Expression of platelet activating factors and its receptor in cat uterus during early pregnancy

Abstract

Platelet activating factors (PAF1β, PAF1γ, PAF:ah, PAF-R) have a significant role during mammalian pregnancy process. This study aimed to investigate expression profiles of PAF gene family in cat uterus during pregnancy. Tissue samples were collected via ovariohysterectomy at days 7 (Gr1; n=3), 18-20 (Gr2; n=7), 24-25 (Gr3; n=5) after mating from the different regions of the uterus. Relative expression levels of genes were quantified using real-time qRT-PCR. A mixed model was fitted on the normalized data and Least Significant Difference (LSD) test was employed to determine significant differences between groups. Expressions of PAF genes were assessed in the cat uterus during pregnancy around implantation period; however, no significant differences were detected between the preimplantation and the implantation stage. Similarly, no significant difference concerning mRNA expression was assessed between different parts of the uterus during pregnancy. In conclusion, PAF genes are expressed in the cat uterus during early pregnancy. However, this expression is not associated with changes in the uterus during early pregnancy

Keywords

• PAF
• cat
• pregnancy
• qRT-PCR

Kedi uterusunda, platelet aktive edici faktörler ve reseptörünün erken gebelik dönemindekiekspresyonu

Abstract

Platelet aktive edici faktörler (PAF1β, PAF1γ, PAF:ah, PAF-R), memeli hayvanların gebelik sürecinde önemli bir role sahiptir. Bu çalışmanın amacı, gebelik süresince kedi uterusunda PAF gen ailesi ekspresyon profillerini araştırmaktır. Doku örnekleri çiftleşme sonrası 7 (Gr1; n=3), 18-20 (Gr2; n=7), 24-25. (Gr3; n=5) günlerde yapılan ovariyohisterektomi operasyonları ile uterusun farklı bölgelerinden toplandı. Nispi mRNA ekspresyon seviyeleri real-time PZR kullanılarak tespit edildi. Normalize edilmiş veriler karışık model kullanılarak analiz edildi ve istatistikî olarak farklı olan gruplar Asgari Önemli Fark (AÖF) testi ile tespit edildi. Kedi uterusunda implantasyon sürecinde PAF genlerinin eksprese olduğu gözlenmiştir ancak; istatistikî olarak çalışma grupları ve uterus bölgeleri arasında herhangi bir fark bulunmamıştır. Sonuç olarak, kedilerde erken gebelik sürecinde uterusta PAF genleri eksprese olmaktadır. Ancak; bu ekspresyonların erken gebelik döneminde uterusta görülen değişikliklerle ilişkisi yoktur

Keywords

• PAF
• kedi
• gebelik
• qRT-PZR

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