Evaluation of antithrombotic and anti-ischemic effects of morinda citrifolia in renal autotransplantation in rats

Abstract

Although it is not performed as common as in humans, kidney transplantation is an effective treatment option for end-stage renal failure in dogs and cats. Ischemia/reperfusion injury is an inevitable result of kidney transplantation and it may cause delayed graft function that leads to a reduction in the longevity of allograft. In this study we aimed to evaluate the effects of Morinda citrifolia derived fruit juice on post-autotransplant ischemia/reperfusion injury. Twenty four adult, male Spraque-Dawley rats weighing 300-350 g were used. The rats were randomly divided into three groups of eight each. Rats in the Control Group were orally administered with saline solution (0.5 ml/day), for 7 days. Rats in M1 Group were orally administered with morinda citrifolia fruit juice (Alnoni, Hanojou-Europe Ltd, Dinxperlo, Holland), 0.5 ml/day, for 7 days whereas rats in M2 Group were orally administered with the same morinda citrifolia fruit juice (1 ml/day), for 7 days. In the 8th day, rats were anesthetized and orthotopic unilateral renal autotransplantation was performed. Rats were euthanized after 24 hour reperfusion and ischemic kidneys were sent for histopathological examination. Formalin-fixed paraffin-embedded tissue sections were evaluated and scored according to the parameters of tubular brush border loss, mononuclear cell infiltration, degeneration, hyalin formation in tubul lumens, thrombosis and tubular necrosis. Mononuclear cell infiltration in M2 group was found to be significantly different (P<0.05) than those of Control and M1 groups. Thrombosis was not observed in any of the histopathological sections. As a result, a significant improving effect on the ischemia/reperfusion injury of Morinda citrifolia fruit juice was not determined in rats after renal autotransplantation

Keywords

• Autotransplantation
• ischemia
• kidney
• morinda citrifolia
• reperfusion

Renal ototransplantasyon yapılan sıçanlarda morinda citrifolia ‘nın antitrombotik ve anti-iskemiketkilerinin değerlendirilmesi

Abstract

Böbrek transplantasyonu, son dönem böbrek yetmezliğinin tedavisi amacıyla, insanlardaki kadar yaygın olmamakla birlikte, kedi ve köpekler için de kullanılan bir renal replasman tedavisidir. İskemi/reperfüzyon hasarı ise böbrek nakillerinde kaçınılmaz olarak karşımıza çıkan ve gecikmiş gref fonksiyon bozukluğuna neden olarak nakledilen organın yaşam süresini kısaltan bir etken olarak varlığını sürdürmektedir. Bu çalışma ile tropikal bir meyveden elde edilen Morinda citrifolia meyve suyunun böbrek ototransplantasyonu sonrası oluşan iskemi/reperfüzyon hasarı üzerine etkilerini değerlendirmek amaçlanmıştır. Bu maksatla ağırlıkları 300-350 g arasında değişen, 24 adet Spraque-Dawley ırkı erkek sıçan, Kontrol, M1 ve M2 olmak üzere 3 gruba ayrıldı. Preoperatif olarak, Kontrol grubundaki sıçanlara % 0,9 NaCl solüsyonu (0,5 ml/gün), M1 grubuna Morinda citrifolia meyve suyu (0,5 ml/gün), M2 grubuna ise yine Morinda citrifolia meyve suyu (1 ml/gün) oral yolla 7 gün boyunca uygulandı. Sıçanlar 8. gün anesteziye alınarak, ortotopik unilateral böbrek ototransplantasyonu gerçekleştirildi. Sıçanlara, 24 saatlik reperfüzyon süresi sonunda ötanazi işlemi uygulandı ve iskemik böbrekler alınarak histopatolojik incelemeye gönderildi. Kesitler, histopatolojik muayene sonuçlarına göre, tubül fırçamsı kenar kaybı, mononükleer hücre infiltrasyonu, dejenerasyon, tubul lumenleri içinde hyalin oluşumu, tromboz ve tubüler nekroz parametreleri açısından değerlendirilerek skorlandı. Mononükleer hücre infiltrasyonu açısından M2 grubu ile M1 ve Kontrol grubu arasındaki fark ise önemli bulundu (P<0,05). Histopatolojik kesitlerin hiçbirinde tromboz varlığına rastlanmadı. Sonuç olarak sıçanlarda, Morinda citrifolia meyve suyunun böbrek ototransplantasyonları sonrası oluşan, iskemi/reperfüzyon hasarı üzerine önemli bir iyileştirici etkisi olmadığı sonucuna varıldı

Keywords

• Böbrek
• iskemi
• morinda citrifolia
• ototransplantasyon
• reperfüzyon

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