Histomorphology of the porcine oviduct

Abstract

The present study was aimed at the light and electron microscopic examination of the epithelium of the porcine oviduct, determination of the histochemical structure of the mucus secreted by epithelial cells, and demonstration of the localisation and distribution of endothelial nitric oxide synthase (eNOS) activity during the different stages of the oestrous cycle. The oviducts of 5 healthy adult sows constituted the material of the study. Histochemically, it was observed that, increased glycosaminoglycan (GAG) secretion during the oestrus phase, was in particular stored in the crypts of the isthmus. Electron microscopic examination revealed that, the increased amount of secretory material, which accumulated in secretory cells during the oestrus phase, was stored in two different types of secretory granules. Immunohistochemically, it was ascertained that eNOS was not expressed in either the ampulla or the fimbria during the oestrus and luteal phases, but was expressed in the isthmus only during the oestrus phase

Keywords

• eNOS
• histochemistry
• oviduct
• pig

Domuzlarda oviduktun histomorfolojisi

Abstract

Bu çalışma; domuzlarda ovidukt epitelinin yapısını, ışık ve elektron mikroskobik olarak incelemek, epitel hücreleri tarafından salgılanan mukus içeriğinin histokimyasal yapısını ortaya koymak ve östrus siklusunun farklı dönemlerindeki endotelyal nitrik oksit sentaz (eNOS) aktivitesinin, lokalizasyonu ve dağılımını belirlemek amacıyla yapıldı. Çalışmada 5 adet sağlıklı, erişkin dişi domuzdan alınan ovidukt, materyal olarak kullanıldı. Histokimyasal olarak; östral dönemde artan glikozaminoglikan (GAG) salgısının, özellikle istmusdaki kriptlerde depolandığı görüldü. Elektron mikroskobik olarak; östral dönemde sekretorik hücrelerin sitoplazmalarında artan salgı materyalinin, iki tip salgı granülünde toplandığı belirlendi. İmmunohistokimyasal olarak; hem östral hem de luteal dönemde ampulla ve fimbriyada eNOS ekspresyonu görülmez iken, istmusda ise yalnızca östral dönemde eNOS ekspresyonu saptanmıştır

Keywords

• Domuz
• eNOS
• histokimya
• ovidukt

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