Koyun beta-laktoglobulin geni ekzon 2 bölgesine ait genetik polimorfizmin araştırılması ve bazı süt verim özellikleri ile ilişkisi

Abstract

β-laktoglobulin (β-LGB) geni ruminantlarda en temel peynir altı suyu protenidir; gebelik ve laktasyon süresince meme bezi hücrelerinden salgılanmaktadır; biyolojik fonksiyonu ise henüz tam olarak bilinmemektedir. Dolayısıyla, bu çalışmanın amacı koyun β-LGB geni ekzon 2 bölgesine ait tek nükleotit polimorfizmlerinin (TNP) genotip frekanslarının saptanması ve bu olası TNP’lerin süt verim özellikleri ile ilişkisinin belirlenmesidir. Çalışmada üç koyun popülasyonunda belirlenen TNP’lerin genetik yapısı PCR-RFLP ve DNA dizi analizi metodu ile incelenmiştir. Araştırmada toplam 450 örnek olmak üzere Sakız, Akkaraman ve İvesi ırkı koyunlardan 150’şer adet kan ve süt örneği kullanılmıştır. İvesi koyun ırkı β-LGB geni ekzon 2 bölgesi için monomorfik bulunmuştur. Sakız koyun ırkında AB genotipi ile süt verimi arasında önemli bir ilişki bulunurken (P<0.001), AA genotipli bireylerin yüksek yağ yüzdesine (P<0.001) sahip olduğu gözlenmiştir. Ayrıca bu çalışmada, β-LGB geni ekzon 2 bölgesine ait g.121C>A SNP ilk kez tanımlanmıştır. Bu araştırmada, Sakız koyun ırkında β-LGB geninin süt verimi üzerine önemli bir etkisi olduğu belirlenmiştir, ancak elde edilen bu bulguların bu konuda yapılacak ilişkilendirme çalışmaları ile doğrulanması gerekmektedir

Keywords

• β-laktoglobulin (β-LGB), koyun, süt verim özellikleri

Investigating the genetic polymorphism in the exon 2 region of ovine beta-lactoglobulin gene and its association with some milk traits

Abstract

β-lactoglobulin (β-LGB) gene is the major whey protein of ruminant and is synthesized by secreting cells of mammary gland during pregnancy and lactation and its biological function is still unclear. Therefore, the purpose of the study was to identify genotype frequencies of single nucleotide polymorphisms (SNPs) in ovine β-LGB gene exon 2 region and its possible association with milk traits in dairy sheep breeds. The genetic structures of the SNPs were examined by PCR-RFLP and DNA sequencing in three sheep populations. A hundred fifty blood and milk samples each from Sakiz, Akkaraman and Awassi ewes, with total of 450 samples were used in the experiment. Awassi sheep breed was found as monomorphic for β-LGB gene in exon 2 region. Genotype AB showed a strong association with milk yields content (P<0.001), whereas the animals carrying AA genotype had a higher fat percentage (P<0.001) value in the Sakiz sheep breeds. Furthermore, we have reported for the first time g.121C>A SNPs of the β-LGB gene for exon 2. In this research, β-LGB showed a significant effect on milk yield in the Sakiz ewes, but further studies are necessary to confirm the association reported here

Keywords

• β-lactoglobulin (β-LGB), milk production traits, sheep

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