Chemical composition and antibacterial activity of essential oils from different parts of endemic Bupleurum L. species

Abstract

The essential oils of Bupleurum heldreichii Boiss. & Bal., Bupleurum sulphureum Boiss. & Bal., Bupleurum turcicum Snogerup, and Bupleurum lycaonicum Snogerup flowers, fruits and roots were obtained using hydrodistillation and microdistillation techniques and their chemical compositions were analyzed by GC and GC/MS systems, simultaneously. The antibacterial activity of the oils which obtained by hydrodistillation was assessed with micro-dilution assays. The main components of B. heldreichii were germacrene D (% 47.5-48.4) in flowers and fruits, and hexadecanoic acid (% 46.2) in roots. The main components of B. sulphureum found undecane (% 14.0-20.2) in flowers and fruits, and calarene (% 26.9) in roots. The main components of B. turcicum were heptanal (% 33.2-23.5) in flowers and fruits, and pentacosane (% 9.0) in roots. The main components of B. lycaonicum were tridecane (% 14.9-37.3) in flowers and roots, spathulenol (% 14.4) in fruits. The essential oils of B. heldreichii, B. sulphureum, B. turcicum obtained from flowers and fruits, B. lycaonicum from fruits used in the study did not have any effect against bacteria. The MIC values of essential oils of the roots for the bacterial strains tested, which were sensitive to the essential oils of roots of B. heldreichii, B. sulphureum and B. turcicum were in the ratio of 2 mg/ml. This investigation showed that the antibacterial activity of B. heldreichii, B. sulphureum and B. turcicum was attributed to the essential oil of roots, thus they can be a potential medicinal resource

Keywords

• Antibacterial activity
• endemic Bupleurum species
• essential oil composition
• microdilution

Endemik Bupleurum L. türlerinin farklı kısımlarının uçucu yağlarının kimyasal kompozisyonu veantibakteriyel aktivitesi

Abstract

Bupleurum heldreichii Boiss. & Bal., Bupleurum sulphureum Boiss. & Bal., Bupleurum turcicum Snogerup ve Bupleurum lycaonicum Snogerup’un çiçek, meyve ve köklerinin uçucu yağları hidrodistilasyon ve mikrodistilasyon yöntemleriyle elde edildi ve kimyasal bileşimleri GC ve GC/MS sistemleri ile eşzamanlı olarak incelendi. Hidrodistilasyon ile elde edilen uçucu yağların antibakteriyel aktiviteleri mikrodilüsyon yöntemiyle belirlendi. B. heldreichii’nin çiçek ve meyvelerinde germakren D (% 47.5-48.4), köklerinde hekzadekanoik asit (% 46.2) ana bileşenler olarak belirlendi. Ana bileşenler B. sulphureum’un çiçeklerinde ve meyvelerinde undekan (% 14.0-20.2), köklerinde kalaren (% 26.9) olarak bulundu. B. turcicum’un çiçeklerinde ve meyvelerinde heptanal (% 33.2-23.5), köklerinde pentakosan (% 9.0) ana bileşenler olarak belirlendi. B. lycaonicum’un çiçeklerinde ve köklerinde tridekan (% 14.9-37.3), meyvelerinde spatulenol (% 14.4) ana bileşenler olarak bulundu. B. heldreichii, B. sulphureum, B. turcicum’un çiçek ve meyvelerinden, B. lycaonicum’un meyvelerinden elde edilen uçucu yağların çalışmada kullanılan bakterilere karşı etkisinin olmadığı görüldü. B. heldreichii, B. sulphureum ve B. turcicum’un kök uçucu yağlarının test edilen bakteri suşları için MİK değerleri 2 mg/ml’dir. Bu araştırma B. heldreichii, B. sulphureum ve B. turcicum’un kök uçucu yağlarının antibakteriyel aktivitesinin olduğunu ve potansiyel tıbbi kaynak olabileceğini gösterdi

Keywords

• Antibakteriyel aktivite
• endemik Bupleurum türleri
• mikrodilüsyon
• uçucu yağ bileşimi

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