The effect of preoperative antibiotic or antibiotic + vitamin C administrations on the inflammatory and oxidative state in the rabbits with experimentally induced pyometra

Abstract

In this study, rabbits were used as a model for canine pyometra to test the effects of preoperative therapy in the reduction of postoperative stress. Pyometra were induced in 18 healthy rabbits by the operative administration of Pasteurella multocida inoculum. Beginning on the third day after inoculation Group I (n=6) received enrofloxacin (2,5 mg/kg, s.c.), Group II (n=6) recieved enrofloxacin (2,5 mg/kg, s.c.) + vitamin C (100 mg, s.c.) injections for three days. Ovariohysterectomy were performed in groups I and II following this medical treatment. Group III (n=6) as the control group, did not recieve any medical therapy and were operated on the third day after inoculation. Blood samples were collected before inoculation, on the day of operation, 24th h, 72th h and at 7th day following the operations. Ceruloplasmin (CERU), malondialdehyde (MDA), vitamin C, haptoglobin (Hp) and serum amyloid-A (SAA) levels were analyzed. In the postoperative 72th h period mean CERU level of Group III were found significantly higher (p<0,05) than Group I and II. Mean MDA level were significantly higher in Group III on the day of operation compared to the treatment groups (p<0,05). SAA levels rised in all of the groups following inoculation, on the day of operation (p<0,05) which is followed by a gradual decline until the 7th day. Overall CERU and MDA levels tend to be higher in the control group compared to the treatment groups whereas Hp and SAA levels were not significantly different. These findings may indicate that preoperative therapy may reduce the oxidative stress mildly however it did not significantly alter the acute phase protein response definitively and a significant positive effect of both preoperative treatments compared to the control group could not be pronounced

Keywords

• Acute phase proteins
• antioxidants
• ovariohysterectomy
• pyometra
• rabbit

Tavşanlarda deneysel oluşturulan pyometra olgularında, preoperatif antibiyotik veya antibiyotik + Cvitamini enjeksiyonlarının yangısal ve oksidatif duruma olan etkileri

Abstract

Bu çalışmada pyometra operasyonu sonrası stresin azaltılmasında preoperatif sağaltımın etkisini araştırmak amacıyla tavşanlar model olarak kullanıldı. Bu amaçla onsekiz sağlıklı tavşanda Pasteurella multocida’ nın operatif yöntemle uterusa inokulasyonu gerçekleştirildi. İnokulasyondan sonra 3. günden başlanarak Grup I’ e (n=6) enrofloksasin (2,5 mg/kg, s.c.), Grup II’ ye (n=6) aynı dozda enrofloksasin ve vitamin C (100 mg, s.c.) enjeksiyonları 3 gün süreyle devam ettirilmiş ve bundan sonra ovaryohisterektomi uygulanmıştır. Kontrol grubu olan Grup III’ e (n=6) medikal sağaltım uygulanmamış ve inokulasyondan sonra üçüncü günde ovaryohisterektomi uygulanmıştır. Kan örnekleri inokulasyondan önce, ovaryohisterektomi gününde, postoperatif 24. saat, 72. saat ve 7. günde alınmıştır. Bu örneklerde serüloplazmin (CERU), malondialdehit (MDA), vitamin C, haptoglobin (Hp) ve Serum Amiloid A (SAA) düzeyleri belirlenmiştir. Serüloplazmin bütün gruplarda inokulasyonu takiben yükselirken Grup I ve II’ de postoperatif 72s’ de düşmüş ancak Grup III’ de yüksek bulunmuştur (p<0,05). Yine Grup II’ de MDA düzeyleri operasyon gününde sağaltım gruplarına göre yüksektir (p<0,05). SAA düzeyleri zamana bağlı olarak bütün gruplarda operasyon gününde yükselmiş daha sonra postoperatif 7. güne kadar düşmüştür. Genel olarak CERU ve MDA düzeylerinin kontrol grubunda diğer gruplara göre daha yüksek olduğu, Hp ve SAA düzeylerinde ise gruplar arasında önemli bir farklılık görülmediği saptanmıştır. Bu bulgular preoperatif sağaltımın oksidatif stresi önlemede sınırlı düzeyde etkili olurken akut faz yanıtı üzerinde önemli düzeyde etkili olmadığını göstermektedir

Keywords

• Antioksidanlar
• akut faz proteinleri
• ovaryohisterektomi
• pyometra
• tavşan

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