Determination of some acute phase proteins level in cattle with brucellosis

Abstract

The aim of the present study was to determine concentrations of some acute phase proteins (APP) and to investigate their diagnostic importance in cattle with brucellosis. For this purpose 20 cattle with brucellosis as working group and 10 healthy cattle as control group were used. Blood samples were collected from Jugular vein in tubes without anticoagulant. Blood samples were then centrifuged and the acquired serum samples were stored at -20 oC until future use. Samples were used for determine of concentration of haptoglobin (Hp), ceruloplasmin and albumin. Result of the present study indicated that the concentrations of positive APP including Hp, ceruloplasmin were significantly increased, and albumin concentration was significantly decreased in cattle with brusellosis when compared with healthy ones. In conclusion; in this study in animals infected with brucella occurs acute phase response and as a result Hp and ceruloplasmin synthesis increased while albumin synthesis is determined decreased

Keywords

• Acute phase proteins
• brucellosis
• cattle

Brusellozisli sığırlarda bazı akut faz proteinlerin düzeyinin saptanması

Abstract

Bu çalışmanın amacı brusella ile enfekte sığırlarda, bazı akut faz protein düzeylerinin belirlenmesi ve diagnostik önemlerinin araştırılmasıdır. Bu amaçla çalışmada 20 adet brusella ile enfekte ve 10 adet sağlıklı hayvan kullanıldı. Hayvanların V. jugularis’inden antikoagulansız tüplere kan örnekleri toplandı. Kan örnekleri -20 oC de saklandı. Hazırlanan serum örnekleri haptoglobin (Hp), albümin ve seruloplazmin ölçümlerinde kullanıldı. Yapılan analiz sonucunda brusella ile enfekte sığırlar ile kontrol grubundaki hayvanlar karşılaştırıldığında Hp ve seruloplazmin değerlerinin kontrol grubuna göre konsantrasyonunun yükseldiği, albümin değerinin ise kontrol grubuna göre konsantrasyonunun düştüğü belirlendi. Sonuç olarak; yapılan bu çalışmada brusella ile enfekte hayvanlarda akut faz yanıtın oluştuğu ve bunun sonucu olarak Hp ve seruloplazmin sentezinin arttığı, albümin sentezinin ise düştüğü belirlenmiştir

Keywords

• Akut faz proteinler
• brusellozis
• sığır

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