Testicular morphology and in vitro evaluation of frozen epididymal sperm of Anatolian buffalo[*]

Abstract

Epididymal sperm may be available as a source of spermatozoa for artificial insemination and in vitro embryo production in many species. The aim of the study was to investigate epididymal sperm freezing on spermatological parameters of Anatolian buffaloes and in vitro evaluation of frozen epididymal sperm for assisted reproductive biotechnologies. The testes and epididymides were collected from 10 Anatolian buffalo bulls (about 3-4 years of age) in Samsun Province. The testes and epididymides from each bull were removed immediately after slaughtering and transported to the laboratory on ice (+4 °C) within 40 min for further processing. After morphological measurements of testes and epididymides were determined, the spermatozoa were collected from cauda epididymides. The percentage of sperm progressive motility and abnormal spermatozoa of fresh and post-thaw sperm samples were evaluated. There was no significant difference between fresh and frozen-thawed sperm samples for progressive sperm motility and head-acrosome anomalies of spermatozoa. The middle part (P<0.001), tail (P<0.001) and total (P<0.001) abnormal spermatozoa rates of frozen-thawed sperm samples were higher than those of fresh sperm samples. In conclusion, collected spermatozoa from cauda epididymides of Anatolian buffalo bull may be used as fresh or frozen for artificial insemination, in vitro embryo production or intra cytoplasmic sperm injection

Keywords

• Anatolian buffalo
• epididymal sperm
• epididymis
• morphometry
• testis

Anadolu mandalarında testiküler morfoloji ve dondurulmuş epididimal spermanın in vitrodeğerlendirilmesi

Abstract

Epididimal sperma birçok türde suni tohumlama ve in vitro embriyo üretiminde spermatozoa kaynağı olarak kullanılabilir. Yapılan bu çalışmanın amacı Anadolu Mandaları’ndan alınan epididimal spermanın dondurulmasının bazı spermatolojik parametreler üzerine etkilerinin incelenmesi ve yardımcı üreme teknolojileri için dondurulan epididimal spermanın kullanılabilirliğinin in vitro değerlendirilmesidir. Samsun İlinde kesilen (yaklaşık 3-4 yaşlı) toplam 10 adet erkek Anadolu Mandasından kesim sonrasında testisler ve epididimisler hemen alınarak buz içinde (+4 °C) 40 dakika içerisinde daha sonraki işlemler için laboratuara getirildi. Testis ve epididimislerin morfolojik ölçüleri belirlendikten sonra spermatozoa cauda epididimisten toplandı. Nativ ve çözüm sonu örneklerde progresif spermatozoa motilitesi ve anormal spermatozoa yüzde oranları değerlendirildi. Nativ ve dondurulmuş çözülmüş sperma örnekleri arasında progresif spermatozoa motilitesi ve spermatozoa baş-akrozom anomalileri yönünden herhangi bir fark tespit edilmedi. Dondurulmuş çözülmüş spermadaki orta kısım (P<0,001), kuyruk (P<0,001) ve total (P<0,001) anormal spermatozoa oranları, nativ spermaya göre daha yüksek bulunmuştur. Sonuç olarak, Anadolu Mandasının cauda epididimisinden elde edilen spermatozoa nativ veya dondurulmuş epididimal sperma olarak suni tohumlamada, in vitro embriyo üretiminde ya da intra sitoplazmik spermatozoa enjeksiyonu gibi yardımcı üreme biyoteknolojilerinde kullanılabilir

Keywords

• Anadolu mandası
• epididimal sperma
• epididimis
• morfometri
• testis

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