Effects of boron (sodium pentaborate), added instead of Tris components, on freezing and post-thaw quality of Angora buck semen

Abstract

In this research, we aimed to evaluate freezing and in vitro results of post-thawed Angora goat semen parameters using boron (sodium pentaborate, Na2O.5BO3.10H2O) instead of other components and as an additive in Tris extender. Mature Angora bucks, with proven fertility, accustomed to artificial vagina, and with normospermic values have been used in the study. Research was carried out with 9 different extenders: Boron extenders prepared by adding different amounts of Boron instead of glucose into standard Tris extender (GIB, 1-a and 1-b, 1-c, 1-d), the standard Tris extenders prepared by only addition of different amounts of Boron (BAD; 2-a, 2-b, 2-c, 2-d) and standard Tris extender (Control). Post-thaw spermatological examinations have been done with sperm analyzer (CASA) and results were recorded as follows; the highest total and progresive motility scores between 9 different extenders belonged to 1-c with 63,34 ± 2,32 and 1-d with %38,27± 0,74, highest VCL, VSL, VAP, LIN, STR and WOB average scores were found in 2-d extender (p<0,001).The percentage of lowest dead spermatozoa rate % 42,175± 1,59 was observed with GIB between main groups (p<0,01).Between extenders, lowest dead spermatozoa rates were recorded as % 36,04± 2,58 and % 36,17± 3,66 in 2-b and 1-c (p<0,001).When abnormal spermatozoa rates were examined, lowest abnormal spermatozoon rate belonged to 2-c extender with 21,29± 0,97 (p<0,001) and abnormal acrosome rates were obtained from %6,57± 1,29 1-a extender with (p<0,01). In conclusion, some positive effects of sodium pentaborate on post-thaw spermatological parameters of Angora buck semen have been demonstrated

Keywords

• Angora bucks
• boron
• semen
• spermatological parameters

Ankara tekesi spermasının dondurulmasında Tris sulandırıcı bileşenleri yerine katilan borun (sodyumpentaborat) çözüm sonu sperma kalitesi üzerine etkisi

Abstract

Bu araştırmada, Ankara tekesi spermasının dondurulması ve çözdürülmesinde, borun (sodyum pentaborat, Na2O.5B2O3.10H2O) Tris sulandırıcısına eklenerek ve bileşenleri yerine katılarak hazırlanmış solüsyonlarla, elde edilecek in vitro sonuçların değerlendirilmesi amaçlandı. Çalışma materyali olarak, dölverimi kanıtlanmış ve suni vaginaya sperma vermeye alıştırılmış ergin Ankara tekelerinin normospermik ejakülatları alındı. Çalışmada standart Tris sulandırıcısından glikoz çıkarılıp farklı miktarlarda bor eklenerek hazırlanmış sulandırıcılar (GYB; 1-a, 1-b, 1-c, 1-d), standart Tris sulandırıcısına farklı miktarlarda bor eklenerek hazırlanmış sulandırıcılar (BEK;2-a, 2-b, 2-c, 2-d) ve standart Tris sulandırıcısı (Kontrol) olmak üzere 9 farklı sulandırıcı kullanıldı. Sperma analiz sistemi (CASA) ile total motilite ve progresif motilite değerlerine bakıldığında sırasıyla en yüksek değerinlerin 63,34 ± 2,32 ile 1-c ve %38,27± 0,74 ile 1-d sulandırıcılarına VCL, VSL, VAP, LIN, STR, WOB verilerinin en yüksek 2-d sulandırıcısına ait olduğu belirlendi (p<0,001). Ölü spermatozoon oranı değerlendirildiğinde en düşük ölü spermatozoon oranının % 36,04± 2,58 ve % 36,17± 3,66 ile sırasıyla 2-b ve 1-c sulandırıcılarına ait olduğu gözlemlendi (p<0,001). Anormal spermatozoon oranları incelendiğinde GYB %27,60± 0,79, BEK % 27,89± 1,36 olarak bulundu (p<0,05). Akrozoma bağlı anormal spermatozoon değerleri ise en düşük oran %21,29± 0,97 ile 2-c sulandırıcısından elde edildi (p<0,001). Akrozoma bağlı anormal spermatozoon verilerinde ise en düşük oranın ise %6,57± 1,29 ile 1-a sulandırıcısına ait olduğu belirlendi (p<0,01). Sonuç olarak sodyum pentaborat’ın Ankara tekesi çözüm sonu spermatolojik parametrelere olan olumlu bazı etkileri ortaya konulmuştur

Keywords

• Ankara tekesi
• bor
• sperma
• spermatolojik özellikler

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