Presence of IS/1494/06 genotype-related infectious bronchitis virus in breeder and broiler flocks in Turkey

Abstract

In this study, infectious bronchitis (IB)-suspect problems were observed in 2 broiler and 8 breeder chicken flocks vaccinated with H120 strain, and a total of 220 tracheal swabs were taken from 60 broiler chickens of 2 flocks (30 swabs/flock), and 160 breeder chickens of 8 flocks (20 swabs/flock) to detect infectious bronchitis virus (IBV) by a reverse transcriptase polymerase chain reaction (RT-PCR) and to isolate IBV from chickens showing respiratory sings and nephritis lesions. Detection of 8 Turkish IBVs from the tracheal swab samples directly by RT-PCR was confirmed by both IBV isolation from allantoic fluids of embryonated eggs and by the same RT-PCRs from these samples. Basic Local Alignment Search Tool (BLAST) results revealed that all 8 sequences from our Turkish IBV isolates were related to that of EU780077 (IS/1494/06 IBV strain) with a 99% identity. Phylogenetic and alignment analyses of the sequences of two representative isolates as TR_IS1494_06_B12011 from one broiler flock and TR_1494_06_7201 from one breeder flock also confirmed this close relation to IS/1494/06 IBV genotype. As a result, Turkish IBVs have been isolated for the first time from chickens, which were not protected from IB despite IBV H120 strain vaccination, and genotyped as IS/1494/06 in Turkey suggesting the transfer of this IBV from the countries of Middle East

Keywords

• IS/1494/06 Infectious bronchitis virus
• chicken
• Turkey

Türkiye’de damızlık ve broyler sürülerde IS/1494/06 genotipi-ilişkili infeksiyöz bronşit virusununvarlığı

Abstract

Bu çalışmada H120 suşu ile aşılanmış 2 broyler ve 8 damızlık sürüde infeksiyöz bronşit şüpheli problemlere rastlanmış 2 sürüye ait 60 broyler tavuktan (30 svap/sürü) ve 8 sürüye ait 160 damızlık tavuktan (20 svap/sürü) özellikle solunum bulguları ile nefrit lezyonları gösterenlerden toplam 220 trakeal svap infeksiyöz bronşit virusu (IBV)’nu reverse transcriptase polymerase chain reaction (RT-PCR) ile belirlemek ve IBV’yi izole etmek amacı ile alındı. IBV’nin trakeal svap örneklerinden direkt olarak RT-PCR ile deteksiyonu embryolu yumurtaların allantoik sıvılarından IBV izolasyonu ve bu örneklerden RT-PCR yapılması ile doğrulandı. Basic Local Alignment Search Tool (BLAST) sonuçları 8 Türk izolatının EU780077 (IS/1494/06 IBV suşu) ile %99 benzerlik gösterdiğini ortaya koydu. Broyler sürüden TR_IS1494_06_B12011 ve damızlık sürüden TR_1494_06_7201 olarak seçilen temsili iki izolatın dizilerinin filogenetik ve sıralama analizleri de IS/1494/06 IBV genotipine yakınlığı doğruladı. Sonuç olarak, Türkiye’de IBV H120 suşu ile aşılamaya rağmen IB’den korunamayan tavuklardan ilk defa izole edilmesi ve IS/1494/06 olarak genotiplendirilmesi bu IBV’nin Orta Doğu ülkelerinden taşınma olasılığını akla getirmektedir

Keywords

• IS/1494/06 İnfeksiyöz bronşit virusu
• tavuk
• Türkiye

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