Sığır, koyun ve insanlardan izole edilen Brucella suşlarının konvansiyonel ve moleküler biyotiplendirmesi

Abstract

Bu çalışmada Kayseri bölgesinde sığır ve koyunlardaki atık olgularında Brucella’nın rolü araştırıldı ve ağırlıklı alt-tür ve biyovarlar konvansiyonel ve moleküler biyotiplendirme metotları ile belirlendi. Çalışmada 61 adet sığır, 64 adet koyun atık materyali ve ayrıca 50 insan kanından izole edilmiş Brucella spp. incelendi. On yedi (%27.9)’si sığır ve 12’si (%18.7) koyun atık örneklerinden olmak üzere toplam 29 adet Brucella spp. izole edildi. Hayvan ve insan izolatları konvansiyonel yöntemler ve Enhanced AMOS-ERY PCR olmak üzere iki farklı yöntem ile tiplendirildi. Çalışma kapsamında sığırlardan izole edilen tüm Brucella spp.’nin B.abortus biyovar 3’e ait olduğu konvansiyonel yöntemlerle saptandı ve bu izolatların tamamının moleküler olarak biyovar 3b olduğu tespit edildi. Koyun ve insan orijinli izolatların ise konvansiyonel ve moleküler olarak B.melitensis biyovar 3 oldukları tespit edildi. Bu çalışma ile Türkiye’nin Kayseri İli’nde, insan, sığır ve koyunlarda Brucellosis’e neden olan predominant Brucella biovarları belirlenmiş oldu. Bu bulguların Brucellosise yönelik koruma ve kontrol aktiviteleri için yararlı olabileceği düşünülmektedir

Keywords

• Biyotiplendirme, Brucella, enhanced AMOS-ERY PCR

Conventional and molecular biotyping of Brucella strains isolated from cattle, sheep and human

Abstract

In this study, the role of Brucella spp. in cattle and sheep abortions among Kayseri region was investigated and predominant subspecies and biovars in this region were determined by conventional and molecular biotyping methods. For this purpose, 61 cattle and 64 sheep abortion material and also 50 human blood isolates were examined. A total of 29 Brucella spp. 17 (27.9%) and 12 (18.7%) of which were isolated from cattle and sheep specimens, respectively) were isolated from animal sources. Both animal and human isolates were typed by conventional and Enhanced AMOS-ERY PCR methods. All Brucella spp. strains isolated from cattle were found to belong to B. abortus biovar 3 and biovar 3b using conventional and molecular typing methods, respectively. All sheep originated Brucella spp. strains and human originated Brucella spp. strains were found to belong to B.melitensis biovar 3 using both conventional and molecular methods. As a result, predominant biovars causing brucellosis in human, cattle and sheep in Kayseri, Turkey were detected. These findings were considered to be useful in prevention and controlling activities for Brucellosis in Turkey

Keywords

• Biotyping, Brucella, enhanced AMOS-ERY PCR

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