HGA), 7 (%21,9)’i hepatoid gland karsinom (HGK) ve 3 (%9,4) hepatoid gland epitelyom (HGE) olarak tespit edildi. Sonuç olarak köpeklerde hepatoid tümörlerde İİAS tekniği ve sitolojik tanının erken tanıda yararlı olabileceği kanısına varıldı

Keywords

• hepatoid bez tümörü, histopatoloji, ince iğne aspirasyon sitolojisi, köpek, malignite

Fine needle aspiration cytology (FNAC) in the diagnosis of canine hepatoid gland tumors- A comparative study with histopathology

Abstract

In this study, cytological and histological findings of canine hepatoid gland tumors were evaluated for accuracy of cytologic diagnosis. Thirty two samples from dogs of different breed, sex and age were used in the study. Cytologic speciments were prepared by Fine Needle Aspiration Cytology (FNAC) and stained with May-Grünwald Giemsa (MGG). On the other hand, histopathologic tissue samples were obtained surgically. Samples were fixed in 10% neutral formalin solution and embedded in paraffin by routine procedures and stained with hematoxylin-eosin (H&E). In 84.37% of the samples taken from the 32 dogs included in the study, matching cytological and histopathological diagnoses were made, while 5 of the cases were misdiagnosed. Based on cytological examination, from 27 of the samples taken, 18 (66,7%) were diagnosed as Suspect adenoma, 6 (22,2%) as Suspect carcinoma and 3 (11,1%) as Suspect epithelioma. Based on histopathological examination, of the total 32 cases, 22 (68,7%) were diagnosed as Hepatoid gland adenoma (HGA), 7 (21,9%) as Hepatoid gland carcinoma (HGC) and 3 (9,4%) as Hepatoid gland epithelioma (HGE). These results suggest that the FNAC technique and cytological examination could be of use in the early diagnosis of canine hepatoid gland tumors

Keywords

• Dog, fine-needle aspiration cytology, hepatoid gland tumor, histopathology, malignancy

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