Investigation of some toxins genes and methicillin resistance gene in Staphylococcus aureus isolatesfrom cows with subclinical mastitis

Abstract

The aim of this study was to investigate the genes of some staphylococcal enterotoxins and Panton-Valentine leukocidin and methicillin resistance of Staphylococcus aureus strains isolated from subclinical bovine mastitis. In this study, 60 S. aureus isolates were used and the genes were detected by PCR. seg-sei, sej, sec and seh genes were detected in eight (13.33%) isolates, six (10.00%) isolates, four (6.67%) isolates and three (5.00%) isolates, respectively. Sixteen (26.67%) isolates were found to harbor one or more toxin genes. None of the investigated isolates harbored sea, seb, sed, see, and tsst genes, while four (6.6%) isolates and two (3.30%) isolates had Panton-Valentine leukocidin gene and mecA gene, respectively. As a conclusion, it was determined that S. aureus strains isolated from cows with subclinical mastitis could produce enterotoxin and harbor mecA gene. This situtiation could be a potential risk factor for public health

Keywords

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Subklinik mastitisli inek sütlerinden izole edilen Staphylococcus aureus izolatlarında bazı toksin genleri ve metisilin direnç geninin araştırılması

Abstract

Bu çalışma subklinik mastitisli inek sütlerinden izole edilen Staphylococcus aureus izolatlarında bazı stafilokokal enterotoksin, Panton-Valentine lökosidin genleriyle metisilin direnç geninin araştırılması amacıyla yapılmıştır. Araştırmada 60 S. aureus izolatı kullanılmış ve genlerin belirlenmesinde polimeraz zincir reaksiyonundan yararlanılmıştır. İzolatların sekizinde (% 13.33) seg-sei, altısında (% 10.00) sej, dördünde (% 6.67) sec ve üçünde (% 5.00) ise seh geni belirlenmiştir. Toplam on altı (% 26.67) izolatta bir veya daha fazla enterotoksin geni tespit edilmiştir. İncelenen izolatlarda enterotoksin genlerinden sea, seb, sed, see ve tsst belirlenemezken dördünde (% 6.6) Panton-Valentine lökosidin geni ve ikisinde (% 3.30) metisilin direnç geni belirlenmiştir. Sonuç olarak subklinik mastitisli sütlerde bulunan S. aureus izolatlarının enterotoksin üretebilecekleri ve metisilin direnç geni taşıyabilecekleri belirlenmiştir. Bu durum halk sağlığı açısından önemli bir risk oluşturmaktadır

Keywords

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