Koyun ve farelerde kist hidatik proteinlerinin karşılaştırmalı analizi ve antijenik proteinlerde glikoprotein varlığının değerlendirilmesi

Abstract

Bu çalışmada koyun ve farelerde kist hidatik proteinlerinin, antijenik proteinler ve antijenik glikoproteinler yönünden karşılaştırılması amaçlandı. Bu amaçla 20’şer adet enfekte ve 10’ar adet sağlıklı koyun ve fare kullanıldı. Hidatidozisli koyunlardan ve deneysel enfekte farelerden elde edilen kist sıvısı, kist membranı ve protoskolekslere ait proteinler Sodyum dodesil sülfat poliakrilamid jel elektroforez (SDS-PAGE) yöntemi ile ayırt edildi. Ayırt edilen proteinlerden hangilerinin hastalığa spesifik antijenler içerdiği immunblotting tekniği ile belirlendi. Koyun kist sıvısı, membran ve protoskolekslerde 116 kDa ile 26 kDa arasında, fare kist sıvısı ve membranlarında 106 ile 30 kDa arasında değişik sayılarda antijenik bantlar tespit edildi. Koyun kist sıvısı ile fare kist sıvısı arasındaki ortak antijenik bantların 40 kDa ve 26 kDa, koyun kist mebranı ile fare kist mebranı arasındaki ortak antijenik bantların 48 kDa ve 40 kDa olduğu belirlendi. Belirlenen antijenik proteinlerden, glikoprotein yapısında olanlar ve bunların hangi şekerleri bulundurdukları glikan kitleri ile belirlendi. Koyunlarda her üç kist materyalinde de (kist sıvısı, kist membranı, protoskoleks) 116 kDa, 68kDa, 48 kDa’luk proteinlerin, farelerde her iki kist materyalinde de (kist sıvısı, kist membranı) 106 kDa, 48, 40 kDa’luk proteinlerin ortak glikoproteinler olduğu tespit edildi. Bu glikoproteinlerin mannoz, galaktoz, N-asetil galaktozamin, sialik asit gibi şekerlerden bir ya da bir kaçını içerdikleri belirlendi. Koyun ve farelerin kist materyalleri arasında yapılan karşılaştırmada ortak bantların, molekül büyüklüğü, glikoprotein varlığı ve şeker yapıları yönünden benzerlikler ve farklılıklar gösterdiği görüldü

Keywords

• Antijenik protein
• fare
• glikoprotein
• kist hidatik
• koyun

Comparative analysis of sheep and mice hydatid cyst proteins and evaluation of the glycoproteinoccurence in the antigenic proteins

Abstract

The aim of this study is to compare the hydatid cyst proteins of mice and sheep in terms of antigenic proteins and antigenic glycoproteins. For this reason 20 each infected sheep and mice and 10 each healthy sheep and mice were used. Cyst fluid, cyst membrane and proteins from protoscolex which were derived from hydatidosis infected sheeps and experimentaly infected mice were separated by SDS-PAGE. Antigenic proteins were determined by immunblotting technique. Moleculer weights of antigenic proteins from cyst fluid, cyst membrane and protoscolex of sheep were determined in size between 116 kDa and 26 kDa by SDSPAGE. Cyst fluid and cyst membrane of mice, molecular weights of antigenic proteins were found between 106 kDa and 30kDa. Common antigenic bands of hydatid fluid from sheep and mice were found to be 40 kDa and 26 kDa. Common antigenic bands of hydatid membrane from sheep and mice found to be 48 kDa and 40 kDa. Sugars of antigenic proteins in glycoprotein structure were determined by glycan kits. Glycan analysis of samples showed that 116 kDa, 68 kDa and 48 kDa proteins in each three cyst material (cyst fluid, cyst membrane and protoscolex) obtained from sheep were found to be common glycoproteins. In addition to this, 106 kDa, 48 kDa and 40 kDa proteins in each two cyst material (cyst fluid, cyst membrane) obtained from mice were also found to be common glycoproteins. It was determined that these glycoproteins include one or more sugar structures such as mannose, galactose, N-acetyl galactosamine and sialic acid. In the context of sheep and mice cyst material comparison, it was determined that common proteins shows similarities and dissimilarities in molecular weight, glycoprotein and sugar structures in general

Keywords

• Antigenic protein
• cyst hydatid
• glycoprotein
• mice
• sheep

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