The investigation of calpastatin and thyroglobulin gene polymorphisms in some native cattle breeds

Abstract

In this study, calpastatin gene polymorphisms, known to have effect on meat tenderness, and thyroglobulin gene polymorphisms, known to affect marbling score, were investigated in some local cattle breeds raised in Turkey (Anatolian Native Black-ANT (n=36), Eastern Anatolian Red-EAR (n=51) and Turkish Grey-TG (n=52). The genetic variants in the bovine CAST and TG gene were analysed by using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. It was determined that while the calpastatin gene possessed the C allele, known to have a positive effect on meat tenderness, at a high frequency (0.67), suggesting that the local cattle breeds presented with a high potential for high quality meat production, the thyroglobulin gene was ascertained to possess the favourable T allele at a relatively low frequency (0.23), which could be increased with marker-assisted selection. It has been ascertained that the local cattle breeds investigated, and in particular the EAR had the potential to produce high quality red meat. Genetic analyses revealed the FIS, FIt and FSt values to be -0, 010, 0.045 and 0.055, respectively. These values showed that the local cattle breeds investigated displayed a high genetic diversity. Additionally, The allelic frequencies were calculated according to the Hardy-Weinberg equilibrium, and the differences between the cattle breeds were found to be statistically significant

Keywords

• Calpastatin
• Meat Quality
• Native cattle
• PCR-RFLP
• Thyroglobulin

Bazı yerli sığır ırklarında kalpastatin ve thyroglobulin gen polimorfizmlerinin araştırılması

Abstract

Türkiye’de bulunan bazı yerli sığır ırklarında (Yerli Kara-YK, n=36; Doğu Anadolu Kırmızısı-DAK (n=51) ve Boz IrkBI (n=52) et kalite özelliklerinden gevrekleşme üzerine etkili kalpastatin ve mermerleşme derecesi üzerine etkili thyroglobulin gen polimorfizmleri, polimeraz zincir reaksiyonu-restriksiyon parça uzunluk polimorfizmi (PCR-RFLP) metodu kullanılarak araştırılmıştır. Kalpastatin geninde et gevrekliği üzerine olumlu etkisi olan C allelinin yüksek frekansta (0.67) bulunduğu ve yerli sığır ırklarının yüksek potansiyel taşıdığı ,thyroglobulin için ise faydalı T allelinin nisbi olarak düşük frekansta bulunduğu (0.23) marker destekli seleksiyonla frekansının artırılabileceği ortaya çıkmıştır. Yerli sığır ırklarının özellikle DAK’ın kaliteli kırmızı et üretimi için potansiyel taşıdığı tespit edilmiştir. Genetik analizler sonucunda FIS, FIt ve FSt değerleri sırasıyla -0, 010, 0,045 ve 0,055 olarak tespit edilmiştir. Bu sonuçlara göre incelenen genler bakımından yerli sığır ırklarının yüksek genetik çeşitliliğe sahip olduğu belirlenmiştir. Ayrıca allel frekansları Hardy-Weinberg denge kuralına uygun olarak hesaplanarak ırklar arasında farkın önemli olduğu bulunmuştur

Keywords

• Kalpastatin
• Et kalitesi
• PCR-RFLP
• Thyroglobulin
• Yerli sığır ırkları

References

1. Anon (2010): NCBI Sequence Viewer 2.8.2. :http: // www. ncbi.nlm. nih. gov /projects/sviewer (14.09.2010).
2. Ailhaud G, Grimaldi P, Negrel, R (1992): Cellular and molecular aspects of adipose tissue development. Cellular and molecular aspects of adipose tissue development. Annual Review of Nutrition, 12, 207-233.
3. Barendse W (2000): Protocol for genotyping TG5 alleles using an MboI restriction endonuclease digest for the GTAC/T Molecular Laboratories, Research Road, St. Lucia.4072. Centre, Animal Genetics Gehrmann Mourao Figueiredo caracterization of Europen-zebu composite bovine using RFLP markers. Genet. Mol. Res. 4, 496-505. Genetic
4. Nei M (1972). Genetic distance between populations. Am. Nat. 106: 283-292.
5. Pareek CS, Zieba M, Michno J, Czaınik U, Zwierzchowski polymorphism within the candidate genes for dairy and beef traits in a panel of selected cattle breeds. J. Agrobiol. 25, 121-124. of SNP C>T
6. Rincon G, Medrano JF (2006): Assays for genotyping single nucleotide polymorphismus in the bovine CAPN1 gene. Animal Genetics. 37, 294-295.
7. Schenkel FS, Miller JR, Jiang Z, Mandell IB, Ye X (2006): Association of a single nucleotide polymorphism in the calpastatin gene with carcass and meat quality traits of beef cattle. J. Anim. Sci. 84, 291–299.
8. Zhang HM, Brown DR, Denise SK, Ax RL (1993): Rapid communication: polymerase chain reaction- restriction fragment length polymorphism analysis of the bovine somatotropin gene. J. Anim. Sci. 71, 2276-2279.