Akdeniz’de yakalanan Dülger balığındaki Anisakis pegreffii (Nematoda: Anisakidae) enfeksiyonununmoleküler identifikasyonu ve patolojisi

Abstract

Çalışmanın materyalini Akdeniz’de Antalya körfezi avlanma sahasında yakalanan bir dülger balığı Zeus faber (Linnaeus, 1758) oluşturdu. Zeus faber dikkatli bir şekilde diseke edildi ve karın boşluğu, karaciğer ve kaslar Anisakidae ailesindeki nematod larvaları yönünden incelendi. Makroskopik olarak karın boşluğunda sekiz larva ile karaciğer yüzeyinde kapsüle olmuş 7 larva bulundu. Makroskobik incelemede, solgun görünümde olan karaciğer yüzeyinde kanamalara ve kapsül altında 0,3 cm çapında kistlere rastlandı. Histopatolojik incelemede, hepatositlerde şiddetli dejenerasyon ve nekroz tanımlandı. Karaciğer kapsülü altında fibröz kapsülle çevrili Anisakis larvaları gözlendi. Morfolojik olarak tanımlanan Anisakis Tip I larvalar tür teşhisi için PZR-RFLP ile moleküler olarak incelendi. Tüm Anisakis larvalar PZR-RFLP ile Anisakis pegreffii olarak teşhis edildi. Bu çalışma ile Türkiye sularında avlanan dülger balığında Anisakis pegreffii’nin moleküler identifikasyonu ve paratenik ya da ara konakdaki patolojik bulguları ilk kez tanımlandı

Keywords

• Anisakis pegreffii
• patoloji
• PZR-RFLP
• Zeus faber

Pathology and molecular identification of Anisakis pegreffii (Nematoda: Anisakidae) infection in the John Dory, Zeus faber (Linnaeus, 1758) caught in Mediterranean Sea

Abstract

One specimen of John Dory, Zeus faber (Linnaeus, 1758), was caught from commercial sites at the costs of Gulf of Antalya at Mediterranean Sea, Turkey. Zeus faber was dissected carefully. After examination Anisakidae larvae were recovered from in the abdominal cavity, liver and muscles. A total of eight larvae were found in the abdominal cavity and seven larvae encapsulated on surface of liver at necropsy. In the macroscopic examination, liver was seen to pale and surface of the capsula was haemorhagic with subcapsular cysts including larvae in 0.3 cm diameter. In the histopathologic examination severe degeneration and hepatic necrosis was identified. Just below the liver capsula, Anisakis larvae were seen covered with fibrous capsula. The larvae morphologically identified as Anisakis larvae Type I were subjected to further molecular characterization by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to identify the species. All Anisakis larvae were identified at species level by PCR-RFLP as belonging to A. pegreffii. Within the present study, for the first time, we report on the molecular identification of A. pegreffii larvae from John Dory, Z. faber, and we describe their pathological significance in a paratenic or intermediate hosts in Turkish waters

Keywords

• Anisakis pegreffii
• pathology
• PCR-RFLP analysis
• Zeus faber

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