Kuru döneme çıkarken enfekte ve sağlıklı meme loblarında California Mastitis Test ve somatik hücre sayısı ile bakteriyolojik muayene sonuçlarının karşılaştırılması

Abstract

Bu çalışmada kuruya çıkarma sırasında California Mastitis Test (CMT) sonuçlarının somatik hücre sayımı (SHS) ve bakteriyolojik muayene sonuçları ile karşılaştırılması ve CMT’nin kuru dönem başlangıcında enfekte meme loblarının belirlenmesinde etkinliğinin ortaya konması amaçlandı. Bu amaçla, 28 adet Holştayn ırkı ineğe ait 112 meme lobundan alınan 109 adet süt örneği değerlendirildi, 3 meme kör olduğu için süt örneği alınamadı. Kuruya çıkarmadan önceki son sağımda klinik muayenede normal olan meme loblarından alınan süt örneklerine CMT yapıldı ve sonuçlar aynı kişi tarafından yorumlandı. Bu uygulamadan sonra her meme lobundan SHS ve bakteriyolojik muayene için süt örnekleri alındı. CMT sonucuna göre 83 meme lobu negatif (N), 8 meme lobu şüpheli (T), 15 meme lobu + ve 3 meme lobu ise ++ reaksiyon verdi. CMT ile SHS sonuçları karşılaştırıldığında, CMT negatif meme loblarında ortalama SHS 155.178 hücre/ml, şüphelilerde 221.750 hücre/ml, + ve ++ reaksiyon verenlerde sırasıyla 635.400 ve 1.782.000 hücre/ml idi. Herhangi bir bakteri üreyen ve üremeyen meme loblarında ortalama somatik hücre sayıları ise sırasıyla 195.350 hücre/ml ve 304.400 hücre/ml idi. Bakteri üremesi olan ve olmayan gruplara ait ortalama somatik hücre sayıları arasındaki fark istatistiksel açıdan anlamlı değildi (p>0.05). Sonuç olarak kuruya çıkarılan ineklerde subklinik meme içi enfeksiyonların belirlenmesinde CMT’nin tek başına yeterli olmadığı, CMT sonuçlarının bakteriyolojik muayene sonuçları ile birlikte değerlendirilmesi gerektiği kanısına varıldı

Keywords

• Bakteriyolojik muayene
• CMT
• kuru dönem
• somatik hücre sayısı
• sütçü inek

Comparision of results of California Mastitis Test, somatic cell counts and microbiologicalexamination in healthy and infected quarter in cows at dry-off.

Abstract

The aim of this study was to compare California Mastitis Test (CMT) findings with Somatic Cell Count (SCC) and bacteriological examination at the dry-off, and to investigate the effectiveness of the CMT to detect the infected quarters at the beginning of the dry period. For this purpose, 109 milk samples collected from 112 udder quarters of 28 Holstein cows were evaluated, 3 milk samples could not take from these quarters owing to blindness. CMT was made on milk samples taking from clinically normal quarters before the last milking and the results was interpreted by the same person. After this examination milk samples were collected from each quarter for SCC and bacteriological isolation. Of the samples, 83 were CMT (-), 8 were trace (T), 15 were CMT (+), 3 were CMT (++). According to comparision of CMT and SCC results, the mean SCC was 155.178 cell/ml, 221.750 cell/ml, 635.400 cell/ml, 1.782.000 cell/ml in CMT (-), trace (T), CMT (+), CMT (++) quarters, respectively. The mean SCC of any bacteria isolated and non-isolated udder quarters were 195.350 cell/ml and 304.400 cell/ml, respectively. Difference in SCC levels between infected and non-infected groups was not significant statistically (p>0.05). In conclusion, only CMT was not adequate to determine subclinical intramammary infections at the dry-off cows, and to evaluate the findings of CMT with the results of bacteriological examination

Keywords

• Bacteriological examination
• CMT
• dairy cow
• dry period
• somatic cell count

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