Genç ve yaşlı köpeklerin yağ dokusundan elde edilen mezenkimal kök hücrelerin karakterizasyonu

Abstract

Mezenkimal kök hücrelerin veteriner hekimlik alanında kullanımı birçok hastalığın tedavisi için umut ışığı olarak görülmektedir. Yağ doku kökenli mezenkimal kök hücrelerin eldesinin kolay olması ve üretiminin ek bir maliyet getirmemesi sebebiyle yağ dokudan kök hücre üretimi en çok kullanılan yöntem olarak karşımıza çıkmaktadır. Yağ doku kökenli hücrelerin multilineage potansiyeline sahip olduğu bilinmektedir. Köpek yağ doku kaynaklı mezenkimal kök hücrelerin başarılı bir şekilde transplante edildiği de daha önceki çalışmalarda da bildirilmiştir. Bu çalışmada 3 adet yaşlı (5 yaş üzeri) ve 3 adet genç (6 ay ve altı) köpekten operasyon sırasında alınan yağ doku örneklerinden elde edilen mezenkimal kök hücrelerin karşılaştırılması, adipojenik, kondrojenik ve osteojenik farklılaşma potansiyeli, farklılaşma hızı, üreme hızı ve karakteristik özellikleri yönünden aralarındaki farkların ortaya konulması, lipoaspiratın içerdiği kök hücre potansiyelinin karşılaştırılması amaçlanmıştır

Keywords

• Farklılaşma, köpek, mezenkimal kök hücre, yağ doku

Characterization of mesenchymal stem cells isolated from the adipose tissue of young and old dogs

Abstract

The use of mesenchymal cells in veterinary medicine is considered to be promising for the treatment of several diseases. Due to adipose mesenchymal stem cells being readily available and the expansion of these cells not incurring any additional cost, the culturing of adipose mesenchymal cells has become a commonly applied method. Adipose mesenchymal cells are known to have a multilineage potential of differentiation. Previous research has demonstrated success in the transplantation of canine adipose mesenchymal cells. This study was aimed at the comparative evaluation of mesenchymal stem cells isolated from adipose tissue samples taken at surgery from 3 old dogs (above 5 years of age) and 3 young dogs (1 to 6-months-old or younger), and at the determination of any differences in the adipogenic, chondrogenic, and osteogenic differentiation potential, differentiation rate, proliferation rate, and characteristics of these cells. Furthermore, a comparison was made for the stem cell potential of the lipoaspirates

Keywords

• Adipose tissue, differentiation, dog, mesenchymal stem cell

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