Türkiye’de abortla ilişkili equid herpesvirus tip 1 izolatlarının gB geninin kısmi sekansı

Abstract

Equid herpesvirus 1 (EHV-1), kısraklarda sporadik veya epidemik tarzda genellikle gebeliğin son trimesterinde görülen abort olgularından sorumlu başlıca etkendir. Bu araştırmada, yeni dizayn edilen bir primer çifti kullanılarak, ülkemizde 2011 yılındaki doğum sezonundaki abort salgınından izole edilen Türkiye (TR) EHV-1 saha suşlarının glikoprotein B (gB) gen bölgesinin kısmi karakterizasyonu araştırılmıştır. Moleküler analiz sonuçları bu araştırmada izole edilen suşların, önceki yıllarda ülkemizde izole edilen EHV-1 suşlarına genetik olarak benzer; EHV-1 Avrupa suşlarıyla ise genetik yakınlığa sahip olduğunu göstermiştir. Ayrıca, bu araştırmada izole edilen suşlar ile GenBank veri tabanında kayıtlı diğer EHV-1 suşlarının aminoasit sekansları arasında farklılıklar tespit edilmiştir. Bu moleküler araştırma, ülkemizde EHV-1 enfeksiyonunun izlenmesi ve gelecekteki salgınlardan izole edilebilecek yeni suşların gB gen bölgesindeki değişikliklerin patogenezi ve hastalığın gelişimi üzerine etkilerinin belirlenmesine ışık tutacaktır

Keywords

• Abort, at, at herpesvirus tip 1 (EHV-1), glikoprotein B geni, Türkiye

Partial sequence of the gB gene of equid herpesvirus type 1 isolates associated with abortion in Turkey

Abstract

Equid herpesvirus 1 (EHV-1) is a major agent of large-scale outbreaks of abortion, and these abortions have been described as sporadic or epidemic cases in mares, generally during the last trimester of pregnancy. In this study, the partial characterization based on the glycoprotein B (gB) gene of Turkish (TR) EHV-1 field strains isolated from an abortion outbreak during the 2011 foaling season in Turkey was investigated using a novel designed primer set for EHV-1. The molecular analysis of TR EHV-1 strains showed that genetically identical TR EHV-1 strains were still circulating in Turkey during different years and, these strains were closely related to the European EHV-1 strains. Furthermore, EHV-1 isolated in the present study and to EHV-1 strains published previously in the GenBank database were shown some differences for the aa sequences. This molecular report would be valuable for monitoring of EHV-1 infection in Turkey and determining the gB gene sequence of newly identified EHV-1 field strains from future outbreaks on the pathogenesis and severity of disease

Keywords

• Abortion, equid herpesvirus type 1 (EHV-1), glycoprotein B gene, horse, Turkey

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