Effect of Mentofin application on the clearance of Mycoplasma gallisepticum (MG) from naturally infected layer chickens’ trachea

Abstract

Aim of this study was to determine if Mentofin would have any effect on Mycoplasma gallisepticum (MG) clearance from the tracheal epithelium of chickens in commercial layer flocks, which were naturally infected with MG. Results indicated that, compared to the control group, there was a significant and continuous decline in MG infection in chickens of Mentofingroup determined by culture and Real-Time Polymerase Chain Reaction (MGrPCR) (P<0,05). Serology results in the control group indicated an increase in MG positivity from 25% to 40% (P>0,05), while there was no change in the Mentofin group (P>0,05). Culture results for MG positivity decreased from 85% to 5% in the Mentofin group, while this decrease was from 80% to 35% in the control group (P<0,05). There was a prominent decrease from 100% to 20% in MGrPCR positives in the Mentofin group (P<0,05) compared to a non-significant change observed from 95% to 80% in the control group (P>0,05). Results of this study indicate that Mentofin clearly had an effect on MG clearance from the tracheal epithelium, supported by detection of decline in MG infection in layers

Keywords

• Chicken
• Mentofin
• Mycoplasma gallisepticum

Mentofin uygulamasının Mycoplasma gallisepticum (MG) ile doğal infekte yumurtacı tavuklarıntrakeasından arınması üzerine etkisi

Abstract

Bu çalışmanın amacı Mentofin’in Mycoplasma gallisepticum (MG) ile doğal infekte ticari yumurtacı sürülerin trakeal epitellerinden MG’un arınması üzerine etkisinin belirlenmesidir. Sonuçlar, kontrol grubu ile karşılaştırıldığında Mentofin grubundaki tavuklarda MG infeksiyonunda kültür ve Real-Time Polymerase Chain Reaction (MGrPCR) ile belirlenen belirgin ve sürekli bir düşüş olduğunu göstermiştir (P<0,05). Seroloji sonuçları kontrol grubunda MG pozitiflik %25’den %40’a yükselirken (P>0,05), Mentofin grubunda bir değişiklik olmamıştır (P>0,05). Kültür sonuçlarındaki MG pozitiflik Mentofin grubunda %85’den %5’e düşerken, kontrol grubunda bu düşüş %80’den %35’e olmuştur (P<0,05). Mentofin grubundaki MGrPCR pozitifliğinde belirgin şekilde olan %100’den %20’ye düşüş (P<0,05), kontrol grubunda %95’den %80’e (P>0,05) olan hafif bir düşme olarak gözlenmiştir. Çalışma sonuçları Mentofin’in trakeal epitelden MG arınmasında belirgin bir etkisinin olduğunu yumurtacılarda MG infeksiyonunda düşmenin belirlenmesi ile desteklenen şekilde göstermiştir

Keywords

• Mentofin
• Mycoplasma gallisepticum
• tavuk

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