Antimicrobial susceptibility and serotype distribution of Listeriamonocytogenes isolates obtained from raw milk cheese samples sold inNigde

Abstract

This study was designed to evaluate public health risks in respect to listeriosis due to consumption of local raw milk cheese sold in villages and public bazaars of Nigde. In addition, this study aims to contribute to the treatment process of listeriosis by determining serotype distribution and antimicrobial susceptibility profiles of the isolates. In the study, two of cheese (1 %) samples of total 200 raw milk cheese samples (100 white cheeses and 100 tulum cheeses) were found contamined with L. monocytogenes. Serotype distribution of the isolates were determined as 1/2a (white cheese isolate) and 4a (tulum cheese isolate). Finally, antimicrobial resistance profiles of the isolates against 11 antibiotics (tetracycline, ciprofloxacin, gentamicin, ampicillin, erythromycin, trimethoprim, vancomycin, chloramphenicol, nalidixic acid, penicillin G and sulphanilamide) were determined and the MIC values were specified by a microdilution method. Both of the isolates were found to be resistant to nalidixic acid but sensitive to the others analysed. As a result, although low L. monocytogenes prevalance rates were detected in this study, it is a public health concern to isolate serotype 1/2a which is secondly incriminated serotype to cause human listeriosis. In addition, it is important in terms of public health to continue surveillance of emerging antimicrobial resistance among L. monocytogenes isolated especially from foods of animal origin though incidence of antibiotic resistance in L. monocytogenes isolates is rare

Keywords

• Antimicrobial susceptibility
• cheese
• Listeria monocytogenes
• microdilution
• serotype distribution

Niğde’de satışa sunulan çiğ sütten yapılmış peynir örneklerinden elde edilen Listeria monocytogenesizolatlarının serotip dağılımının ve antimikrobiyel duyarlılık profilinin belirlenmesi

Abstract

Bu çalışma, Niğde ili köy ve pazarlarında tüketime sunulan çiğ sütten yapılmış peynirlerin listeriozis açısından taşıdığı halk sağlığı risklerini değerlendirmek ve peynirlerden elde edilen izolatların serotip dağılımını ve antimikrobiyel duyarlılık profillerini belirleyerek tedavi sürecine katkıda bulunmak amacıyla planlanmıştır. Çalışma sonucunda 200 çiğ sütten yapılmış peynir örneğinden (100 beyaz peynir ve 100 tulum peyniri) ikisinin (% 1) L. monocytogenes ile kontamine olduğu bulunmuştur. L. monocytogenes izolatlarının serotip dağılımı 1/2a (beyaz peynir izolatı) ve 4a (tulum peyniri izolatı) olarak belirlenmiştir. Son olarak izolatların 11 antibiyotiğe (tetrasiklin, siprofloksasin, gentamisin, ampisilin, eritromisin, trimetoprim, vankomisin, kloramfenikol, nalidiksik asit, penisilin G ve sülfonilamid) karşı direnç profilleri belirlenmiş ve MIC değerleri mikrodilüsyon metodu ile ortaya konmuştur. İzolatların her ikisinin de nalidiksik asite dirençli, diğer antibiyotiklere duyarlı olduğu tespit edilmiştir. Sonuç olarak incelenen örneklerde L. monocytogenes prevalansının çok düşük düzeylerde bulunmasına karşın insan listeriozisinden ikinci sırada sorumlu tutulan 1/2a serotipinin belirlenmiş olması halk sağlığı açısından risk unsuru olarak değerlendirilmiştir. Ayrıca, L. monocytogenes izolatları arasındaki antimikrobiyel dirençlilik nadir olsa da özellikle hayvansal gıdalardan elde edilen L. monocytogenes izolatlarının antimikrobiyel direnç profillerinin sürekli gözetim altında tutulması halk sağlığı açısından önem arzetmektedir

Keywords

• Antimikrobiyel duyarlılık
• Listeria monocytogenes
• mikrodilüsyon
• peynir
• serotip dağılımı

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