Investigation of Q Fever in Kilis and Shamil goats by ELISA and Touchdown-PCR

Abstract

This study was carried out on Kilis and Shamil goats to determine the different stages of Q fever-chronic and acute- in serum samples by indirect ELISA and in blood samples by touchdown-PCR, respectively. A total of 92 serum samples comprising 46 Kilis and 46 Shamil goats was examined and 7.60%, 4.34% and 10.8% seropositivity were determined for total serum samples, Kilis and Shamil goat serum samples, respectively. Of the examined 92 blood samples belonging to 46 Kilis and 46 Shamil goats, none of them yielded 687 bp PCR products by touchdown-PCR. The positive ELISA titers were thought to be the evidence of previous infection. The province of Kilis localized in South-East Anatolian Region of Turkey was decided to be a risky region for Q fever

Keywords

• Q fever
• goat
• ELISA
• touchdown-PCR

Kilis ve Halep keçilerinde ELISA ve Touchdown-PCR ile Q Humması’nın araştırılması

Abstract

Bu çalışma, Kilis ve Halep keçilerinde, Q Humması’nın farklı dönemlerini -kronik ve akut- sırasıyla; serum örneklerinde indirek ELISA, kan örneklerinde touchdown-PCR ile ortaya koymak için planlanmıştır. Kırkaltı Kilis ve 46 Halep keçisinden oluşan toplam 92 serum örneği incelendi ve toplam serum örnekleri, Kilis ve Halep keçi serumlarında seropoziflik sırasıyla, 7.60%, 4.34% ve 10.8% bulundu. Kırkaltı Kilis ve 46 Halep keçisine ait toplam 92 kan örneğinin, hiçbirinde touchdown-PCR ile 687 bp’lık PCR ürünü tespit edilmedi. Pozitif ELISA titrelerinin daha once geçirilen infeksiyona kanıt olduğu düşünüldü. Türkiye’de Güneydoğu Anadolu Bölgesi’nde lokalize olan Kilis ilinin Q fever yönünden riskli bir bölge olduğuna karar verildi

Keywords

• Q humması
• keçi
• ELISA
• touchdown-PCR

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