Analysis of cyst fluid antigens from different hosts for the serodiagnosis of hydatidosis

Abstract

The aims of this study were to determine the antigenic differences of hydatid cyst fluid from different animals and to investigate the effect of the origin of antigenic materials on the diagnosis of hydatidosis in humans and mice. For this purpose, the proteins of hydatid cyst fluid (HCF) isolated from the liver of sheep and donkeys and secondary hydatid cysts fluid (SHCF) from albino mice infected with protoscolices isolated from sheep and donkeys were separated by SDS-PAGE. This study was performed at the University of Ankara, Faculty of Veterinary Medicine, Department of Parasitology. For comparing antigenic profiles and detecting specific protein bands of those antigens, 10 infected and 10 non-infected human sera were tested for cystic echinococcosis (CE), and 16 positive and 9 negative mice sera were tested for secondary hydatidosis (SH) by using Western blotting technique. The specific protein band for mice sera was detected as 8 kDa (100%) for sheep’s and donkey’s hydatid cyst fluid. The specific proteins for human sera were detected at 24 kDa (100%) and at 8 kDa (100%) for sheep’s and donkey’s hydatid cyst fluid respectively. No band was detected in negative mice and human sera. It was concluded that SHCF antigen from mice could be considered useful as being easy to provide and give good results for the diagnosis of CE

Keywords

• Cyst fluid antigen
• diagnosis
• donkey
• mice
• sheep
• Western blotting

Hydatidosisin serodiagnosisinde çeşitli konaklardan elde edilen antijenlerin analizi

Abstract

Çalışmanın amacı farklı hayvanların hidatik kist sıvısının antijenik farklılıklarını saptamak ve insan ve farelerde hidatidosisin identifikasyonunda antijenik materyalin orijininin etkisini araştırmaktır. Bu amaçla Ankara Üniversitesi Veteriner Fakültesi Parazitoloji Anabilim Dalında yapılan çalışmada, koyun ve eşek karaciğerlerinden elde edilen hidatik kist sıvılarındaki proteinler ile yine koyun ve eşek orijinli protoskolekslerle enfekte albino farelerden elde edilen sekonder hidatik kist sıvılarındaki proteinler SDS-PAGE yöntemi ile ayrılma işlemine tabi tutulmuştur. Bu antijenik yapıların spesifik protein bantlarının tespiti ve karşılaştırılması için kistik ekinokokoz yönünden 10 enfekte, 10 enfekte olmayan insan serumu ve sekonder hidatidosis için 16 pozitif ve 9 negatif fare serumu Western blot yöntemi ile test edilmiştir. Fare serumlarında koyun ve eşek kist sıvıları için tek spesifik protein bandının 8 kDa (%100) olduğu saptanırken, insan serumları kullanıldığında koyun hidatik kist sıvısı için 24 kDa (%100), eşek hidatik kist sıvısı için 8 kDa (%100) bandın spesifik olduğu tespit edilmiştir. İnsan ve fare negatif serumlarında banda rastlanmamıştır. Sonuç olarak kistik ekinikokozun teşhisinde farelerden elde edilen sekonder hidatik kist sıvılarının iyi sonuç verdiği ve elde edilmesinin kolay olması yönüyle hastalığın tanısında rahatça kullanılacağı ortaya konmuştur

Keywords

• Kist sıvısı antijeni
• tanı
• eşek
• fare
• koyun
• Western blotting

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