Evaluation of oxidative stress, hematological and biochemical parameters during Toxoplasma gondii infection in gerbils

Abstract

The aim of the present study was to investigate the alterations of oxidative stress, hematological and biochemical parameters in experimental infection caused by Toxoplasma gondii in gerbil. A total of 16 gerbil, 8 of which were control and 8 was infection group, were used in the study. The gerbils were infected by intraperitoneal inoculation of 5000 T. gondii RH strain tachyzoites. In group of, the gerbil were sacrificed at 7th day after inoculation. At the end of this period, blood samples collected and erythrocyte malondialdehyde (MDA) concentrations, superoxide dismutase (SOD), catalase (CAT) activities, plasma aspartat aminotransferase (AST) and alanine aminotranspherase (ALT) activities, total protein, albumin, globulin were determined. Besides, hematological parameters were analysed in whole blood. Aspartat aminotransferase and ALT activities and MDA concentrations and neutrophil percentage and total leukocyte counts increased significantly in infected group when compared to control. In infected group, SOD activities, albumin concentrations and lymhocyte percentage decreased when compared to control. The results of this study suggest that oxidative stress, hematological and biochemical alterations may contribute to the pathogenesis of toxoplasmosis in gerbils

Keywords

• Blood parameters
• gerbil
• oxidative stress
• Toxoplasma gondii

Gerbillerde Toxoplasma gondii enfeksiyonu süresince oksidatif stres, hematolojik ve biyokimyasalparametrelerin değerlendirilmesi

Abstract

Bu çalışma Toxsoplasma gondii (T.gondii) ile deneysel olarak enfekte edilmiş gerbillerde oksidatif stres ile hematolojik ve biyokimyasal parametrelerde meydana gelen değişikliklerin araştırılması amacıyla yapıldı. Çalışmada kontrol grubunda 8, enfeksiyon grubunda 8 olmak üzere toplam 16 tane gerbil kullanıldı. Gerbiller 5000 T. gondii RH takizoiti ile intraperitoneal yolla enfekte edildi. İnokülasyon sonrası 7. günde gerbiller sakrifiye edildi. Daha sonra kan örnekleri toplandı ve eritrosit malondialdehit (MDA) konsantrasyonu, süperoksit dismutaz (SOD) ve katalaz (CAT) aktiviteleri, plazma aspartat aminotransferaz (AST) ve alanin aminotransferaz (ALT) aktiviteleri, total protein, albumin ve globin düzeyleri belirlendi. Bunun yanısıra, tüm kanda hematolojik parametlerin analizi yapıldı. Kontrol ile karşılaştırıldığında enfekte grupta, AST ve ALT aktiviteleri ve MDA konsantrasyonu, nötrofil oranı ve toplam akyuvar sayısında anlamlı bir artış bulundu. Enfekte grupta, SOD aktivitesi, albumin konsantrasyonu ve lenfosit oranında kontrol ile karşılaştırıldığında düşme belirlendi. Bu çalışmanın sonuçlarına göre, gerbillerde toksoplazmozisin patogenezinde oksidatif stresin, hematolojik ve biyokimyasal değişikliklerin katkısının olduğu söylenebilir

Keywords

• Gerbil
• kan parametreleri
• oksidatif stres
• Toxoplasma gondii

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