Ovariektomi ve ovaryohisterektomi yapılan köpeklerde serum seruloplazmin ve paraoksonaz-1düzeyleri

Abstract

Bu çalışmanın amaçları köpeklerde ovariektomi (OVE) ve ovaryohisterektomi (OVH) ile ilişkili akut faz yanıtının şiddetinin değerlendirilmesi ve paraoksanaz-1 (PON-1) aktivitesinin araştırılmasıydı. Çalışmada toplam 21 adet sağlıklı dişi köpek kullanıldı. Köpekler rastgele OVH (n=11) ve OVE (n=10) yapılmak üzere iki gruba ayrıldı. Operasyon öncesinde ve operasyon sonrası 4. günde alınan periferal kan örneklerinde serum seruloplazmin düzeyi ve paraoksonaz-1 aktivitesi ölçüldü. Sonuçlar gruplar içerisinde ve gruplar arasında karşılaştırıldı. Paraoksanaz-1 ve seruloplazmin arasında muhtemel korelasyon değerlendirildi. Operasyon öncesi serum seruloplazmin seviyeleri OVE ve OVH gruplarında sırasıyla 0.80±0.17 and 1.65±0.35 U/ml olarak ölçüldü (P>0.05). Operasyon sonrası 4. günde serum seruloplazmin seviyesi OVH grubunda (1.95±0.47 U/ml) OVE grubuna kıyasla daha yüksek bulundu (0.69±0.10 U/ml; P<0.05). Operasyon öncesi ve sonrası serum seruloplazmin seviyesi çalışma grupları içerisinde farklılık göstermedi (P>0.05). Operasyon öncesi ve sonrasında serum PON-1 aktivitesi OVE ve OVH gruplarında sırasıyla 1001.56±44.60 U/ml ve 1119.10±87.99 U/ml olup (P>0.05), operasyon sonrası serum PON-1 aktivitesi OVH grubunda (1065.15±46.00 U/ml) OVE grubuna kıyasla daha yüksekti (941.22±32.57 U/ml; P<0.05). PON-1 aktivitesi ile serum seruloplazmin konsantrasyonu arasında bir korelasyon yoktu (P=.250; r=0.06). Sonuç olarak, OVE grubunda postoperatif akut faz yanıtının şiddeti OVH grubuna göre daha düşüktü. Buna ek olarak, köpeklerde OVE ve OVH operasyonu sonrası 4. günde PON-1 aktivitesinde değişiklik ve PON-1 aktivitesi ile serum seruloplazmin konsantrasyonları arasında bir ilişki bulunmadı

Keywords

• Dişi köpek
• Seruloplazmin
• Ovariektomi
• Ovaryohisterektomi
• Paraoksonaz-I.

Serum ceruloplasmin and paraoxonase-1 levels in ovariectomized and ovariohysterectomized dogs

Abstract

The objectives of this study were to evaluate the severity of acute phase reaction and to investigate the paraoxanase-1 (PON-1) activity associated with ovariectomy (OVE) and ovariohysterectomy (OVH) in dogs. A total of 21 adult healthy dogs were included. The dogs were randomly divided into two groups and either ovariectomized (n=10) or ovariohysterectomized (n=11). Peripheral blood samples were collected preoperatively and on postoperative fourth day for serum ceruloplasmin (cp) and paraoxanase-1 activity measurements. Results were compared within and between groups. Potential correlation between paraoxanase-1 and ceruloplasmin was assessed. Preoperative mean serum ceruloplasmin levels in OVE and OVH groups were 0.80±0.17 and 1.65±0.35 U/ml, respectively (P>0.05). On postoperative 4th day, serum ceruloplasmin levels in OVH group (1.95±0.47 U/ml) was higher compared to OVE group (0.69±0.10 U/ml; P<0.05). Pre- and postoperative serum ceruloplasmin levels were not different within study groups (P>0.05). Preoperative serum paraoxanase-1 activity in OVE and OVH groups were 1001.56±44.60 U/ml and 1119.10±87.99 U/ml, respectively (P>0.05). Higher postoperative serum paraoxanase-1 activity was observed in OVH group (1065.15±46.00 U/ml) compared to OVE group (941.22±32.57 U/ml; P<0.05). Pre and postoperative serum paraoxanase-1 activity was not different within study groups (P>0.05). Paraoxanase-1 activity was not correlated with concentration of ceruloplasmin (P=.250; r=0.06). In conclusion, OVE is superior to OVH in terms of reducing the severity of postoperative acute phase response. In addition, OVE and OVH does not alter canine paraoxanase-1 activity on postoperative fourth day and paraoxanase-1 activity is not associated with serum concentration of ceruloplasmin in dogs

Keywords

• Bitch
• Ceruloplasmin
• Ovariectomy
• Ovariohysterectomy
• Paraoxonase-I

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