Monitorize effects of chemicals that used in the study on MDCK cells with phase contrast microscope

Abstract

The aim of this study is to monitorize effects of chemicals that used in the study on MDCK cells with phase contrast microscope. MDCK cells that were treated with CaCl2, MgSO4, Sodiumcitrate, Na2HPO4 and NaOx/ CaCl2 solutions. MDCK cells that not treated with any chemicals are used as control group. Calcium islets were observed in cells that treated with CaCl2 and calcium oxalate crystals which binded to the cells were observed in cells that treated with NaOx/CaCl2 solution. The MDCK cells that treated with Na-citrate solution, there was any difference was found when compared with control cells. The MDCK cells, treated with Na2HPO4 solution, necrotic areas were found

Keywords

• MDCK cell line
• CaCl2
• Na-sitrat
• MgSO4
• NaHPO4
• Calcium oxalate

Farklı kültür ortamlarının MDCK hücrelerine etkilerinin faz kontrast mikroskop ile gösterilmesi

Abstract

Deneysel olarak Kalsiyumklorür (CaCl2), Magnezyumsülfat (MgSO4), Sodyumsitrat, Sodyumhidrojenfosfat (Na2HPO4) ve Kalsiyum okzalat solusyonlarına maruz bırakılan böbrek hücrelerindeki etkiler faz kontrast mikroskopla görüntülenmiştir. Hiçbir kimyasalla muamele edilmeyen Madin Darby Canine Kidney (MDCK) hücreleri kontrol grubu olarak kullanılmıştır. CaCl2 eklenmis grubun faz kontrast mikroskobik görüntüsünde yer yer kalsiyum birikintilerinin, kalsiyum okzalat eklenen grupta ise hücrelere tutunmus halde kalsiyum okzalat kristallerinin olustuğu gözlenmistir. MgSOçözeltisi eklenen MDCK hücrelerinde yer yer dejenere bölgelere rastlanmıştır. Na-sitrat çözeltisi eklenen MDCK hücreleri, kontrol hücreleriyle karşılaştırıldığı zaman büyük bir farklılığa rastlanmamıştır. Na2HPO4 çözeltisi eklenen MDCK hücrelerinde ise hücreler arasında yer yer nekrotik alanlara rastlanmıştır

Keywords

• MDCK hücre hattı
• CaCl2
• Na-sitrat
• MgSO4
• Na2HPO4
• Kalsiyum okzalat

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